Electrical conductivity and ion diffusion in porcine meniscus: effects of strain, anisotropy, and tissue region

Kleinhans, Kelsey L.; McMahan, Jeffrey B.; Jackson, Alicia R.

doi:10.1016/j.jbiomech.2016.06.011

Cited by 7 publications

(5 citation statements)

References 30 publications

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“…Tissue composition was investigated to identify potential relationships with measured mechanical properties. The average φ water and φ GAG were within the ranges typically observed for porcine and human meniscus, 11,15,[53][54][55][56] see Table 2. Similar to other studies, medial menisci had significantly higher φ water and φ GAG than lateral.…”

Section: Resultssupporting

confidence: 69%

Heterogeneity of dynamic shear properties of the meniscus: A comparison between tissue core and surface layers

Schwartz

Morejon

Gracia

et al. 2022

Journal Orthopaedic Research

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Damage to the meniscus has been associated with excessive shear loads. Aimed at elucidating meniscus pathophysiology, previous studies have investigated the shear properties of the meniscus fibrocartilaginous core. However, the meniscus is structurally inhomogeneous, with an external cartilaginous envelope (tibial and femoral surface layers) wrapping the tissue core. To date, little is known about the shear behavior of the surface layers. The objective of this study was to measure the dynamic shear properties of the surface layers and derive empirical relations with their composition. Specimens were harvested from tibial and femoral surface layers and core of porcine menisci (medial and lateral, n = 10 each). Frequency sweep tests yielded complex shear modulus (G*) and phase shifts (δ). Mechanical behavior of regions was described by a generalized Maxwell model. Correlations between shear moduli with water and glycosaminoglycans content of the tissue regions were investigated. The femoral surface had the lowest shear modulus, when compared to core and tibial regions. A 3-relaxation times Maxwell model satisfactorily interpreted the shear behavior of all tissue regions. Inhomogeneous tissue composition was also observed, with water content in the surface layers being higher when compared with tissue core. Water content negatively correlated with shear properties in all regions. The lower measured shear properties in the femoral layer may explain the higher prevalence of meniscal tears on the superior surface of the tissue. The heterogenous behavior of the tissue in shear provides insight into meniscus pathology and has important implications for efforts to tissue engineer replacement tissues.

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Section: Resultssupporting

confidence: 69%

Heterogeneity of dynamic shear properties of the meniscus: A comparison between tissue core and surface layers

Schwartz

Morejon

Gracia

et al. 2022

Journal Orthopaedic Research

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“…Averaged meniscal Φ w , pooled from all groups, was 0.770 ± 0.032, which is comparable to previous measurements on human [ 9 , [33] , [34] , [35] , [36] , [37] , [38] ] and on porcine tissues [ 9 , [39] , [40] , [41] ]. Medial meniscal samples had significantly higher Φ w on average than lateral samples (p < 0.001).…”

Section: Discussionsupporting

confidence: 86%

Effect of molecular weight and tissue layer on solute partitioning in the knee meniscus

Morejon¹,

Schwartz²,

Best³

et al. 2023

Osteoarthritis and Cartilage Open

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Section: Methodsmentioning

confidence: 99%

“…The samples were first subjected to an unconfined compression test with a universal testing machine at compression strengths of 10% and 20% strain, selected based on previous studies. 23,24 The 10% strain was selected as a physiological compression strength and the 20% strain as a mechanical overload. 25 After achieving the preload condition of 0.1% strain, the indenter head speed was set at a 0.5 mm/s until 20% compression was reached.…”

Section: Biomechanical Analysesmentioning

confidence: 99%

Comparison of High‐Hydrostatic‐Pressure Decellularized Versus Freeze‐Thawed Porcine Menisci

Watanabe

Mizuno

Matsuda

et al. 2019

Journal Orthopaedic Research

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The meniscus functions as a load distributor and secondary stabilizer in the knee, and the loss of the meniscus increases the risk of osteoarthritis. Freeze‐thawed menisci are used in clinical practice to replace defective menisci; however, the disadvantages of freeze‐thawed tissues include disease transmission and immune rejection. In this study, we decellularized menisci using high hydrostatic pressure (HHP) and compared the decellularized menisci with freeze‐thawed menisci. Porcine menisci were either pressurized at 1,000 MPa for 10 min and then washed with DNase solution or frozen at −80°C for 2 days and thawed. These menisci then underwent in vitro histological, biochemical, and biomechanical comparisons with native menisci. The HHP‐treated and freeze‐thawed menisci were also subcutaneously implanted in a pig, and later harvested for histological analysis. The numbers of histologically detected cells were significantly lower and the amount of biochemically detected DNA was approximately 100‐fold lower in HHP‐treated than in native and freeze‐thawed menisci. The compression strength of the HHP‐decellularized menisci was decreased after 1 and 50 cycles at 20% strain but was unchanged in the freeze‐thawed menisci. After implantation, the numbers of multinucleated giant cells were significantly lower around the HHP‐treated menisci than around the freeze‐thawed menisci. Recellularization of the HHP‐decellularized menisci was confirmed. Thus, although the HHP‐decellularized menisci were mechanically inferior to the freeze‐thawed meniscus in vitro, they were immunologically superior. Our study is the first to demonstrate the use of HHP for decellularization of the meniscus. © 2019 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society. J Orthop Res 37:2466–2475, 2019

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Electrical conductivity and ion diffusion in porcine meniscus: effects of strain, anisotropy, and tissue region

Cited by 7 publications

References 30 publications

Heterogeneity of dynamic shear properties of the meniscus: A comparison between tissue core and surface layers

Heterogeneity of dynamic shear properties of the meniscus: A comparison between tissue core and surface layers

Effect of molecular weight and tissue layer on solute partitioning in the knee meniscus

Comparison of High‐Hydrostatic‐Pressure Decellularized Versus Freeze‐Thawed Porcine Menisci

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