Electrical and mechanical activity recorded from rabbit urinary bladder in response to nerve stimulation.

Creed, Kate E.; Ishikawa, Shiro; Ito, Yushi

doi:10.1113/jphysiol.1983.sp014666

Cited by 106 publications

(85 citation statements)

References 20 publications

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“…This is in general agreement with previous studies of guinea-pig bladder (De Sy, 1971;Moss & Burnstock, 1985), rabbit and pig bladder (Sibley, 1984) in which atropine resistance has been observed at stimulation frequencies < 10 Hz. Creed et al (1983) have also noted that the late atropine-sensitive component of the electrical response of rabbit bladder to nerve stimulation increased in amplitude and duration with higher frequencies of stimulation. Kasakov & Burnstock (1983) showed that a,fi-methylene ATP abolished the contractile response at stimulation frequencies > 2-4Hz.…”

Section: Discussionmentioning

confidence: 95%

“…ATP has also been shown to be stored in guinea-pig bladder, although it is not known whether it is co-located with acetylcholine in cholinergic nerves, or is in separate purinergic nerves (Burnstock, 1983). Creed et al (1983) have recorded the electrical responses of rabbit bladder smooth muscle to single stimuli of the intrinsic nerves, and have observed an initial electrical response consisting of an excitatory junction potential (ej.p.) giving rise to a spike, and a delayed more prolonged depolarization of 250-300ms latency and 2s duration.…”

Section: Introductionmentioning

confidence: 99%

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Electrical and mechanical responses of guinea‐pig bladder muscle to nerve stimulation

Brading

Mostwin

1989

British J Pharmacology

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1 The electrical and mechanical responses to transmural stimulation of intrinsic nerves have been recorded from smooth muscle strips dissected from the dome of the guinea-pig bladder, by use of intracellular microelectrodes, and conventional tension recording techniques. 2 Stimulation of intrinsic nerves evoked action potentials in all cells studied. Hyperpolarization of the cells by extracellular current injection revealed subthreshold excitatory junction potentials (ej.ps) in about a quarter of the cells studied. 3 Action potentials could still be evoked in the presence of atropine and neostigmine, but were abolished after desensitization of the cells to a,#-methylene ATP, a stable analogue of ATP.4 In the presence of neostigmine, the evoked action potential was followed by a slow depolarization of the membrane. The mechanical response increased in amplitude and duration. 5 The contractile response to transmural nerve stimulation was reduced but not abolished in the presence of either atropine or desensitizing doses of a,fi-methylene ATP. Atropine was more effective at high frequencies of stimulation () 30Hz), and a,#-methylene ATP at low frequencies ( < 15 Hz). In combination the drugs abolished the response. 6 The results suggest that the mechanical response to excitatory nerve stimulation is biphasic. The early transient reponse is elicited by ej.ps and evoked spikes, is resistant to atropine, but sensitive to desensitization of purinoceptors. The late response is mediated through muscarinic receptors, involves little membrane depolarization, and is unaffected by desensitization of purinoceptors. These responses are analogous to the responses seen in rabbit bladder, and in the sympathetically innervated rat tail artery and guinea-pig vas deferens.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Electrical and mechanical responses of guinea‐pig bladder muscle to nerve stimulation

Brading

Mostwin

1989

British J Pharmacology

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“…Early experiments recording electrical activity with the sucrose-gap technique (Creed et al, 1983;Fujii, 1988) pre-supposes that electrical current flows between cells. Furthermore direct measurement of the cable properties of detrusor demonstrated a space constant of more than 1 mm (Seki et al, 1992;.…”

