Elastic scattering spectroscopy for detection of cancer risk in Barrett’s esophagus: experimental and clinical validation of error removal by orthogonal subtraction for increasing accuracy

Zhu, Ying; Fearn, Tom; Mackenzie, Gary D.; Clark, Benjamin R.; Dunn, Jason M.; Bigio, Irving J.; Bown, Stephen G.; Lovat, Laurence

doi:10.1117/1.3194291

Cited by 32 publications

(22 citation statements)

References 28 publications

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“…EROS was reported to reduce complexity and increase accuracy of qualitative classification on colon lesions 35 and Barrett's esophagus. 36 Our results showed that short-term light probe pressure has negligible effects under clinical measurements conditions, thus we do not think it necessary to add an additional step in our postprocessing procedure. Furthermore, our current diagnostic model only used two parameters, thus reducing model complexity is not necessary.…”

Section: Alternatives To Alleviate Probe Pressure Effectsmentioning

confidence: 86%

Probe pressure effects on human skin diffuse reflectance and fluorescence spectroscopy measurements

et al. 2011

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Abstract. Diffuse reflectance and fluorescence spectroscopy are popular research techniques for noninvasive disease diagnostics. Most systems include an optical fiber probe that transmits and collects optical spectra in contact with the suspected lesion. The purpose of this study is to investigate probe pressure effects on human skin spectroscopic measurements. We conduct an in-vivo experiment on human skin tissue to study the short-term (<2 s) and long-term (>30 s) effects of probe pressure on diffuse reflectance and fluorescence measurements. Short-term light probe pressure (P0 < 9 mN/mm 2 ) effects are within 0 ± 10% on all physiological properties extracted from diffuse reflectance and fluorescence measurements, and less than 0 ± 5% for diagnostically significant physiological properties. Absorption decreases with site-specific variations due to blood being compressed out of the sampled volume. Reduced scattering coefficient variation is site specific. Intrinsic fluorescence shows a large standard error, although no specific pressure-related trend is observed. Differences in tissue structure and morphology contribute to site-specific probe pressure effects. Therefore, the effects of pressure can be minimized when the pressure is small and applied for a short amount of time; however, long-term and large pressures induce significant distortions in measured spectra. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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Section: Alternatives To Alleviate Probe Pressure Effectsmentioning

confidence: 86%

Probe pressure effects on human skin diffuse reflectance and fluorescence spectroscopy measurements

et al. 2011

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“…Elastic scattering spectroscopy (ESS) and related reflectance spectroscopies have shown promise in vivo in the gastrointestinal tract for detecting neoplasia in the colon, 18–22 dysplasia in the esophagus, 21,23–27 and colitis and dysplasia in patients with IBD. 18,19 ESS has also been used to distinguish pathologies in other epithelially lined hollow organs, such as the urinary bladder, 28 and in cystic and solid tissues, including breast and associated lymph nodes, 29,30 pancreas, 31 and thyroid.…”

mentioning

confidence: 99%

Elastic Scattering Spectroscopy as an Optical Marker of Inflammatory Bowel Disease Activity and Subtypes

