Elaphuri Davidiani Cornu Improves Depressive-Like Behavior in Mice and Increases Neurotrophic Factor Expression in Mouse Primary Astrocytes via cAMP and ERK-Dependent Pathways

Zhu, Yue; Liu, Mengqiu; Qu, Suchen; Cao, Cheng; Wei, Chongqi; Meng, Xue-Er; Lou, Qianyin; Qian, Dawei; Duan, Jin‐Ao; Ding, Yulong; Han, Zhengxiang; Zhao, Ming

doi:10.3389/fphar.2020.593993

Cited by 5 publications

(4 citation statements)

References 24 publications

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“…Primary astrocyte cultures were prepared from the hippocampal tissue of postnatal BALB/c mice on day 1 as previously described [ 34 ]. Cell culture reagents for astrocytes were purchased from Thermo Fisher Scientific (Waltham, MA).…”

Section: Methodsmentioning

confidence: 99%

Scorpion venom peptide HsTx2 suppressed PTZ-induced seizures in mice via the circ_0001293/miR-8114/TGF-β2 axis

Meng

Yin

et al. 2022

J Neuroinflammation

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Background Due to the complexity of the mechanisms involved in epileptogenesis, the available antiseizure drugs (ASDs) do not meet clinical needs; hence, both the discovery of new ASDs and the elucidation of novel molecular mechanisms are very important. Methods BALB/c mice were utilized to establish an epilepsy model induced by pentylenetetrazol (PTZ) administration. The peptide HsTx2 was administered for treatment. Primary astrocyte culture, immunofluorescence staining, RNA sequencing, identification and quantification of mouse circRNAs, cell transfection, bioinformatics and luciferase reporter analyses, enzyme-linked immunosorbent assay, RNA extraction and reverse transcription–quantitative PCR, Western blot and cell viability assays were used to explore the potential mechanism of HsTx2 via the circ_0001293/miR-8114/TGF-β2 axis. Results The scorpion venom peptide HsTx2 showed an anti-epilepsy effect, reduced the inflammatory response, and improved the circular RNA circ_0001293 expression decrease caused by PTZ in the mouse brain. Mechanistically, in astrocytes, circ_0001293 acted as a sponge of endogenous microRNA-8114 (miR-8114), which targets transforming growth factor-beta 2 (TGF-β2). The knockdown of circ_0001293, overexpression of miR-8114, and downregulation of TGF-β2 all reversed the anti-inflammatory effects and the influence of HsTx2 on the MAPK and NF-κB signaling pathways in astrocytes. Moreover, both circ_0001293 knockdown and miR-8114 overexpression reversed the beneficial effects of HsTx2 on inflammation, epilepsy progression, and the MAPK and NF-κB signaling pathways in vivo. Conclusions HsTx2 suppressed PTZ-induced epilepsy by ameliorating inflammation in astrocytes via the circ_0001293/miR-8114/TGF-β2 axis. Our results emphasized that the use of exogenous peptide molecular probes as a novel type of ASD, as well as to explore the novel endogenous noncoding RNA-mediated mechanisms of epilepsy, might be a promising research area. Graphical Abstract

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Section: Methodsmentioning

confidence: 99%

Scorpion venom peptide HsTx2 suppressed PTZ-induced seizures in mice via the circ_0001293/miR-8114/TGF-β2 axis

Meng

Yin

et al. 2022

J Neuroinflammation

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“…Because of the rapid advancement of science and technology, the research on TCMs continues to deepen. Previous preclinical and clinical findings have revealed that free TCMs can play an essential role in treating the pathophysiology of many diseases, including cancer (Wang R. et al, 2020), diabetes , Alzheimer's disease , depression (Zhu et al, 2020), and COVID-19 (Pan et al, 2020;Tian J. et al, 2020). In addition, some researchers have begun to focus their study on how to treat CNS injuries more effectively by using exosomes as drug delivery vehicles, these carrying TCMs to specific areas and exerting therapeutic effects.…”

Section: Exosomes As Drug Delivery Vehicles For Traditional Chinese Medicines (Tcms)mentioning

confidence: 99%

Emerging Exosomes and Exosomal MiRNAs in Spinal Cord Injury

Feng

Zhang

Zhu

et al. 2021

Front. Cell Dev. Biol.

