Electric Stimulation of Astaxanthin Biosynthesis in Haematococcus pluvialis

Fitriana, Hana-Nur; Lee, Soo-Youn; Choi, Suna; Lee, Ji Ye; Kim, Bolam; Lee, Jin-Suk; Oh, You-Kwan

doi:10.3390/app11083348

Cited by 15 publications

(14 citation statements)

References 30 publications

(53 reference statements)

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“…This could be an interesting topic for further investigation. The optical appearance of H. pluvialis cells during the photosynthetic life cycle (Figure 2b) can be divided into five different cell types, such as red cyst cells, germinating cells, biflagellate cells, green palmella cells, and brownish-green/brown palmella cells, similar to previous observations [1,12]. When inoculated into fresh medium, red cyst cells started to germinate rapidly.…”

Section: Growth and Morphology Of H Pluvailis With Rac-gr24supporting

confidence: 85%

“…Astaxanthin biomolecules accumulate inside neutral lipid bodies forming ester bonds with long-chain fatty acids, enabled by closely interconnected metabolic networks of lipogenesis and carotenogenesis [6]. To improve the astaxanthin productivity of photosynthetic H. pluvialis cultures, various stress-inducing strategies, such as nitrogen starvation [7], strong light irradiance [8], high salinity [9], high temperature [10], mechanical stress [11], electrical treatment [12], and aminoclay nanoparticle addition [2] have been applied. However, these approaches are time-consuming, require high energy input and large amounts of chemicals; additionally, they may substantially inhibit cell growth.…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of Astaxanthin and Fatty Acid Production in Haematococcus pluvialis Using Strigolactone

et al. 2022

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Improving the production rate of high-value nutraceutical compounds, such as astaxanthin and polyunsaturated fatty acids (PUFAs), is important for the commercialization of Haematococcus pluvialis biorefineries. Here, the effects of a phytohormone, strigolactone analog rac-GR24, on cell growth and astaxanthin and fatty acid biosynthesis in H. pluvialis were investigated. Four concentrations (2, 4, 6, and 8 µM) of rac-GR24 were initially added during 30 days of photoautotrophic cultivation. The addition of rac-GR24 improved cell number density and chlorophyll concentration in H. pluvialis cultures compared to the control; the optimal concentration was 8 µM. Despite a slightly reduced astaxanthin content of 30-d-old cyst cells, the astaxanthin production (26.1 ± 1.7 mg/L) improved by 21% compared to the rac-GR24-free control (21.6 ± 1.5 mg/L), owing to improved biomass production. Notably, at the highest dosage of 8 µM rac-GR24, the total fatty acid content of the treated H. pluvialis cells (899.8 pg/cell) was higher than that of the untreated cells (762.5 pg/cell), resulting in a significant increase in the total fatty acid production (361.6 ± 48.0 mg/L; 61% improvement over the control). The ratio of PUFAs, such as linoleic (C18:2) and linolenic (C18:3) acids, among total fatty acids was high (41.5–44.6% w/w) regardless of the rac-GR24 dose.

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Section: Growth and Morphology Of H Pluvailis With Rac-gr24supporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Enhancement of Astaxanthin and Fatty Acid Production in Haematococcus pluvialis Using Strigolactone

et al. 2022

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“…Kim et al [24] subjected biflagellate cells of H. lacustris to a strong current intensity (100 mA) for a brief duration (1 min), resulting in a considerable increase (20%) in cell number density, although this treatment did not cause alteration in the AXT content compared to that of untreated controls after subsequent photosynthetic cultivation for seven days. Conversely, Fitriana et al [25] continuously electrotreated H. lacustris biflagellate cells at a relatively low intensity (30 mA) for an extended period (two days), resulting in a 36.9% higher AXT content (6.0 mg/g cell) than that of the untreated cells. Aerobic electrochemical conditions [26] can generate reactive oxygen species (ROS), which act as signaling molecules to help microalgal cells adapt to stress and activate AXT biosynthesis [3].…”

Section: Introductionmentioning

confidence: 99%

“…Aerobic electrochemical conditions [26] can generate reactive oxygen species (ROS), which act as signaling molecules to help microalgal cells adapt to stress and activate AXT biosynthesis [3]. However, severe cell damage occurs in electrotreated cells, including cellwall and cytoplasm separation along with the extracellular secretion of biomaterials, which is likely due to the excess generation of oxidizing radicals triggered by electrotreatment and a significant drop in pH through water electrolysis [25].…”