Rodriguez‐Diaz

Atkinson

Jepeal

et al. 2014

Inflammatory Bowel Diseases

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Background In 10% to 15% of individuals, inflammatory bowel disease (IBD) is difficult to classify as ulcerative colitis (UC) or Crohn’s disease (CD). Previous work has demonstrated that probe-based elastic scattering spectroscopy (ESS) can produce spectra, informed by parameters like tissue ultrastructure and hemoglobin content, capable of differentiating pathologies. This study investigates whether ESS is an in vivo optical biomarker for the presence, activity, and type of IBD in the colon. Methods Pilot study, a retrospective data analysis. ESS spectra of endoscopically normal and inflamed colon were obtained from 48 patients with IBD and 46 non-IBD controls. Measurements from patients with IBD were categorized as CD or UC based on clinical diagnosis. Spectra were analyzed using high-dimensional methods. Leave-one-patient-out cross-validation was used to obtain diagnostic performance estimates. Results Patients with IBD were distinguishable from non-IBD controls with a sensitivity of 0.93 and specificity of 0.91 based on readings from endoscopically normal mucosa, and 0.94 and 0.93 from inflamed mucosa. In patients with IBD, histologically normal and inflamed colon were distinguishable with per-class accuracies of 0.83 and 0.89, respectively; histologically normal from inactive inflammation with accuracies of 0.73 and 0.89, respectively; and inactive from active colitis with accuracies of 0.87 and 0.84, respectively. The diagnosis of CD versus UC was made with per-class accuracies of 0.92 and 0.87 in normal and 0.87 and 0.85 in inflamed mucosa, respectively. Conclusions ESS, a simple, low-cost clinically friendly optical biopsy modality, has the potential to enhance the endoscopic assessment of IBD and its activity in real time and may help to distinguish CD from UC.

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“…The diameter of non-dysplastic prostate cell nuclei is typically 4 -6 μm, whereas dysplastic prostate nuclei can be as large as 10 μm [51]. These warning signs of the size distribution and chromatin content of epithelial-cell nuclei can be measured by light-scattering spectroscopy as an indicator of pre-invasive neoplasia [52,53], causing the blurring of optical imaging [54,55]. There is a growing interest in extracting microscopic properties of cancerous and normal cells by means of their measured optical parameters due to the differences of microstructure between these two types of tissues [56,57].…”

Section: Elastic Scattering Spectroscopy (Ess)mentioning

confidence: 99%

Optical biopsy - a new armamentarium to detect disease using light

Alfano

2015

Optical Biopsy XIII: Toward Real-Time Spectroscopic Imaging and Diagnosis

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Optical spectroscopy has been considered a promising method for cancer detection for past thirty years because of its advantages over the conventional diagnostic methods of no tissue removal, minimal invasiveness, rapid diagnoses, less time consumption and reproducibility since the first use in 1984. It offers a new armamentarium. Human tissue is mainly composed of extracellular matrix of collagen fiber, proteins, fat, water, and epithelial cells with key molecules in different structures. Tissues contain a number of key fingerprint native endogenous fluorophore molecules, such as tryptophan, collagen, elastin, reduced nicotinamide adenine dinucleotide (NADH), flavin adenine dinucleotide (FAD) and porphyrins. It is well known that abnormalities in metabolic activity precede the onset of a lot of main diseases: carcinoma, diabetes mellitus, atherosclerosis, Alzheimer, and Parkinson's disease, etc. Optical spectroscopy may help in detecting various disorders. Conceivably the biochemical or morphologic changes that cause the spectra variations would appear earlier than the histological aberration. Therefore, "optical biopsy" holds a great promise as clinical tool for diagnosing early stage of carcinomas and other deceases by combining with available photonic technology (e.g. optical fibers, photon detectors, spectrographs spectroscopic ratiometer, fiber-optic endomicroscope and nasopharyngoscope) for in vivo use. This paper focuses on various methods available to detect spectroscopic changes in tissues, for example to distinguish cancerous prostate tissues and/or cells from normal prostate tissues and/or cells. The methods to be described are fluorescence, stokes shift, scattering, Raman, and time-resolved spectroscopy will be reviewed. The underlying physical and biological basis for these optical approaches will be discussed with examples. The idea is to present some of the salient works to show the usefulness and methods of Optical Biopsy for cancer detection and show new directions.

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Elastic scattering spectroscopy for detection of cancer risk in Barrett’s esophagus: experimental and clinical validation of error removal by orthogonal subtraction for increasing accuracy

Cited by 32 publications

References 28 publications

Probe pressure effects on human skin diffuse reflectance and fluorescence spectroscopy measurements

Probe pressure effects on human skin diffuse reflectance and fluorescence spectroscopy measurements

Elastic Scattering Spectroscopy as an Optical Marker of Inflammatory Bowel Disease Activity and Subtypes

Optical biopsy - a new armamentarium to detect disease using light

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