Electrical activation of oocytes after intracytoplasmic sperm injection: a controlled randomized study

Mansour, Ragaa; Fahmy, Ibrahim; Tawab, Nevine A.; Kamal, Ahmed; El-Demery, Yehia; Aboulghar, Mohamed; Serour, Gamal I.

doi:10.1016/j.fertnstert.2007.08.017

Cited by 79 publications

(45 citation statements)

References 47 publications

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“…To rescue cases that encounter problems in oocyte activation immediately following sperm-oocyte fusion, certain artificial activation procedures have been recommended: mechanical activation by a modified ICSI technique [8,9], electrical activation [10,11], chemical compounds such as strontium chloride [12,13], ionomycin [14], and calcium ionophores [15], and sequential treatment with calcium ionophore and puromycin [16].…”

Section: Introductionmentioning

confidence: 99%

Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore

Yoon

Bae

Kim

et al. 2013

J Assist Reprod Genet

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Purpose Fertilization failures have occurred repeatedly in reproductive centers after intracytoplasmic sperm injection (ICSI) and artificial oocyte activation (AOA) has been used to prevent it. This study was performed to investigate whether spermatozoan origin influences clinical outcomes of AOAwith a calcium ionophore. Methods A total of 185 ICSI cycles with a history of no or low fertilization was included in this retrospective study. The outcomes of AOA after ICSI were compared with ejaculatednormal, ejaculated-oligo-astheno-terato or extracted-testicular spermatozoa. Results There were significant differences between the previous standard ICSI cycles and AOA cycles in the rate of fertilization and clinical outcomes among cases with different sperm origins. Thirty-eight healthy babies (20 singles and 18 twins, 29 cycles) were successfully delivered, and no congenital birth defects were observed. Conclusions Most patients with a no or low fertilization history obtained an increased fertilization rate and a positive clinical outcome with AOA regardless of the origin of spermatozoa.

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Section: Introductionmentioning

confidence: 99%

Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore

Yoon

Bae

Kim

et al. 2013

J Assist Reprod Genet

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“…In a previous study of Mansour et al, the fertilization rates were 68% in electro activated patients with severe oligoasthenoteratozoospermia (OAT) and azoospermia whereas the 60% in control group [12]. We also intended to evaluate the effects of piezoelectric stimulation on severe oligozoospermia patients' IVF cycles and couldn't observe any significant difference as our fertilization rate was 64%.…”

Section: Groupsmentioning

confidence: 94%

“…While designing our studies, we preferred the nonchemical activation method such as electrical stimulation [12,24,26] as chemical activation was shown to induce parthenogenetic development of oocytes [47]. On the other hand about 70%-80% of unfertilized oocytes after ICSI responded to electro activation and formed two pronuclei [24,36].…”

Section: Groupsmentioning

confidence: 99%

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The Effect of Piezoelectric Stimulation in Patients with Low Fertilization Potential

Ünsal¹

2014

Human Genet Embryol

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Objective: To assess the value of the electrical activation of oocytes in ICSI patients with previous limited fertilization outcomes.Design: Prospective randomized study. Settings Result(s):Patients were subdivided into six study groups. In Group I, testicular elongated spermatids were used and 27.9% fertilization, 2.3% clinical pregnancy rates were evaluated. In testicular immotile spermatozoa injected group (Group II), 56.5% fertilization and 50% clinical pregnancy rates were obtained. 66.7% fertilization and 30%clinical pregnancy rates were achieved in Group III with testicular motile spermatozoa. In Group IV, patients with severe oligozoospermia, 64% fertilization and 28.6% clinical pregnancy rates were achieved. Group V included patients with total immotile spermatozoa and fertilization and clinical pregnancy rates were 50% and 57.1% respectively. In patients with history of low fertilization rate (Group VI), 38.7% fertilization and 19% clinical pregnancy rates were obtained. Conclusion(s):Piezoelectric stimulation can be used for patients with low fertilization rates and total immotile spermatozoa; as we detected an improvement in the fertilization and clinical pregnancy rates of these patients.