Section: Introductionmentioning

confidence: 99%

Rapid Induction of Astaxanthin in Haematococcus lacustris by Mild Electric Stimulation

Sathiyavahisan,

Lakshmi Narasimhan,

Mahadi

et al. 2023

Applied Sciences

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Efficient induction of astaxanthin (AXT) biosynthesis remains a considerable challenge for the industrialization of the biorefinement of the microalga Haematococcus lacustris. In this study, we evaluated the technical feasibility of photosynthetic electrotreatment to enhance AXT accumulation in H. lacustris. The AXT content of H. lacustris electrotreated at an optimal current intensity (10 mA for 4 h) was 21.8% to 34.9% higher than that of the untreated control group, depending on the physiological state of the initial palmella cells. The contents of other carotenoids (i.e., canthaxanthin, zeaxanthin, and β-carotene) were also increased by this electrotreatment. However, when H. lacustris cells were exposed to more intense electric treatments, particularly at 20 and 30 mA, cell viability significantly decreased to 84.2% and 65.6%, respectively, with a concurrent reduction in the contents of both AXT and the three other carotenoids compared to those of the control group. The cumulative effect of electric stimulation is likely related to two opposing functions of reactive oxygen species, which facilitate AXT biosynthesis as signaling molecules while also causing cellular damage as oxidizing radicals. Collectively, our findings indicate that when adequately controlled, electric stimulation can be an effective and eco-friendly strategy for inducing targeted carotenoid pigments in photosynthetic microalgae.

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“…Astaxanthin (3,3 -dihydroxy-β,β -carotene-4,4 -dione), a secondary ketocarotenoid phytopigment, is widely used in the cosmetic, aquaculture, food, and pharmaceutical industries [1,2]. The potent antioxidant activity of astaxanthin has preventive effects against cancer, diabetes, cardiovascular disease, ulcers, and inflammation through the enhancement of the immune response [3,4].…”

Section: Introductionmentioning

confidence: 99%

Room-Temperature Cell Disruption and Astaxanthin Recovery from Haematococcus lacustris Cysts Using Ultrathin α-Quartz Nanoplates and Ionic Liquids

et al. 2022

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Ionic liquids (ILs) are new green solvents, which are widely used in lignocellulosic and microalgal biorefineries. However, high-temperature operating conditions limit their application in the extraction of heat-labile algal products, such as bioactive astaxanthin. In this study, we report the technical feasibility of room-temperature astaxanthin extraction from Haematococcus lacustris cysts with a thick and complex cell wall structure, by combining ultrathin α-quartz nanoplates (NPLs) with ethyl-3-methylimidazolium ([Emim])-based ILs. When four different [Emim]-based ILs with thiocyanate (SCN), diethylphosphate (DEP), HSO4, and Cl anions were applied to 90-day-old H. lacustris cysts at room temperature (~28 °C), the astaxanthin extraction efficiency was as low as 9.6-14.2%. Under sonication, α-quartz NPLs disrupted the cyst cell wall for a short duration (5 min). The astaxanthin extraction efficacies of a subsequent IL treatment improved significantly to 49.8% for [Emim] SCN, 60.0% for [Emim] DEP, 80.7% for [Emim] HSO4, and 74.3% for [Emim] Cl ions, which were 4.4, 6.1, 8.4, and 5.2 times higher than the extraction efficacy of only ILs, respectively. This finding suggests that α-quartz NPLs can serve as powerful cell-wall-disrupting agents for the room-temperature IL-mediated extraction of astaxanthin from robust algal cyst cells.

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Electric Stimulation of Astaxanthin Biosynthesis in Haematococcus pluvialis

Cited by 15 publications

References 30 publications

Enhancement of Astaxanthin and Fatty Acid Production in Haematococcus pluvialis Using Strigolactone

Enhancement of Astaxanthin and Fatty Acid Production in Haematococcus pluvialis Using Strigolactone

Rapid Induction of Astaxanthin in Haematococcus lacustris by Mild Electric Stimulation

Room-Temperature Cell Disruption and Astaxanthin Recovery from Haematococcus lacustris Cysts Using Ultrathin α-Quartz Nanoplates and Ionic Liquids

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