Elastomers in vascular tissue engineering

Hiob, Matti A.; Crouch, Gareth; Weiss, Anthony S.

doi:10.1016/j.copbio.2016.04.008

Cited by 28 publications

(21 citation statements)

References 65 publications

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“…Numerous tissues have been targeted in tissue engineering approaches including cartilage [ 1 ], skin, bone [ 2 ], teeth [ 3 ], blood vessels [ 4 ], and intestine [ 5 ]. Mesenchymal stromal cells (MSCs) are commonly employed in tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Transient cellular adhesion on poly(ethylene-glycol)-dimethacrylate hydrogels facilitates a novel stem cell bandage approach

et al. 2018

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We discovered a transient adhesion property in poly(ethylene glycol) dimethacrylate (PEG-DMA) hydrogels and employed it to develop a novel “stem cell bandage” model of cellular delivery. First, we cultured human mesenchymal stromal cells (MSCs) on the surface of PEG-DMA hydrogels with high amounts of arginine-glycine-aspartic acid (RGD) adhesive peptides (RGD++) or without RGD (RGD-). On day 1, MSCs underwent an initial adhesion to RGD- hydrogels that was not significantly different over 13 days (n = 6). In addition, cells appeared to be well spread by day 3. Significantly fewer cells were present on RGD- hydrogels on day 15 compared to day 9, suggesting that RGD- hydrogels allow for an initial cellular adhesion that is stable for multiple days, but transient over longer periods with a decrease by day 15. This initial adhesion is especially surprising considering that PEG-DMA does not contain any biological adhesion motifs and is almost chemically identical to poly(ethylene glycol) diacrylate (PEG-DA), which has been shown to be non-adhesive without RGD. We hypothesized that MSCs could be cultured on RGD- PEG-DMA hydrogels and then applied to a wound site to deliver cells in a novel approach that we refer to as a “stem cell bandage”. RGD- donor hydrogels were successfully able to deliver MSCs to PEG-DMA acceptor hydrogels with high RGD content (RGD++) or low amounts of RGD (RGD+). Our novel “bandage” approach promoted cell delivery to these model surfaces while preventing cells from diffusing away. This stem cell delivery strategy may provide advantages over more common stem cell delivery approaches such as direct injections or encapsulation and thus may be valuable as an alternative tissue engineering approach.

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Section: Introductionmentioning

confidence: 99%

Transient cellular adhesion on poly(ethylene-glycol)-dimethacrylate hydrogels facilitates a novel stem cell bandage approach

et al. 2018

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“…For decades, the production of synthetic vascular prostheses have involved mainly (1) polyethylene terephthalate (PET-commonly known as Terylene in the United Kingdom, Lavsan in Russia, or Dacron in the United States), and (2) polytetrafluoroethylene (PTFE-commonly known as Teflon or Gore-Tex). These are currently clinically available for vascular prostheses and both polymers are highly crystalline, which prevents plastic deformation under prolonged cyclical strain that would render the material unsuitable for vascular graft construction, 249 and hydrophobic, which decreases the internal reaction of water (hydrolysis) with internal esters of the polymer, hence prolonging degradation rate. 250 The hydrophobicity of these materials also prevents cell adhesion to the surface, where hydrophilicity resulting in swelling and strong interactions between the graft and blood is known to be thrombogenic.…”

Section: Engineering Vascular Graftsmentioning

confidence: 99%

Applied Bioengineering in Tissue Reconstruction, Replacement, and Regeneration

Colazo

Evans

Farinas

et al. 2019

Tissue Engineering Part B: Reviews

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To this day, tissue reconstruction and replacement to address extensive tissue defects due to trauma, tumor resection, genetic and/or chronic diseases, or excessive debridement present a major clinical challenge. Traditional reconstructive techniques most commonly utilize autologous tissue. Undoubtedly, autologous composite tissue transfers or ''flaps,'' skin grafts, as well as the harvesting of bone and/or cartilage have substantially improved the health, functional, and aesthetic outcomes of millions of patients. Unfortunately, these procedures are not without their drawbacks. These include increased operative time, complexity, cost, limited availability of qualitative autologous tissue, wound healing complications, tissue flap failure, and substantial donor-site morbidity. Recently, collaborative research teams-consisting of surgeons, scientists, and engineers-have made substantial progress in their attempts to solve these problems. This article provides historical perspective, covers the major limitations of current standards of care, and reviews recent advances and future prospects in applied bioengineering in the context of tissue reconstruction, replacement, and regeneration.

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“…However, although synthetic and even some naturally-derived polymers do not exhibit intrinsic cell supporting capabilities, they can be readily modified through the incorporation of bioactive peptides such as RGD, CAG, REDV, and YIGSR [59,60]. The different types of naturally-derived and synthetic-based biomaterials used in the development of tissue engineered myocardium [61][62][63], cardiac valves [64][65][66][67], and blood vessels [68][69][70][71][72] have been extensively reviewed in the literature and will not be discussed here.…”

Section: Biomimetic Design Of Biomaterials For Cardiovascular Tementioning

confidence: 99%

Biomimetic cardiovascular platforms for in vitro disease modeling and therapeutic validation

et al. 2019

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Bioengineered tissues have become increasingly more sophisticated owing to recent advancements in the fields of biomaterials, microfabrication, microfluidics, genetic engineering, and stem cell and developmental biology. In the coming years, the ability to engineer artificial constructs that accurately mimic the compositional, architectural, and functional properties of human tissues, will profoundly impact the therapeutic and diagnostic aspects of the healthcare industry. In this regard, bioengineered cardiac tissues are of particular importance due to the extremely limited ability of the myocardium to self-regenerate, as well as the remarkably high mortality associated with cardiovascular diseases worldwide. As novel microphysiological systems make the transition from bench to bedside, their implementation in high throughput drug screening, personalized diagnostics, disease modeling, and targeted therapy validation will bring forth a paradigm shift in the clinical management of cardiovascular diseases. Here, we will review the current state of the art in experimental in vitro platforms for next generation diagnostics and therapy validation. We will describe recent advancements in the development of smart biomaterials, biofabrication techniques, and stem cell engineering, aimed at recapitulating cardiovascular function at the tissue- and organ levels. In addition, integrative and multidisciplinary approaches to engineer biomimetic cardiovascular constructs with unprecedented human and clinical relevance will be discussed. We will comment on the implementation of these platforms in high throughput drug screening, in vitro disease modeling and therapy validation. Lastly, future perspectives will be provided on how these biomimetic platforms will aid in the transition towards patient centered diagnostics, and the development of personalized targeted therapeutics.

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Elastomers in vascular tissue engineering

Cited by 28 publications

References 65 publications

Transient cellular adhesion on poly(ethylene-glycol)-dimethacrylate hydrogels facilitates a novel stem cell bandage approach

Transient cellular adhesion on poly(ethylene-glycol)-dimethacrylate hydrogels facilitates a novel stem cell bandage approach

Applied Bioengineering in Tissue Reconstruction, Replacement, and Regeneration

Biomimetic cardiovascular platforms for in vitro disease modeling and therapeutic validation

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