Electrical activity induced by nitric oxide in canine  colonic circular muscle

Keef, Kathleen D.; Anderson, UA; O'Driscoll, Kate; Ward, Sean M.; Sanders, Kenton M.

doi:10.1152/ajpgi.00217.2001

Cited by 17 publications

(14 citation statements)

References 21 publications

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“…The role of ICC in control of colonic motor patterns is still controversial, most information is derived from animal studies [Smith et al, 1987;Pluja et al, 2001;Keef et al, 2002;Alberti et al, 2007;Forrest et al, 2008;Huizinga et al, 2011], and species differences may be significant. Physiological studies of ICC in human colon are only preliminary.…”

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confidence: 99%

Ultrastructure of Interstitial Cells in Subserosa of Human Colon

Rumessen

Vanderwinden

Hansen

et al. 2013

Cells Tissues Organs

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We studied the ultrastructure of interstitial cells in the subserosal/adventitial layer in human colon. An interstitial cell type with an ultrastructure intermediate between fibroblast-like cells (FLC) and interstitial cells of Cajal was identified (IC-SS). IC-SS had thin and flattened branching processes, most densely arranged close to the longitudinal muscle cells. Caveolae, bundles of intermediate filaments and membrane-associated dense bands, often with a patchy basal lamina, were characteristic. Secretory organelles (granular endoplasmic reticulum, smooth endoplasmic reticulum, Golgi, coated vesicles) were prominent. The IC-SS ultrastructure was different from that of FLC in the longitudinal layer, which had no caveolae and fewer intermediate filaments. Peg-and-socket junctions between IC-SS and between IC-SS and muscle cells were present, and IC-SS processes had close, selective appositions to muscle cells. Gap junctions were not observed. Small nerve bundles were abundant, but close contacts (<100 nm) between IC-SS or muscle cells and nerves were inconspicuous. Close appositions between IC-SS and mast cells were present; close relations to macrophages were not observed. The myoid features of IC-SS are thus more pronounced in comparison with FLC of other locations in the gastrointestinal muscle. The organization and ultrastructure may suggest a regulatory nature of IC-SS on the colonic muscle layers.

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mentioning

confidence: 99%

Ultrastructure of Interstitial Cells in Subserosa of Human Colon

Rumessen

Vanderwinden

Hansen

et al. 2013

Cells Tissues Organs

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“…Colonic ICC both at the level of the myenteric (ICC-MP) and at the submuscular plexus (ICC-SMP) may function as pacemaker cells, driving longitudinal and circular muscle contractions [5][6][7][8][9][10]. ICC have previously been studied by light microscopy (c-kit immunohistochemistry) in human colonic muscle both in normal tissue and in a range of gut motility disorders [e.g., 2, [11][12][13][14][15][16][17][18][19][20][21][22][23].…”

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confidence: 99%

Ulcerative Colitis: Ultrastructure of Interstitial Cells in Myenteric Plexus

Rumessen

Vanderwinden

Horn

2010

Ultrastructural Pathology

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Interstitial cells of Cajal (ICC) are key regulatory cells in the gut. In the colon of patients with severe ulcerative colitis (UC), myenteric ICC had myoid ultrastructural features and were in close contact with nerve terminals. In all patients as opposed to controls, some ICC profiles showed degenerative changes, such as lipid droplets and irregular vacuoles. Nerve terminals often appeared swollen and empty. Glial cells, muscle cells, and fibroblast-like cells (FLC) showed no alterations. FLC enclosed macrophages (MLC), which were in close contact with naked axon terminals. The organization and cytological changes may be of pathophysiological significance in patients with UC.

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“…However, the exact cellular origin and electrophysiological characteristics of colonic pacemaker activity is still controversial, perhaps because of species differences (Smith et al 1987;Pluja et al 2001;Keef et al 2002;Alberti et al 2007). Physiological studies of ICC in human colon are preliminary, but they have supported the existence of distinct cellular populations responsible for rhythmic activities and slow waves at the level of the myenteric plexus and at the submuscular level (Rae et al 1998).…”

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confidence: 99%

Ultrastructure of interstitial cells of Cajal in myenteric plexus of human colon

et al. 2009

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The role of the interstitial cells of Cajal (ICC) associated with the myenteric plexus (ICC-MP) as regulators of the motility of the colonic external muscle remains unclear. Ultrastructural studies of myenteric interstitial cells are lacking in human colon. We therefore characterized the distinctive ultrastructure of these cells in the myenteric region of the colon by transmission electron microscopy of the region between the main muscle layers in all parts of the colon in unaffected areas of resected specimens from nine adult human patients. ICC-MP were similar in various colonic regions and had myoid features such as scattered caveolae, prominent intermediate filaments, and cytoplasmic dense bodies. We found characteristic dense membrane-associated bands with a patchy basal lamina, invaginating cellular protrusions (peg and socket junctions) between ICC and between ICC and muscle cells, and close contacts (<100 nm) between ICC and nerves. No gap junctions were observed. Fibroblast-like cells (FLC) were abundant showing well-developed secretory organelles, including coated vesicles, but lacked prominent intermediate filaments and caveolae. FLC had a patchy basal lamina, and peg and socket junctions were observed between them. Macrophage-like cells frequently occurred in close apposition with FLC and, more seldomly, with ICC-MP. The ultrastructure of ICC and FLC in the myenteric region of the human colon thus differs characteristically, but significant overlaps in the ultrastructure between ICC and FLC might complicate any interpretation in pathological ultrastructural studies of the human colonic muscle layer.

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Electrical activity induced by nitric oxide in canine colonic circular muscle

Cited by 17 publications

References 21 publications

Ultrastructure of Interstitial Cells in Subserosa of Human Colon

Ultrastructure of Interstitial Cells in Subserosa of Human Colon

Ulcerative Colitis: Ultrastructure of Interstitial Cells in Myenteric Plexus

Ultrastructure of interstitial cells of Cajal in myenteric plexus of human colon

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