Elastin molecular aging promotes MDA‐MB‐231 breast cancer cell invasiveness

Abstract

Elastin is a long‐lived extracellular matrix protein responsible for the structural integrity and function of tissues. Breast cancer elastosis is a complex phenomenon resulting in both the deposition of elastotic masses and the local production of elastin fragments. In invasive human breast cancers, an increase in elastosis is correlated with severity of the disease and age of the patient. Elastin‐derived peptides ( EDP s) are a hallmark of aging and are matrikines – matrix fragments having the ability to regulate cell physiology. They are known to promote processes linked to tumor progression, but their effects on breast cancer cells remain unexplored. Our data show that EDP s enhance the invasiveness of MDA ‐ MB ‐231 breast cancer cells through the engagement of matrix metalloproteases 14 and 2. We therefore suggest that elastosis and/or an aged stroma could promote breast cancer cell invasiveness.

Elastin is a key factor of tumor development in colorectal cancer

“…ELN is a key component of the ECM family and plays critical roles in elasticity properties in soft tissues and may contribute to tumor formation. Previous studies have shown that ELN promotes human breast cancer cell invasiveness [7], indicating a potential role of ELN in cancer cell migration. However, the level of ELN in CRC has not been measured and the role of this molecule in CRC is not fully understood.…”

“…Human recombinant ELN (10 μg/ml, E6902, Sigma-Aldrich, St. Louis, USA) dissolved in sterile PBS was used to coat cell culture plates overnight at room temperature (RT). Control plates were coated with PBS only [7]. Human colon cancer epithelial cells (Caco-2, HTB-37, ATCC, Manassas, VA, USA) were seeded into each well of 24-or 96-well plate and cultured in Eagle's Minimum Essential Medium (EMEM) containing 2.5 mM L-glutamine, 10 mM HEPES and 10% FBS at 37°C with 5% CO 2 .…”

“…Collagen and elastin (ELN) are fibrous proteins that provide structural integrity and function of tissues. Collagen, a main component of ECM protein provides resistance and support to tissues, while ELN provides the characteristic elasticity properties in many soft tissues [7]. Abnormal level of ELN have been observed in many fibrotic diseases, including kidney [8], lung [9] and liver fibrosis [10].…”

“…The level of ELN in different cancers has been identified, and an accumulation of ELN fibre is associated with the development of hepatocellular carcinoma [12]. Increased degradation of ELN is found in human breast cancer due to increased activity of MMPs [7]. However, the level of ELN in CRC has not been measured and the role of this molecule in CRC is not well understood.…”

Transmembrane Peptides as Inhibitors of Protein-Protein Interactions: An Efficient Strategy to Target Cancer Cells?

“…Neu-1 is a member of the sialidase family composed also of Neu-2, Neu-3, and Neu-4 (40). These exoglycosidases, widely distributed amongst species (41), remove terminal sialic acid residues from glycoproteins, glycolipids, and oligosaccharides.…”