Elastokine-mediated up-regulation of MT1-MMP is triggered by nitric oxide in endothelial cells

Interaction between the Elastin Peptide VGVAPG and Human Elastin Binding Protein

“…The importance of the QDEA string in VGVAPG interaction has already been suggested (5,27). Our work supports this view but also points at the possible importance of the SYNS string of V32.…”

“…Previous works (5,6,27) had emphasized that the VVGSP-SAQDEASPL sequence of EBP was important for elastin peptide binding. In particular, the hydrophilic QDEA string (residues 97-100) was identified as a potential interaction site (28).…”

Bioactive extracellular matrix fragments in lung health and disease

“…In 1984, Senior and colleagues examined the elastin repeating hexamer VGVAPG and found the peptide to be chemotactic to both monocytes and fibroblasts (59). Further studies demonstrated that this peptide upregulates various MMPs in endothelial cells, fibroblasts, and tumor cells (60)(61)(62)(63). The conformation of VGVAPG is critical to its biologic function (64), and the S-Gal receptor (also known as elastin-binding protein [EBP]) is thought to be the primary elastin receptor for this fragment (65) (Figure 1).…”

Elastin biology and tissue engineering with adult cells

“…The largest, EBP, is a 67-kDa cell-surface-associated protein that is a splice variant of β -galactosidase (69) . The other two subunits are membrane-bound, neuraminidase (Neu-1) and cathepsin A. Elastin-derived peptides bind the elastin receptor complex and induce up-regulation of genes, including pro-MMP1, in a Neu-1-dependent manner, providing evidence for a feedback loop in elastin maintenance (69,70) .…”