ELECTRICAL ACTIVITIES IN THIN SECTIONS FROM THE MAMMALIAN BRAIN MAINTAINED IN CHEMICALLY‐DEFINED MEDIA <i>IN VITRO</i>

Yamamoto, Chosaburo; McIlwain, H.

doi:10.1111/j.1471-4159.1966.tb04296.x

Cited by 383 publications

(99 citation statements)

References 2 publications

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“…These observations were thought to be the biochemical events accompanying neurone discharges, an interpretation that was strengthened with the discovery of post-synaptic activity in slices of piriform cortex (Yamamoto & Mcllwain, 1966 a, b) and unit discharges in slices of mammalian neocortex and prepiriform cortex (Richards & Sercombe, 1967;Richards & McIlwain, 1967). This paper is chiefly concerned with the post-synaptic activity observed in the prepiriform cortex preparation and extends the original observations of Yamamoto & McIlwain (1966b). We believe that preparations of this type offer considerable scope for investigating synaptic transmission in the mammalian brain.…”

mentioning

confidence: 95%

Electrical activity observed in guinea‐pig olfactory cortex maintained in vitro

Richards¹,

Sercombe²

1968

The Journal of Physiology

122

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SUMMARY1. Electrical recordings were made from a preparation of guinea-pig olfactory cortex maintained in vitro.2. The response of the prepiriform area to a volley delivered to the lateral olfactory tract comprised a sharp negative deflexion upon which several positive notches were superimposed followed by a long-duration low-amplitude positive wave.3. Both the negative and positive waves were of maximal amplitude near the top surface of the preparation (the original cortical surface). 4. Units recorded at depths of 250-350 It showed facilitation and latency variation. The latency distribution of these spikes was very similar to that of the positive notches observed on the surface of the slice.5. The surface observed negative waves were rapidly abolished by oxygen lack.6. The negative and positive waves have been tentatively identified as the extracellular manifestations of the excitatory post-synaptic potentials (EPSPs) and inhibitory post-synaptic potentials (IPSPs) occurring in the apical dendrites.7. Some spontaneous activity was observed in a proportion of the preparations studied.8. The evidence presented here strongly supports the contention that portions of the mammalian brain may be maintained in a satisfactory functional condition when isolated from the body, provided that they are maintained in a suitable environment. We believe that such preparations will prove very useful for physiological studies of the mammalian brain.

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confidence: 95%

Electrical activity observed in guinea‐pig olfactory cortex maintained in vitro

Richards¹,

Sercombe²

1968

The Journal of Physiology

122

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“…By contrast, following entorhinal activation of dentate granule cells, the post-augmentation potentiated state only lasted 30 s, possibly because of the barbiturate anaesthesia used (Andersen et al 1966). In their pioneering work on prepiriform cortical slices, Yamamoto & McIlwain (1966) reported that stimulation of the olfactory tract at 100 Hz for 10 s was followed by enhanced N-waves (fEPSPs) for 0.5-3 min, interpreted as PTP.…”

Section: Effects Following the Augmentationmentioning

confidence: 99%

A prelude to long-term potentiation

Andersen

2003

Phil. Trans. R. Soc. Lond. B

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“…In this context, a preparation that would seem to possess several advantages is the isolated, perfused olfactory cortex slice. Thus, electrical stimulation of its excitatory input, the lateral olfactory tract, activates a dense but relatively simple synaptic network and is accompanied by a characteristic series of surface field potentials, the electrical manifestations of synaptic processes within the preparation (Yamamoto & McIlwain, 1966;Richards & Sercombe, 1968;Haberly, 1973a, b;Harvey, Scholfield & Brown, 1974;Pickles & Simmonds, 1976, 1978. The neuroanatomical correlates of some of these processes are known (Stevens, 1969).…”

Section: Introductionmentioning

confidence: 99%

Evidence of a neurotransmitter role for aspartate and γ‐aminobutyric acid in the rat olfactory cortex

Collins

1979

The Journal of Physiology

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SUMMARY1. Using a cortical cup technique, the release of seven endogenous amino acids from the isolated rat olfactory cortex slice has been monitored.2. Electrical stimulation of the lateral olfactory tract at a frequency of 4 min-was accompanied by a significant increase in the release of aspartate, GABA and taurine; the release of GABA and aspartate but not that of taurine was Ca2+-dependent.3. Chronic unilateral bulbectomy was accompanied by a specific, significant fall in the aspartate content of the olfactory cortex which reached a maximum 5 days after surgery and persisted for at least a further 5 days. Electrical stimulation of the lateral olfactory tract of such preparations did not release any of the amino acids under investigation.4. When slices from the unoperated side were exposed to solutions containing 25 mM-K+ or stimulated with electrodes placed directly on the cortex enclosed by the cup there was a Ca2+-dependent release of aspartate and GABA accompanied by a Ca2+-independent release of taurine. Following chronic bulbectomy, these procedures failed to evoke significant release of aspartate whereas the characteristics of GABA and taurine release were unaltered.5. It is concluded that aspartate may be the excitatory transmitter of some of the terminals of the lateral olfactory tract fibres and that GABA may be a transmitter at some inhibitory synapses of the rat olfactory cortex.

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ELECTRICAL ACTIVITIES IN THIN SECTIONS FROM THE MAMMALIAN BRAIN MAINTAINED IN CHEMICALLY‐DEFINED MEDIA IN VITRO

Cited by 383 publications

References 2 publications

Electrical activity observed in guinea‐pig olfactory cortex maintained in vitro

Electrical activity observed in guinea‐pig olfactory cortex maintained in vitro

A prelude to long-term potentiation

Evidence of a neurotransmitter role for aspartate and γ‐aminobutyric acid in the rat olfactory cortex

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