Electric Field Effects on Insulin Chain-B Conformation

Budi, Akin; Legge, Fiona S.; Treutlein, Herbert; Yarovsky, Irene

doi:10.1021/jp052742q

Cited by 93 publications

(157 citation statements)

References 44 publications

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“…9 Although such field intensities are several orders of magnitude larger than those applied experimentally or industrially, 5 it is necessary to apply field intensities greater than circa 0.01 V Å −1 to observe tangible effects for solvated proteins within the limited time scales amenable to molecular simulation. 9,10 Our results are similar to those of the group of Yarovsky, 11 where both static and oscillating (from 1.225 to 4.9 GHz) electric fields were applied to insulin chain-B, and the insulin chain-A-chain-B complex. a) Current address: Department of Biophysical Chemistry, Lund University, Sweden.…”

Section: Introductionsupporting

confidence: 87%

Hydrogen bond perturbation in hen egg white lysozyme by external electromagnetic fields: A nonequilibrium molecular dynamics study

Solomentsev

English

Mooney

2010

The Journal of Chemical Physics

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Nonequilibrium molecular dynamics simulations of a charge-neutral mutant of hen egg white lysozyme have been performed at 300 K and 1 bar in the presence of external microwave fields (2.45 to 100 GHz) of an rms electric field intensity of 0.05 V Å(-1). A systematic study was carried out of the distributions of persistence times and energies of each intraprotein hydrogen bond in between breakage and reformation, in addition to overall persistence over 20 ns simulations, vis-á-vis equilibrium, zero-field conditions. It was found that localized translational motion for formally charged residues led to greater disruption of associated hydrogen bonds, although induced rotational motion of strongly dipolar residues also led to a degree of hydrogen bond perturbation. These effects were most apparent in the solvent exposed exterior of hen egg white lysozyme, in which the intraprotein hydrogen bonds tend to be weaker.

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Section: Introductionsupporting

confidence: 87%

Hydrogen bond perturbation in hen egg white lysozyme by external electromagnetic fields: A nonequilibrium molecular dynamics study

Solomentsev

English

Mooney

2010

The Journal of Chemical Physics

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“…The direct action of an oscillating electric field on a protein in solution has been simulated by Budi et al [2005] using force field molecular dynamics. The electric field can cause unwinding of peripheral regions of the protein backbone and thereby promote unfolding.…”

Section: Discussionmentioning

confidence: 99%

Non‐Thermal effects in the microwave induced unfolding of proteins observed by chaperone binding

George

Bilek

McKenzie

2008

Bioelectromagnetics

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We study the effect of microwaves at 2,450 MHz on protein unfolding using surface plasmon resonance sensing. Our experimental method makes use of the fact that unfolding proteins tend to bind to chaperones on their unfolding pathway and this attachment is readily monitored by surface plasmon resonance. We use the protein citrate synthase (CS) for this study as it shows strong binding to the chaperone alpha crystallin when stressed by exposure to excess temperature. The results of microwave heating are compared with the effect of ambient heating and a combination of ambient and microwave heating to the same final temperature. We study the temperature distributions during the heating process. We show that microwaves cause a significantly higher degree of unfolding than conventional thermal stress for protein solutions heated to the same maximum temperature.

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“…It has also been shown that electromagnetic radiation influences the glucose concentration, dielectric and electrical properties of the blood [29,30]. Electric field effect on insulin chain-B under static and oscillating conditions showed that the electric field caused a destabilizing effect on the peptide [31][32][33]. However, not much work has been done on the effects of cellular phone radiation at 850 MHz on the diabetic blood cells.…”

Section: Introductionmentioning

confidence: 99%

Case Study of High Blood Glucose Concentration Effects of 850 MHZ Electromagnetic Fields Using Gtem Cell

Boriraksantikul¹,

Bhattacharyya²,

Whiteside³

et al. 2012

PIER B

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Abstract-The effect of 850 MHz electromagnetic radiation on diabetic blood at 2 W and 60 W power levels was investigated and compared with normal blood cells. The power levels respectively represent radiations from a cell phone and the cell phone tower, both operating 850 MHz. A GTEM cell was designed for the tests to generate the desired uniform electromagnetic field and power in a shielded environment. Blood samples, having normal and high glucose concentrations, were placed in the usable area inside the GTEM cell for 10, 30, 60 minutes and the glucose levels and red and white blood cell viabilities were monitored and compared with the controls. Results show that the 850 MHz exposure significantly influences the blood cell counts and the glucose level in both normal and high glucose blood samples. In cell survivability analysis in normal blood samples it was found that the white blood cells are significantly higher than the control at 60 min exposure from cell phone radiation, while both the white and red blood cell are significantly higher following a 30 min exposure from tower radiation. For high glucose blood tests at 30 and 60 min exposure times, the tower radiation for 60 min and the cell phone radiation at both the exposure times show significantly changes in white blood cell counts, whereas there was no effect in red blood cells. Also, for 30 and 60 min exposure times, the glucose

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Electric Field Effects on Insulin Chain-B Conformation

Cited by 93 publications

References 44 publications

Hydrogen bond perturbation in hen egg white lysozyme by external electromagnetic fields: A nonequilibrium molecular dynamics study

Hydrogen bond perturbation in hen egg white lysozyme by external electromagnetic fields: A nonequilibrium molecular dynamics study

Non‐Thermal effects in the microwave induced unfolding of proteins observed by chaperone binding

Case Study of High Blood Glucose Concentration Effects of 850 MHZ Electromagnetic Fields Using Gtem Cell

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