Elaboration of novel extraction procedure to reveal bioactive component profile of anthocyanin-rich plants

Abstract

Kiss A., Rapi S., Korozs M., Forgo P. (2014): Elaboration of novel extraction procedure to reveal bioactive component profile of anthocyanin-rich plants. Czech J. Food Sci., 32: 384-390.The content of anthocyanin derivatives, antioxidant activity, and phenolic content were determined in black elderberry (sambucus nigra L.), sweet cherry (prunus avium L.), blackberry (Rubus fruticosus L.), black currant (Ribes nigrum L.), and blackthorn (prunus spinosa L.). The extraction efficiency was examined of several solvents including hot water, 2% phosphoric acid, ethanol and acetone. A new sequential (cascade) extraction procedure was developed in order to improve the efficiency of the conventional methods. This novel extraction protocol consists of 3 different steps with the prevalence of low pH extraction conditions. When comparing the effectiveness of the conventional and presently improved procedures, it was stated that significantly increased anthocyanin yields had been observed. The highest anthocyanin content, determined with HPLC method, was found in the case of sweet cherry (222.7 mg/kg) on using the three step extraction procedure. The highest antioxidant activity determined with DPPH method was also assigned to the sweet cherry sample (5272 mg/kg). The highest phenolic content was found in blackberry (434 mg/kg).

Effect of chokeberry juice consumption on antioxidant capacity, lipids profile and endothelial function in healthy people: a pilot study

“…The DPPH method is frequently used to analyse the antioxidant capacity of fruit and vegetable juices (Vinson et al 1998(Vinson et al , 2001Seeram et al 2008;Kiss et al 2014). Recently, efforts have been made to use it for the determination of the antioxidant activity of blood plasma of humans or animals, e.g.…”

The Distribution of Minerals in Crucial Plant Parts of Various Elderberry (Sambucus spp.) Interspecific Hybrids

“…Among plant species, especially among common fruit and vegetable plants, the black elderberry ( Sambucus nigra ) is considered as an important source of nutrition, characterized by high color capacity, antioxidant activity [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ], and relatively large amounts of minerals [ 21 , 22 ]. According to the taxonomy of Bolli [ 23 ], S. nigra represents one of the nine species of the genus Sambucus which belongs to the family Adoxaceae [ 24 ].…”