Elaboration of toxic oxygen by-products by neutrophils in a model of immune complex disease.

Johnston, Richard B.; Lehmeyer, Joyce E.

doi:10.1172/jci108359

Cited by 237 publications

(73 citation statements)

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“…Recently, superoxide radicals have been implicated as playing an important role in neutrophi1 mediated cytotoxicity (9) and in causing endothelial damage by complement-stimulated granulocytes (10). In addition, superoxide is generated by neutrophils adherent to aggregated IgG-coated filters, a model of immune complex disease (7).…”

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confidence: 99%

Chemotactic factor‐induced generation of superoxide radicals by human neutrophils

Simchowitz

Mehta

Spilberg

1979

Arthritis & Rheumatism

140

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confidence: 99%

Chemotactic factor‐induced generation of superoxide radicals by human neutrophils

Simchowitz

Mehta

Spilberg

1979

Arthritis & Rheumatism

140

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“…Dimethylsulfoxide alone had no detectable effect on monocytes or neutrophils. Human IgG was aggregated by heat and fixed to micropore filters (10). The IgG is bound tightly to the filters in this system (10).…”

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confidence: 99%

“…was quantitated by its ability to reduce cytochrome c (1, 10). 2.5 × 10 e phagocytes or lymphocytes were incubated with 0.08 mM ferricytochrome c and with either opsonized zymosan or 5 ~g PMA at 37°C for 10 rain (2), or with IgG-coated filters for 5-120 min (10). Supernatant fluids were assayed spectrophotometrically (2, 10), and results were converted to nanomoles of cytochrome c reduced using hE550 nrn ~--" 2.1 × 104 M -I cm -I. Chemiluminescence was measured in a scintillation spectrometer (2,10).…”

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confidence: 99%

“…Supernatant fluids were assayed spectrophotometrically (2, 10), and results were converted to nanomoles of cytochrome c reduced using hE550 nrn ~--" 2.1 × 104 M -I cm -I. Chemiluminescence was measured in a scintillation spectrometer (2,10). When the stimulatory agent was opsenized zymosan or PMA, 107 phagocytes or lymphocytes were present in a reaction vol of 4 ml (2); 5 × 10 e cells in duplicate reaction vol of 1.5 ml were used with IgG-coated filters (10).…”

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confidence: 99%

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Generation of superoxide anion and chemiluminescence by human monocytes during phagocytosis and on contact with surface-bound immunoglobulin G.

Johnston¹,

Lehmeyer²,

Guthrie³

1976

The Journal of Experimental Medicine

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220

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Polymorphonuclear neutrophils and monocytes emigrate from the bloodstream to areas of inflammation and play an essential role there in a variety of physiological and pathological events, including defense against infection. Although the biochemical basis for these cells' microbicidal activity is not completely understood, studies primarily with neutrophils indicate that the killing of most organisms depends upon phagocytosis-associated oxidative metabolism. During phagocytosis neutrophils remove oxygen from the surrounding medium and convert it, probably first, to superoxide anion (O2") (1, 2) and then to hydrogen peroxide (H202) (3). H202 and O~. may interact to form the potent oxidant, hydroxyl radical (-OH) (2). Oxidation of glucose through the hexose monophosphate (HMP) shunt is stimulated, perhaps as a result of increased H202 generation (4). The spontaneous dismutation of O~. is believed to result in singlet oxygen formation (reviewed in 2), and the occurrence of this reaction in neutrophils appears to be the most likely explanation for the luminescence that occurs with phagocytosis (2, 5).Like neutrophils, monocytes undergo increased HMP shunt activation (6-8), oxygen consumption (7, 8), and H202 generation (7) during ingestion. Macrophages from mice and guinea pigs generate O~. (9). We report here that human monocytes elaborate O~. and generate chemiluminescence during phagocytosis, on stimulation by a surface-active chemical agent, and on contact with fLxed aggregated IgG. Materials and MethodsPreparation of Cells. Human neutrophils were separated from other leukocytes in 96-99% purity by centrifugation of defibrinated or heparinized venous blood through a Ficoll-Hypaque mixture (2) at 310 g for 35 min at room temperature. Erythrocytes were removed by dextran sedimentation and hypotonic lysis (2), and neutrophils were washed and suspended in KrebsRinger phosphate buffer, pH 7.35, containing 0.2% glucose and 0.2% bovine serum albumin. Monocyte-lymphocyte layers from the same preparations were diluted with 4 vol of buffer and centrifuged at 370 g, 4°C for 10 min. Erythrocytes were removed by hypotenic lysis, and the * Supported by U. S.

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“…ROS generation assays. Superoxide anion (02-) formation was determined according to the method of Johnston and Lehmeyer [7]. PMNs (106) were preincubated for 10 min with 0.2 ml opsonized zymosan at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Role of Reactive Oxygen Specie in Arthus Reaction

Yoshioka

Imamura

Niwa

1988

J. Clin. Biochem. Nutr.

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SummaryThe effect of serum obtained from Arthus reaction-elicited rabbits on the generation of reactive oxygen species (ROS) by normal rabbits' polymorphonuclear leukocytes (PMNs) was examined.. The oxidative metabolism of infiltrating PMNs in the skin during the Arthus reaction was observed by the nitroblue tetrazolium dye reduction test (NBT test). The significantly increased H2O2 generation by normal PMNs was observed when PMNs were incubated with serum obtained 24 and 48 h after the elicitation. Also the increased O2 -generation was noted after incubation with serum taken at 48 h after the elicitation. Many NBT-positive PMNs were observed in skin specimens obtained 24 and 48 h after the elicitation. These results may indicate the importance of ROS generated by infiltrating PMNs in immune complex vasculitis because the Arthus reaction is a model of immune complex vasculitis.Key Words: Arthus reaction, immune complex vasculitis, reactive oxygen species, skin NBT test The experimental Arthus reaction, which is a model of immune complex vasculitis, is similar to necrotizing vasculitis both macroscopically and histologically. In its pathogenesis, immune complexes (ICs) deposited on the wall of blood vessels activate the complement system with resultant production of chemotactic factors such as Coa. Then, attracted polymorphonuclear leukocytes (PMNs) produce various inflammatory mediators, and induce tissue damage in the wall of the blood vessels. These reactions are observed macroscopically as redness, induration, and bleeding, and histologically as dense infiltration of PMNs, fibrinoid degeneration of blood vessels, and nuclear dusts. In rabbits, the Arthus reaction was suppressed both macroscopically and histologically when colchicine was injected intraperitoneally before elicitation [1], indicating that PMNs play an im-*To whom correspondence should be addressed .

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Elaboration of toxic oxygen by-products by neutrophils in a model of immune complex disease.

Cited by 237 publications

References 31 publications

Chemotactic factor‐induced generation of superoxide radicals by human neutrophils

Chemotactic factor‐induced generation of superoxide radicals by human neutrophils

Generation of superoxide anion and chemiluminescence by human monocytes during phagocytosis and on contact with surface-bound immunoglobulin G.

Role of Reactive Oxygen Specie in Arthus Reaction

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