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“…If sperm is not present an incomplete parthenogenetic development can be initiated, undergoing some abortive cleavages in the best of cases, but without reaching further development (CardonaCosta et al, 2009), or even the configuration of four well organized cells (Lee et al, 1999). In mammals, oocyte activation is routinely induced or reinforced by electrical sequences associated to somatic cloning by nuclear transplant, NT (Onishi et al, 2000;Okahara-Narita et al, 2007), intracytoplasmic sperm injection, ICSI (Zhang et al, 1997;Mansour et al, 2009), or in the obtaining of parthenogenetic haploid/diploid embryos (Elsheikh et al, 1995;Escribá and García-Ximénez, 2000). These set of techniques are nowadays of marked importance in regenerative medicine and/or assisted reproduction in vertebrate models and even, in humans (ICSI).…”

Section: Resumen Electroactivación De Oocitos De Pez Cebra (Danio Rerio)mentioning

confidence: 99%

Electroactivation of zebrafish (Danio rerio) eggs

Cardona-Costa

Pérez-Camps

García-Ximénez

1970

Span. j. agric. res.

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The zebrafish, has recently acquired a complex role in many emerging areas of knowledge, mainly in biomedical research (Nüsslein-Volhard and Dahm, 2002), becoming a new scientific tool in multitude of labs around the world. In this sense, several and varied are the topical areas where the zebrafish has established as a both efficient and capable vertebrate model for developing knowledge. As Streisinger (1986) pointed out, the reasons were their many useful biological characteristics.In zebrafish, egg activation takes place after they are discharged from the ovarian stroma and come into AbstractIn zebrafish, initial egg activation by water after being discharged from the ovarian stroma is followed by normal cleavages only in inseminated eggs. When sperm (genetically active or not) is not used as activating agent, reproductive techniques (as either nuclear transplant or intracytoplasmic sperm injection are ineff icient. In this work, three experiments of egg activation by electric stimuli were performed: In the first, six activation treatments were compared (Voltage × Pulses: 2.76 × 1; 2.76 × 2; 2.76 × 3 and 5.40 × 1; 5.40 × 2; 5.40 × 3). The group 5.40 × 3 showed the best results (32% activated). In the second experiment, an electrical treatment of 20 min was carried out. It consisted in a sequence of three equal electrical stimuli every 10 min (of 1 or 3 consecutive direct current square pulses for 20 µs each and applied at two voltage levels, 2.76 V or 5.4 V). It was observed that the number of pulses negatively affected the rates of damaged and lysed eggs. Moreover, only the 20 min treatment with the combination of 3 consecutive pulses at 2.76 V showed significant differences with their respective control group (43% vs 18% activated eggs, p < 0.05). In the third experiment, negative effects of egg ageing were observed. In conclusion, the best activation treatment for intact (non manipulated) zebrafish eggs concerns sequence B3. The electro-activation stimulus proposed here would be the only one available once the egg has already been activated by water.Additional key words: egg activation; fish; nuclear transplant; oocyte. Resumen Electroactivación de oocitos de pez cebra (Danio rerio)En pez cebra, una vez que los oocitos son descargados del estroma ovárico, su activación inicial por contacto en agua da lugar a divisiones normales únicamente cuando son fertilizados. Cuando no se utiliza semen (inactivado genéticamente o no), las técnicas reproductivas (como transplante nuclear o inyección espermática intracitoplasmática) son ineficientes. En el presente trabajo se desarrollaron tres experimentos de activación oocitaria por estímulos eléctricos: en el primero de ellos, se compararon seis tratamientos de activación (Voltaje × Pulsos: 2,76 × × 1; 2,76 × 2; 2,76 × 3 y 5,40 × 1; 5,40 × 2; 5,40 × 3). El grupo 5,4 × 3 mostró los mejores resultados (32% de activación). En el segundo experimento, se evaluó un tratamiento eléctrico de 20 min de duración. El tratamiento consistió en la aplicación de una secuencia de tre...

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Electrical activation of oocytes after intracytoplasmic sperm injection: a controlled randomized study

Cited by 79 publications

References 47 publications

Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore

Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore

The Effect of Piezoelectric Stimulation in Patients with Low Fertilization Potential

Electroactivation of zebrafish (Danio rerio) eggs

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