Electric signals regulate directional migration of ventral midbrain derived dopaminergic neural progenitor cells via Wnt/GSK3β signaling

Liu, Jia; Zhu, Bangfu; Zhang, Gaofeng; Wang, Jian; Tian, Weiming; Ju, Gong; Wei, Xiao-Qing; Song, Bing

doi:10.1016/j.expneurol.2014.09.014

Cited by 29 publications

(39 citation statements)

References 31 publications

(31 reference statements)

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“…Taking a bioengineering approach, several works have reported that electric fields can be used to stimulate and direct the migration (termed galvanotaxis) of neural stem cells in vitro or ex vivo [13–17]. These experiments are based upon the understanding that endogenous electrical signals are present in many developing systems [18], and that crucial cellular behaviors are under the influence of such endogenous electric cues including: cell division, migration, and differentiation.…”

Section: Introductionmentioning

confidence: 99%

Specific Intensity Direct Current (DC) Electric Field Improves Neural Stem Cell Migration and Enhances Differentiation towards βIII-Tubulin+ Neurons

et al. 2015

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Control of stem cell migration and differentiation is vital for efficient stem cell therapy. Literature reporting electric field–guided migration and differentiation is emerging. However, it is unknown if a field that causes cell migration is also capable of guiding cell differentiation—and the mechanisms for these processes remain unclear. Here, we report that a 115 V/m direct current (DC) electric field can induce directional migration of neural precursor cells (NPCs). Whole cell patching revealed that the cell membrane depolarized in the electric field, and buffering of extracellular calcium via EGTA prevented cell migration under these conditions. Immunocytochemical staining indicated that the same electric intensity could also be used to enhance differentiation and increase the percentage of cell differentiation into neurons, but not astrocytes and oligodendrocytes. The results indicate that DC electric field of this specific intensity is capable of promoting cell directional migration and orchestrating functional differentiation, suggestively mediated by calcium influx during DC field exposure.

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Section: Introductionmentioning

confidence: 99%

Specific Intensity Direct Current (DC) Electric Field Improves Neural Stem Cell Migration and Enhances Differentiation towards βIII-Tubulin+ Neurons

et al. 2015

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“…EF can be delivered to cells by direct contact of electrodes with cells and the culture medium [38][39][40][41][42] or through a noncontact approach, which isolates electrodes from cells and the culture medium and capacitively couples EF to cells and the culture medium as reported by us [3] and others [43][44][45][46]. Overall, experimental results suggest that different combinations of EF intensity, frequency and/or polarization with respect to the cell, as well as methods of EF delivery, can elicit a variety of distinct cell responses, making mechanistic studies of the EF-cell interactions quite difficult.…”

Section: Introductionmentioning

confidence: 99%

Modulation of cell function by electric field: a high-resolution analysis

Taghian

Narmoneva

Kogan

2015

J. R. Soc. Interface.

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Regulation of cell function by a non-thermal, physiological-level electromagnetic field has potential for vascular tissue healing therapies and advancing hybrid bioelectronic technology. We have recently demonstrated that a physiological electric field (EF) applied wirelessly can regulate intracellular signalling and cell function in a frequency-dependent manner. However, the mechanism for such regulation is not well understood. Here, we present a systematic numerical study of a cell-field interaction following cell exposure to the external EF. We use a realistic experimental environment that also recapitulates the absence of a direct electric contact between the field-sourcing electrodes and the cells or the culture medium. We identify characteristic regimes and present their classification with respect to frequency, location, and the electrical properties of the model components. The results show a striking difference in the frequency dependence of EF penetration and cell response between cells suspended in an electrolyte and cells attached to a substrate. The EF structure in the cell is strongly inhomogeneous and is sensitive to the physical properties of the cell and its environment. These findings provide insight into the mechanisms for frequency-dependent cell responses to EF that regulate cell function, which may have important implications for EF-based therapies and biotechnology development.

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“…Classical models of leading-edge protrusion depend upon a dendritic actin network that undergoes continuous reassembly in order to form ruffling lamellipodia [25,26]. We have previously demonstrated that specific signaling molecules, such as PIP3 and CLASP2, will tend to accumulate along the leading edges of migrating neural stem/progenitor cells along with various cytoskeletal components—including actin [20,21]. In this study, F-actin was found to be asymmetrically redistributed to favor the leading edges of OPCs migrating in an EF (Figure 3b,e).…”

Section: Resultsmentioning

confidence: 99%

“…Often attending the acquisition of viable cells are relevant investigations into cellular motility and signal-driven migration. Consequently, several methods have utilized either biochemical or biophysical means in an attempt to regulate stem cell migration [21,22,27,28,29]. Electric fields have the capacity to function as guidance cues to regulate the migration of both endogenous and grafted neural stem cells [14,29].…”

Section: Discussionmentioning

confidence: 99%

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Electric Signals Regulate the Directional Migration of Oligodendrocyte Progenitor Cells (OPCs) via β1 Integrin

Zhu

Nicholls

et al. 2016

IJMS

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The guided migration of neural cells is essential for repair in the central nervous system (CNS). Oligodendrocyte progenitor cells (OPCs) will normally migrate towards an injury site to re-sheath demyelinated axons; however the mechanisms underlying this process are not well understood. Endogenous electric fields (EFs) are known to influence cell migration in vivo, and have been utilised in this study to direct the migration of OPCs isolated from neonatal Sprague-Dawley rats. The OPCs were exposed to physiological levels of electrical stimulation, and displayed a marked electrotactic response that was dependent on β1 integrin, one of the key subunits of integrin receptors. We also observed that F-actin, an important component of the cytoskeleton, was re-distributed towards the leading edge of the migrating cells, and that this asymmetric rearrangement was associated with β1 integrin function.

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Electric signals regulate directional migration of ventral midbrain derived dopaminergic neural progenitor cells via Wnt/GSK3β signaling

Cited by 29 publications

References 31 publications

Specific Intensity Direct Current (DC) Electric Field Improves Neural Stem Cell Migration and Enhances Differentiation towards βIII-Tubulin+ Neurons

Specific Intensity Direct Current (DC) Electric Field Improves Neural Stem Cell Migration and Enhances Differentiation towards βIII-Tubulin+ Neurons

Modulation of cell function by electric field: a high-resolution analysis

Electric Signals Regulate the Directional Migration of Oligodendrocyte Progenitor Cells (OPCs) via β1 Integrin

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