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Acute spinal cord injury (SCI) is a serious traumatic event to the spinal cord with considerable morbidity and mortality. This injury leads to short- and long-term variations in the spinal cord, and can have a serious effect on the patient’s sensory, motor, or autonomic functions. Due to the complicated pathological process of SCI, there is currently no successful clinical treatment strategy. Exosomes, extracellular vesicles (EVs) with a double-layer membrane structure of 30–150 nm diameter, have recently been considered as critical mediators for communication between cells and tissues by transferring proteins, lipids, and nucleic acids. Further studies verified that exosomes participate in the pathophysiological process of several diseases, including cancer, neurodegenerative diseases, and cardiovascular diseases, and could have a significant impact in their treatment. As natural carriers of biologically active cargos, exosomes have emerged as pathological mediators of SCI. In this review article, we critically discuss the functions of exosomes as intracellular mediators and potential treatments in SCI and provide an outlook on future research.

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“…As an important traditional Chinese medicine (TCM), EDC was first documented in Shennong Ben Cao Jing (approximately 200 B.C.-200 A.D.) and has been in TCM clinic use for thousands of years [6]. EDC was reported to have several biological activities, such as antiosteoporosis [7], antiaging [8], immuno-enhancing activity [9], and improving depressive-like behavior [6,10]. As the population of Père David's deer grows, the amount of shed EDC increases year by year [11], and it is expected to recover its medicinal value as soon as possible.…”

Section: Introductionmentioning

confidence: 99%

Discovery of Elaphuri Davidiani Cornu–specific peptide biomarkers by peptidomics analysis–based method

et al. 2023

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Elaphuri Davidiani Cornu (EDC) is the antler of the male Père David's deer, which has been reported to have multiple biological activities, and its use as a traditional Chinese medicine (TCM) in China has been known for thousands of years. However, EDC is difficult to distinguish from other related speciesderived antlers in powder or extract form in TCM clinic use, such as Cervus elaphus Cornu (CEC) and Cervus nippon Cornu (CNC), both derived from Cervidae and easily confused with EDC. In this study, a strategy using peptidomics combined with mathematics set analysis was used to identify EDC-specific peptide biomarkers, and four specific peptide biomarkers (Pep-E1-E4) were identified and validated. Pep-E1, Pep-E3, and Pep-E4 could be exclusively detected in EDC samples, with relative peak areas of 0.298 ± 0.060, 0.039 ± 0.015, and 0.037 ± 0.008, whereas Pep-E2 showed relative peak area of 0.516 ± 0.101 in EDC, 0.132 ± 0.026 in CEC, and 0.136 ± 0.047 in CNC samples, respectively. These four peptides are applicable to distinguish EDC from CEC and CNC, which is of great significance for the quality control of EDC.

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Elaphuri Davidiani Cornu Improves Depressive-Like Behavior in Mice and Increases Neurotrophic Factor Expression in Mouse Primary Astrocytes via cAMP and ERK-Dependent Pathways

Cited by 5 publications

References 24 publications

Scorpion venom peptide HsTx2 suppressed PTZ-induced seizures in mice via the circ_0001293/miR-8114/TGF-β2 axis

Scorpion venom peptide HsTx2 suppressed PTZ-induced seizures in mice via the circ_0001293/miR-8114/TGF-β2 axis

Emerging Exosomes and Exosomal MiRNAs in Spinal Cord Injury

Discovery of Elaphuri Davidiani Cornu–specific peptide biomarkers by peptidomics analysis–based method

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