ElastoPHB® Membrane Systems with Immobilized Bone Marrow Stromal Cells Optimize Conditions for Regeneration of Damaged Tissue

Потапов, И. В.; Ilinskii, O M; Куренкова, Л. Г.; Sevast'yanov, V. I.; Egorova, Varvara Alekseevna; Зайденов, В. А.; Расулов, М Ф; Онищенко, Н. А.

doi:10.1007/s10517-005-0429-5

Cited by 2 publications

(2 citation statements)

References 17 publications

(16 reference statements)

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“…Shumakov Federal Research Center for Transplantology and Artificial Organs of Rosmedtekhnologii. The films were found to be suitable scaffolds for culturing MSCs and epidermal cells for various applications, including skin wound repair [59]. Similarly, polymer films of P(3HB/3HV) blended with polyethylene glycol (ElastoPOB) carrying bone marrow mesenchymal stem cells were studied.…”

Section: Discussionmentioning

confidence: 99%

Experimental wound dressings of degradable PHA for skin defect repair

Shishatskaya

Nikolaeva

Vinogradova

et al. 2016

J Mater Sci: Mater Med

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The present study reports construction of wound dressing materials from degradable natural polymers such as hydroxy derivatives of carboxylic acids (PHAs) and 3-hydroxybutyrate/4-hydroxybutyrate [P(3HB/4HB)] as copolymer. The developed polymer films and electrospun membranes were evaluated for its wound healing properties with Grafts-elastic nonwoven membranes carrying fibroblast cells derived from adipose tissue multipotent mesenchymal stem cells. The efficacy of nonwoven membranes of P(3HB/4HB) carrying the culture of allogenic fibroblasts was assessed against model skin defects in Wistar rats. The morphological, histological and molecular studies revealed the presence of fibroblasts on dressing materials which facilitated wound healing, vascularization and regeneration. Further it was also observed that cells secreted extracellular matrix proteins which formed a layer on the surface of membranes and promoted the migration of epidermal cells from the neighboring tissues surrounding the wound. The wounds under the P(3HB/4HB) membrane carrying cells healed 1.4 times faster than the wounds under the cell-free membrane and 3.5 times faster than the wounds healing under the eschar (control).The complete wound healing process was achieved at Day 14. Thus the study highlights the importance of nonwoven membranes developed from degradable P(3HB/4HB) polymers in reducing inflammation, enhancing angiogenic properties of skin and facilitating better wound healing process.

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Section: Discussionmentioning

confidence: 99%

Experimental wound dressings of degradable PHA for skin defect repair

Shishatskaya

Nikolaeva

Vinogradova

et al. 2016

J Mater Sci: Mater Med

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“…A technology for preparing a 3D porous matrix ElastoPHB ® -3D on the basis of poly(hydroxybutyrate-co-hydroxyvalerate) co-polymer Bioplastotan ® , a component of film carrier ElastoPHB ® , was developed in Biomaterial Study Center, Institute of Transplantology and Artificial Organs [3,4]. The possibility of using ElastoPHB ® -3D matrixes for cell culturing with their subsequent transplantation was demonstrated [2].…”

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confidence: 99%

Micro-and nanostructural characteristics of 3D porous carriers ElastoPHB®-3D

et al. 2008

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Microstructure of the surface and micro- and nanostructure of the internal surface of 3D porous carrier ElastoPHB-3D were studied by methods of electron microscopy and atomic force microscopy. Biological properties of ElastoPHB-3D samples were evaluated using culture of L929 mouse fibroblasts. High porosity and pore size of biodegradable matrixes ElastoPHB-3D and their good biofunctional properties as the substrate for cell culturing allow us to recommend ElastoPH-3D as a promising carrier for cell transplantation and creation of artificial organs.

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ElastoPHB® Membrane Systems with Immobilized Bone Marrow Stromal Cells Optimize Conditions for Regeneration of Damaged Tissue

Cited by 2 publications

References 17 publications

Experimental wound dressings of degradable PHA for skin defect repair

Experimental wound dressings of degradable PHA for skin defect repair

Micro-and nanostructural characteristics of 3D porous carriers ElastoPHB®-3D

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