Elastohydrodynamic Lift at a Soft Wall

Davies, Heather; Débarre, Delphine; Amri, Nouha El; Verdier, Claude; Richter, Ralf P.; Bureau, Lionel

doi:10.1103/physrevlett.120.198001

Cited by 42 publications

(43 citation statements)

References 52 publications

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“…The second one is hydrodynamical: at low-Reynolds number the deformability of RBCs allows a symmetry breaking that leads to transverse migration from the wall to the center of the channel [10,11]. This symmetry breaking can also be obtained through the elastic deformation of the glycocalyx brush covering the endothelium [12]. Finally, RBC aggregation can have an influence on the CFL [5].…”

Section: Introductionmentioning

confidence: 99%

Migration velocity of red blood cells in microchannels

Losserand

Coupier

Podgorski

2019

Microvascular Research

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The lateral migration of red blood cells (RBCs) in confined channel flows is an important ingredient of microcirculatory hydrodynamics and is involved in the development of a cell free layer near vessel walls and influences the distribution of RBCs in networks. It is also relevant to a number of lab-on-chip applications. This migration is a consequence of their deformability and is due to the combined effects of hydrodynamic wall repulsion and the curvature of the fluid velocity profile. We performed microfluidic experiments with dilute suspensions of RBCs in which the trajectories and migration away from the channel wall are analyzed to extract the mean behavior, from which we propose a generic scaling law for the transverse migration velocity valid in a whole range of parameters relevant to microcirculatory and practical situations. Experiments with RBCs of different mechanical properties (separated by density gradient sedimentation or fixed with glutaraldehyde) show the influence of this parameter which can induce significant dispersion of the trajectories.

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Section: Introductionmentioning

confidence: 99%

Migration velocity of red blood cells in microchannels

Losserand

Coupier

Podgorski

2019

Microvascular Research

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“…One is based on the film indentation at rest by the gravitational force of beads of varying size. The other is based on a phenomenon called elastohydrodynamic lift -the lift of non-interacting beads on soft films under flow -which we recently reported [39].…”

Section: Estimation Of Ha Brush Stiffnessmentioning

confidence: 99%

“…Beads were injected onto HA films and left to settle for 5 min, and videos were then recorded in RICM under fixed flow rates. The flow rate, Q, was converted to shear stress, , by applying the following equation [39]:…”

Section: Flow Assay With Uncoated and Cd44+ Beadsmentioning

confidence: 99%

An integrated assay to probe endothelial glycocalyx-blood cell interactions under flow in mechanically and biochemically well-defined environments

et al. 2019

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Cell-cell and cell-glycocalyx interactions under flow are important for the behaviour of circulating cells in blood and lymphatic vessels. However, such interactions are not well understood due in part to a lack of tools to study them in defined environments. Here, we develop a versatile in vitro platform for the study of cell-glycocalyx interactions in well-defined physical and chemical settings under flow. Our approach is demonstrated with the interaction between hyaluronan (HA, a key component of the endothelial glycocalyx) and its cell receptor CD44. We generate HA brushes in situ within a microfluidic device, and demonstrate the tuning of their physical (thickness and softness) and chemical (density of CD44 binding sites) properties using characterisation with reflection interference contrast microscopy (RICM) and application of polymer theory. We highlight the interactions of HA brushes with CD44-displaying beads and cells under flow. Observations of CD44+ beads on a HA brush with RICM enabled the 3-dimensional trajectories to be generated, and revealed interactions in the form of stop and go phases with reduced rolling velocity and reduced distance between the bead and the HA brush, compared to uncoated beads. Combined RICM and bright-field microscopy of CD44+ AKR1 T-lymphocytes revealed complementary information about the dynamics of cell rolling and cell morphology, and highlighted the formation of tethers and slings, as they interacted with a HA brush under flow. This platform can readily incorporate more complex models of the glycocalyx, and should permit the study of how mechanical and biochemical factors are orchestrated to enable highly selective blood cell-vessel wall interactions under flow.

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“…For symmetrical objects, the results show that elastic deformations lead to a non-symmetric pressure field and to the emergence of a friction-reducing lift force. Of particular importance in nature are cases of freely moving particles close to soft surfaces as seen in flows of cells in vessels [25] or microfluidic devices [26,27], the mobility of suspended or falling objects near elastic membranes [28][29][30][31], the behavior of vesicles near walls [32] or the collisions between suspended particles [33]. It is only very recently that a theoretical work [34] addressed freely moving objects and showed how a free falling cylinder can sediment, slide and spin along a soft incline.…”

Section: Introductionmentioning

confidence: 99%

Rotation of a submerged finite cylinder moving down a soft incline

et al. 2020

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A submerged finite cylinder moving under its own weight along a soft incline lifts off and slides at a steady velocity while also spinning. Here, we experimentally quantify the steady spinning of the cylinder and show theoretically that it is due to a combination of an elastohydrodynamic torque generated by flow in the variable gap, and the viscous friction on the edges of the finitelength cylinder. The relative influence of the latter depends on the aspect ratio of the cylinder as well as the deformability of the substrate, which we express in term of a single scaled compliance parameter. By varying this compliance parameter, we show that our experimental results are consistent with a transition from an edge-effect dominated regime for short cylinders to a gapdominated elastohydrodynamic regime when the cylinder is very long.

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Elastohydrodynamic Lift at a Soft Wall

Cited by 42 publications

References 52 publications

Migration velocity of red blood cells in microchannels

Migration velocity of red blood cells in microchannels

An integrated assay to probe endothelial glycocalyx-blood cell interactions under flow in mechanically and biochemically well-defined environments

Rotation of a submerged finite cylinder moving down a soft incline

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