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Cotsaftis, Olivier; Sallaud, Christophe; Breitler, Jean Christophe; Meynard, Donaldo; Greco, Rafaella; Pereira, Andy; Guiderdoni, Emmanuel

doi:10.1023/a:1020380305904

Cited by 86 publications

(8 citation statements)

References 37 publications

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“…Once the GOI jumps away from the SMG in the genome and becomes genetically unlinked, segregation can result in GOI-only plants. Cotsaftis et al [61] have used this approach to obtain insect-resistance rice without an SMG. It is worth noting that the additional advantage of using a GOI re-position-mediated-by-transposon strategy is that a large population of transgenic lines with the target gene (GOI) can be generated for evaluation for position effects, which is very useful for species which are not amenable for efficient genetic transformation, such as wheat.…”

Section: Co-transformation (Transfer Goi and Smg Separately) And Segrmentioning

confidence: 99%

Less is more: strategies to remove marker genes from transgenic plants

2013

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Selectable marker genes (SMGs) and selection agents are useful tools in the production of transgenic plants by selecting transformed cells from a matrix consisting of mostly untransformed cells. Most SMGs express protein products that confer antibiotic- or herbicide resistance traits, and typically reside in the end product of genetically-modified (GM) plants. The presence of these genes in GM plants, and subsequently in food, feed and the environment, are of concern and subject to special government regulation in many countries. The presence of SMGs in GM plants might also, in some cases, result in a metabolic burden for the host plants. Their use also prevents the re-use of the same SMG when a second transformation scheme is needed to be performed on the transgenic host. In recent years, several strategies have been developed to remove SMGs from GM products while retaining the transgenes of interest. This review describes the existing strategies for SMG removal, including the implementation of site specific recombination systems, TALENs and ZFNs. This review discusses the advantages and disadvantages of existing SMG-removal strategies and explores possible future research directions for SMG removal including emerging technologies for increased precision for genome modification.

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Section: Co-transformation (Transfer Goi and Smg Separately) And Segrmentioning

confidence: 99%

Less is more: strategies to remove marker genes from transgenic plants

2013

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“…The system based on the Streptomyces phage phiC31 integrase (Thorpe and Smith 1998) has been successfully applied for the elimination of selectable markers from transgenic plants and is particularly attractive, given its irreversibility. A second strategy relies on the activity of transposon systems; here, the selectable marker gene is flanked by sequences recognized by a transposase, so that when the cassette is introduced, the selectable marker is mobilized to a new location in the genome, thereby becoming separated from the GOI (Belzile et al 1989; Cotsaftis et al 2002; Gorbunova and Levy 2000). A third strategy involves the introduction of the selectable marker and the GOI on separate T-DNA sequences, and relies on their integration sites being different.…”

Section: Introductionmentioning

confidence: 99%

The elimination of a selectable marker gene in the doubled haploid progeny of co-transformed barley plants

et al. 2012

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Following the production of transgenic plants, the selectable marker gene(s) used in the process are redundant, and their retention may be undesirable. They can be removed by exploiting segregation among the progeny of co-transformants carrying both the selectable marker gene and the effector transgene. Here we show that the doubled haploid technology widely used in conventional barley breeding programmes represents a useful means of fixing a transgene, while simultaneously removing the unwanted selectable marker gene. Primary barley co-transformants involving hpt::gfp (the selectable marker) and gus (a model transgene of interest) were produced via Agrobacterium-mediated gene transfer to immature embryos using two respective T-DNAs. These plants were then subjected to embryogenic pollen culture to separate independently integrated transgenes in doubled haploid progeny. A comparison between 14 combinations, involving two Agrobacterium strains carrying various plasmids, revealed that the highest rate of independent co-transformation was achieved when a single Agrobacterium clone carried two binary vectors. Using this principle along with Agrobacterium strain LBA4404, selectable marker-free, gus homozygous lines were eventually obtained from 1.5 per 100 immature embryos inoculated. Compared to the segregation of uncoupled T-DNAs in conventionally produced progeny, the incorporation of haploid technology improves the time and resource efficiency of producing true-breeding, selectable marker-free transgenic barley.Electronic supplementary materialThe online version of this article (doi:10.1007/s11103-012-9988-9) contains supplementary material, which is available to authorized users.

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“…Specific techniques and modified plant transformation approaches have been developed to remove such marker genes from transgenic crops. Techniques such as transposon-mediated elimination of marker gene [97], intrachromosomal homologous recombination [98], site-specific recombination Cre/LoxP [99], and FLP/FRT [100] systems have been developed. Modified transformation techniques such as co-transformation of marker genes and target genes (gene of interest) and excision of the marker gene in subsequent generations by genetic segregation are some methods for marker-free transgenic rice development [95,101].…”

Section: Development Of Golden Ricementioning

confidence: 99%

Rice Biofortification: High Iron, Zinc, and Vitamin-A to Fight against “Hidden Hunger”

Majumder

Datta

2019

Agronomy

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One out of three humans suffer from micronutrient deficiencies called “hidden hunger”. Underprivileged people, including preschool children and women, suffer most from deficiency diseases and other health-related issues. Rice (Oryza sativa), a staple food, is their source of nutrients, contributing up to 70% of daily calories for more than half of the world’s population. Solving “hidden hunger” through rice biofortification would be a sustainable approach for those people who mainly consume rice and have limited access to diversified food. White milled rice grains lose essential nutrients through polishing. Therefore, seed-specific higher accumulation of essential nutrients is a necessity. Through the method of biofortification (via genetic engineering/molecular breeding), significant increases in iron and zinc with other essential minerals and provitamin-A (β-carotene) was achieved in rice grain. Many indica and japonica rice cultivars have been biofortified worldwide, being popularly known as ‘high iron rice’, ‘low phytate rice’, ‘high zinc rice’, and ‘high carotenoid rice’ (golden rice) varieties. Market availability of such varieties could reduce “hidden hunger”, and a large population of the world could be cured from iron deficiency anemia (IDA), zinc deficiency, and vitamin-A deficiency (VAD). In this review, different approaches of rice biofortification with their outcomes have been elaborated and discussed. Future strategies of nutrition improvement using genome editing (CRISPR/Cas9) and the need of policy support have been highlighted.

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Cited by 86 publications

References 37 publications

Less is more: strategies to remove marker genes from transgenic plants

Less is more: strategies to remove marker genes from transgenic plants

The elimination of a selectable marker gene in the doubled haploid progeny of co-transformed barley plants

Rice Biofortification: High Iron, Zinc, and Vitamin-A to Fight against “Hidden Hunger”

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