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Expression of Ca-Binding Protein Recoverin in Normal, Surviving, and Regenerating Retina of Pleurodeles Waltl Adult Triton

“…waltl retina. On the whole, the results of recoverin location in normal triton retina coincided with our previous fi ndings [1]. Recoverinpositive staining in tangential sections was seen only in the external retina, while the internal part of the retina, growth area (pars ciliaris -ora serrata), and pigmented epithelium of the retina were recoverinnegative.…”

“…Other photoreceptor proteins are also highly conservative, for example, rhodopsin, arrestin, transducin α-subunit, and cGMP phosphodiesterase [25]. According to our data (the present and previous [1] fi ndings), the pattern of recoverin distribution in normal triton retinal cones and rods somewhat differs from that in the majority of mammals. In mammalian photoreceptors, recoverin is present mainly in the external, while in triton it is detected mainly in the internal segments of photoreceptors.…”

“…Bovine antibodies to recoverin are capable of cross-reacting with triton recoverin [1] and with recoverin ortholog S-modulin present in frog retina [17,18]. Since amphibians are an animal class far from mammals, we can speak about highly conservative nature of recoverin.…”

“…The data on the localization of recoverin and its homologues in Urodela retina are scanty: the fi ndings of our previous study on Pleurodeles waltl [1] and detection of recoverin in tiger salamander photoreceptors [23,33]. Special interest to studies of recoverin expression in tritons is explained primarily by unique capacity of these animals to regeneration of the retina after its excision or after crossing of the optic nerve [15,22] and to regeneration after long-lasting detachment of the retina from the pigmented epithelium [2].…”

“…waltl retina protein extract [1]. Using polyclonal (monospecifi c) antibodies to recoverin, we carried out an immunohistochemical study of the location of this protein in Pl.…”