Electrical Activity in an Exocrine Gland: Optical Recording with a Potentiometric Dye

Senseman, David M.; Salzberg, Brian M.

doi:10.1126/science.7375937

Cited by 31 publications

(17 citation statements)

References 22 publications

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“…The response time constant of the light measuring system was 180 gsec. Similar results were obtained with the merocyanine dye, XXII bleaching were more obvious (Grinvald et al, 1981 b;Senseman & Salzberg, 1980).…”

Section: Photodynamic Damage and Dye Bleachingsupporting

confidence: 76%

Improvements in optical methods for measuring rapid changes in membrane potential

et al. 1981

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In an effort to increase the utility of optical methods for measuring membrane potential in excitable cells, an additional 369 dyes were tested on giant axons from the squid. Several promising dyes with relatively large absorption and fluorescence signals are described. In addition, a simple modification of the apparatus led to a sixfold increase in the size of dye-related birefringence signals. In preparations with a suitable geometry, these signals are as large as absorption signals but photodynamic damage and bleaching are eliminated when wavelengths longer than the absorption band are used.

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Section: Photodynamic Damage and Dye Bleachingsupporting

confidence: 76%

Improvements in optical methods for measuring rapid changes in membrane potential

et al. 1981

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“…(The phenomenon is illustrated dramatically in Fig. 2A of the paper by Senseman and Salzberg (1980), which shows a dc coupled photodiode recording.) Here, again, the mechanism is largely unknown, although, in the case of the styryl dyes, the conjugated carbon chain is the probable site of the lesion.…”

Section: Dye Bleachingmentioning

confidence: 97%

Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis

Obaid

Loew

Wuskell

et al. 2004

Journal of Neuroscience Methods

121

108

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“…Based on the results obtained in previous optical studies (e.g. Senseman & Salzberg, 1980;Grinvald et al, 1982;Orbach & Cohen, 1983), it was expected that the VSD signal component recorded at 550 nm will be inverted when compared to that recorded at 720 nm. On the other hand, shortening the illuminating wavelength from 720 to 550 nm should only alter the relative magnitude of the intrinsic signal component, but have little effect on either its "sign" or the shape of its waveform (Cohen & Keynes, 1971).…”

Section: Contamination Of the Vsd Signal By An Intrinsic Optical Compmentioning

confidence: 98%

Correspondence between visually evoked voltage-sensitive dye signals and synaptic activity recorded in cortical pyramidal cells with intracellular microelectrodes

Senseman

1996

Vis Neurosci

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Fast, multiple-site optical recording of voltage-sensitive dye (VSD) signals and intracellular microelectrode recordings were combined to characterize visually evoked neuronal responses in the visual cortex of the pond turtle, Pseudemys scripta. By using an in vitro, eye-brain preparation stained with the merocyanine oxazolone voltage-sensitive dye, NK-2495 or a close analog, NK-2761, large VSD signals relatively free of vibrational noise could be recorded in single trials following a stroboscopic light flash to the contralateral eye. VSD signals recorded from the same cortical location in repeated trials exhibited considerable variability in the onset, duration, and amplitude of secondary depolarizations. Because of this variability, secondary depolarizations were largely absent in signal-averaged responses. Superposition of VSD signals with intracellular recordings obtained from cortical pyramidal cells revealed a close correspondence between their signal waveforms. The two signals were virtually identical in their onset, initial rate of rise, and time-to-peak. At later periods (>500 ms), the correspondence was less close, especially for large cortical depolarizations. Some of this disparity could be attributed to contamination of the VSD signal by a large intrinsic optical response. A second contribution was a failure of the VSD signal to register asynchronous regenerative effects occurring in single pyramidal cells. It is suggested that the close correspondence between the microelectrode and optical recordings in the early phase of the response may reflect the organization of pyramidal cells into clusters that receive virtually identical synaptic inputs.

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Electrical Activity in an Exocrine Gland: Optical Recording with a Potentiometric Dye

Cited by 31 publications

References 22 publications

Improvements in optical methods for measuring rapid changes in membrane potential

Improvements in optical methods for measuring rapid changes in membrane potential

Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis

Correspondence between visually evoked voltage-sensitive dye signals and synaptic activity recorded in cortical pyramidal cells with intracellular microelectrodes

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