Section: Figure 4 Near Herementioning

confidence: 99%

The physiological function of lower urinary tract smooth muscle

Fry

Meng

Young

2010

Autonomic Neuroscience

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“…K ϩ efflux through large-conductance Ca 2ϩ -activated K ϩ (BK) channels and voltage-gated K ϩ (K V ) channels is responsible for the repolarization phase of the action potential (29,63). K V channels contribute to the afterhyperpolarization phase along with small-conductance Ca 2ϩ -activated K ϩ (SK) channels (12,21,25). Because BK and SK channels play such a central role in mediating action potential repolarization, blocking them with toxins such as iberiotoxin (IbTX) or apamin, respectively, potentiates the detrusor smooth muscle action potential and greatly increases the force of the resulting contractions (32,44,69).…”

mentioning

confidence: 99%

Unique properties of muscularis mucosae smooth muscle in guinea pig urinary bladder

Heppner¹,

Layne²,

Pearson³

et al. 2011

American Journal of Physiology-Regulatory, Integrative and Comp

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The muscularis mucosae, a type of smooth muscle located between the urothelium and the urinary bladder detrusor, has been described, although its properties and role in bladder function have not been characterized. Here, using mucosal tissue strips isolated from guinea pig urinary bladders, we identified spontaneous phasic contractions (SPCs) that appear to originate in the muscularis mucosae. This smooth muscle layer exhibited Ca 2ϩ waves and flashes, but localized Ca 2ϩ events (Ca 2ϩ sparks, purinergic receptor-mediated transients) were not detected. Ca 2ϩ flashes, often in bursts, occurred with a frequency (ϳ5.7/min) similar to that of SPCs (ϳ4/min), suggesting that SPCs are triggered by bursts of Ca 2ϩ flashes. The force generated by a single mucosal SPC represented the maximal force of the strip, whereas a single detrusor SPC was ϳ3% of maximal force of the detrusor strip. Electrical field stimulation (0.5-50 Hz) evoked force transients in isolated detrusor and mucosal strips. Inhibition of cholinergic receptors significantly decreased force in detrusor and mucosal strips (at higher frequencies). Concurrent inhibition of purinergic and cholinergic receptors nearly abolished evoked responses in detrusor and mucosae. Mucosal SPCs were unaffected by blocking smallconductance Ca 2ϩ -activated K ϩ (SK) channels with apamin and were unchanged by blocking large-conductance Ca 2ϩ -activated K ϩ (BK) channels with iberiotoxin (IbTX), indicating that SK and BK channels play a much smaller role in regulating muscularis mucosae SPCs than they do in regulating detrusor SPCs. Consistent with this, BK channel current density in myocytes from muscularis mucosae was ϳ20% of that in detrusor myocytes. These findings indicate that the muscularis mucosae in guinea pig represents a second smooth muscle compartment that is physiologically and pharmacologically distinct from the detrusor and may contribute to the overall contractile properties of the urinary bladder. calcium imaging; large-conductance calcium-activated potassium channel; potassium channels; calcium flashes; spontaneous phasic contractions THE URINARY BLADDER is a hollow, muscular organ that serves two functions: urine storage and elimination. The bladder wall is composed of detrusor smooth muscle, which accounts for a large fraction of the bladder mass, and a layer of transitional epithelial cells known as the urothelium, which lines the luminal surface of the urinary bladder. The detrusor smooth muscle has been extensively characterized and is clearly responsible for the majority of the contractile properties of the urinary bladder (1, 69). The urothelium, which was originally thought to simply act as a passive, impermeable barrier that prevents the movement of solutes from the urine into the bloodstream, is now recognized to possess a broad range of sensory and signal transduction functions that greatly influence bladder physiology (for a review, see Ref. 5).The detrusor exhibits two primary modes of contractile behavior: nerve-evoked contractions and spontaneo...

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Electrical and mechanical activity recorded from rabbit urinary bladder in response to nerve stimulation.

Cited by 106 publications

References 20 publications

Electrical and mechanical responses of guinea‐pig bladder muscle to nerve stimulation

Electrical and mechanical responses of guinea‐pig bladder muscle to nerve stimulation

The physiological function of lower urinary tract smooth muscle

Unique properties of muscularis mucosae smooth muscle in guinea pig urinary bladder

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