Electric Phenomenon: A Disregarded Tool in Tissue Engineering and Regenerative Medicine

Silva, Lucília P. da; Kundu, Subhas C.; Reis, Rui L.; Correlo, Vítor M.

doi:10.1016/j.tibtech.2019.07.002

Cited by 97 publications

(99 citation statements)

References 133 publications

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“…Owing to biocompatibility, biodegradability, and nontoxicity [13,14] PHB is used in different medical, packaging, and environmental applications, although its wide application is limited by current high cost. In fact, PLA, PHB, and their blends can be considered favorably for biomedical applications [15,16] especially in the tissue engineering, regenerative medicine and as the biodegradable therapeutic systems for drug delivery [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Biodegradable Polylactide–Poly(3-Hydroxybutyrate) Compositions Obtained via Blending under Shear Deformations and Electrospinning: Characterization and Environmental Application

et al. 2020

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Compositions of polylactide (PLA) and poly(3-hydroxybutyrate) (PHB) thermoplastic polyesters originated from the nature raw have been obtained by blending under shear deformations and electrospinning methods in the form of films and nanofibers as well as unwoven nanofibrous materials, respectively. The degrees of crystallinity calculated on the base of melting enthalpies and thermal transition temperatures for glassy state, cold crystallization, and melting point for individual biopolymers and ternary polymer blends PLA-PHB- poly(ethyleneglycol) (PEG) have been evaluated. It has been shown that the mechanical properties of compositions depend on the presence of plasticizers PEG with different molar masses in interval of 400–1000. The experiments on the action of mold fungi on the films have shown that PHB is a fully biodegradable polymer unlike PLA, whereas the biodegradability of the obtained composites is determined by their composition. The sorption activity of PLA–PHB nanofibers and unwoven nanofibrous PLA–PHB composites relative to water and oil has been studied and the possibility of their use as absorbents in wastewater treatment from petroleum products has been demonstrated.

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Section: Introductionmentioning

confidence: 99%

Biodegradable Polylactide–Poly(3-Hydroxybutyrate) Compositions Obtained via Blending under Shear Deformations and Electrospinning: Characterization and Environmental Application

et al. 2020

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“…While this phenomenon is commonly observed among many cell types 51,57-60 , the molecular mechanism and the biological role are not clear. Electroalignment may participate in the cytoskeletal restructuring in tissue morphogenesis 61,62 , but biophysical studies show that in vitro microtubules align in parallel to electric field vectors rather than perpendicular 63,64 . Supplementary FIG.…”

Section: Electrotaxis Behavior May Reflect the Heterogeneity Of Glmentioning

confidence: 99%

Voltage-gated ion channels mediate the electrotaxis of glioblastoma cells in a hybrid PMMA/PDMS microdevice

Tsai

IJspeert

Shen

2020

Preprint

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Transformed astrocytes in the most aggressive form cause glioblastoma, the most common cancer in central nervous system with high mortality. The physiological electric field by neuronal local field potentials and tissue polarity may guide the infiltration of glioblastoma cells through the electrotaxis process. However, microenvironments with multiplex gradients are difficult to create. In this work, we have developed a hybrid microfluidic platform to study glioblastoma electrotaxis in controlled microenvironments with high throughput quantitative analysis by a machine learning-powered single cell tracking software. By equalizing the hydrostatic pressure difference between inlets and outlets of the microchannel, uniform single cells can be seeded reliably inside the microdevice. The electrotaxis of two glioblastoma models, T98G and U-251MG, require optimal laminin-containing extracellular matrix and exhibits opposite directional and electro-alignment tendencies. Calcium signaling is a key contributor in glioblastoma pathophysiology but its role in glioblastoma electrotaxis is still an open question. Anodal T98G electrotaxis and cathodal U-251MG electrotaxis require the presence of extracellular calcium cations. U-251MG electrotaxis is dependent on the P/Q-type voltage-gated calcium channel (VGCC) and T98G is dependent on the R-type VGCC. U-251MG and T98G electrotaxis are also mediated by A-type (rapidly inactivating) voltage-gated potassium channels and acidsensing sodium channels. The involvement of multiple ion channels suggests that the glioblastoma electrotaxis is complex and patient-specific ion channel expression can be critical to develop personalized therapeutics to fight against cancer metastasis. The hybrid microfluidic design and machine learning-powered single cell analysis provide a simple and flexible platform for quantitative investigation of complicated biological systems.

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“…As the biophysical cues, solid functional data implicate steady ion currents in cells enhances neural differentiation, neurite extension and growth. [18] Accordingly, extra electrical stimulation induced by electrical fields [19] or bioelectricity meditated by conductive matrix have been utilized to enhance rapid neuronal regeneration. [20] Whereas, for wounds originating from injury, burns, and diabetic skin ulcers, the levels of chemoattractants, neurotrophic factor gene expression, and electrical signaling are usually low or even absent, resulting in poor nerve formation and delayed healing.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Self‐Adaptive All‐In‐One Delivery Chip for Rapid Skin Nerves Regeneration by Endogenous Mesenchymal Stem Cells

Peng

Huang

et al. 2020

Adv Funct Materials

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Skin wound therapy aims not only to restore skin protection but also to recover excitation functions through nerve regeneration. During the restoration of skin nerves, the recruitment of endogenous stem cells and promotion of neuronal regeneration on site work stepwise are foundations of in situ regeneration. However, current therapeutic systems usually execute each process separately, leading to limited regeneration and recovery of excitation functions. Herein, a novel self-adaptive all-in-one delivery chip (G:P:Al-Chip) is constructed that combines therapeutic protein release, gene delivery, and electrical conduction in a single microfluidic chip by 3D coaxial printing. G:P:Al-Chip consists of an outer conductive hydrogel shell anchored with chemokine and an inner microchannel filled with enzyme-initiated vector/ plasmid DNAs microcomplexes. G:P:Al-Chip delivers chemokine, functional plasmid DNAs, and promotes electrical conduction with a self-adaptive program that significantly enhances the recruitment of endogenous mesenchymal stem cells and promotes neuronal regeneration. G:P:Al-Chip is shown to enhance nerve regeneration with excitation functions within 23 days. G:P:Al-Chip organizes recruitment and neuronal regeneration cues along with bioelectrical signal in one degradable chip for accelerated skin nerve regeneration.

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Electric Phenomenon: A Disregarded Tool in Tissue Engineering and Regenerative Medicine

Cited by 97 publications

References 133 publications

Biodegradable Polylactide–Poly(3-Hydroxybutyrate) Compositions Obtained via Blending under Shear Deformations and Electrospinning: Characterization and Environmental Application

Biodegradable Polylactide–Poly(3-Hydroxybutyrate) Compositions Obtained via Blending under Shear Deformations and Electrospinning: Characterization and Environmental Application

Voltage-gated ion channels mediate the electrotaxis of glioblastoma cells in a hybrid PMMA/PDMS microdevice

Self‐Adaptive All‐In‐One Delivery Chip for Rapid Skin Nerves Regeneration by Endogenous Mesenchymal Stem Cells

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