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Abstract

The labile SAUR transcripts from higher plants contain a conserved DST sequence in their 3'-untranslated regions. Two copies of a DST sequence from soybean are sufficient to destabilize reporter transcripts in cultured tobacco cells whereas variants bearing mutations in the conserved ATAGAT or GTA regions are inactive. To investigate the potential for conserved recognition components in mammalian and plant cells, we examined the function of this instability determinant in mouse NIH3T3 fibroblasts and tobacco BY2 cells. In fibroblasts, a tetrameric DST element from soybean accelerated deadenylation and decay of a reporter transcript. However, a version mutated in the ATAGAT region was equally effective in this regard, and a tetrameric DST element from Arabidopsis was inactive. In contrast, the soybean DST element was more active as an mRNA instability element than the mutant version and the Arabidopsis element, when tested as tetramers in tobacco cells. Hence, the plant DST element is not recognized in animal cells with the same sequence requirements as in plant cells. Therefore, its mode of recognition appears to be plant-specific.

A novel adenylate isopentenyltransferase 5 regulates shoot branching via the ATTTA motif in Camellia sinensis

“…AU-rich elements (AREs) are sequence elements of 50-150 nt that are rich in adenosine and uridine bases and are frequently found in the 3′ UTRs of labile mRNAs [ 15 , 16 ]. In higher eukaryotes, the AUUUA motif, which are among the best-characterized mRNA-destabilizing determinants, and other AREs could decrease gene expression and inhibit mRNA translation (i.e., protein synthesis), at least partially by enhancing the rate of its deadenylation and subsequent mRNA degradation [ 16 – 22 ].…”

“…In the presence of three AUUUA sequences in the 3′ UTR, transcripts of tobacco ( N. tabacum ) par gene have shorter lifes [ 20 ]. Mutations in the AUUUA motifs increase mRNA stability and enhance mRNA accumulation [ 17 , 24 ], whereas insertion of AUUUA motifs into a stable mRNA destabilizes the mRNA levels [ 15 , 20 ]. ATTTA and other AREs-specific stability and deadenylation of mRNA appear to be regulated by development and stress [ 17 , 20 – 22 ].…”

Identification of unstable transcripts in Arabidopsis by cDNA microarray analysis: Rapid decay is associated with a group of touch- and specific clock-controlled genes

“…This instability determinant is found in the 3Ј UTR of the small auxin up RNA (SAUR) genes. DST elements have a complex structure (15) and the recognition requirements appear to be unique to plants (16). Other sequences that cause instability have also been described (17)(18)(19).…”

In silicio expression analysis of PKS genes isolated from Cannabis sativa L.

“…Several studies suggest that the untranslated regions (UTRs) are important for the control of gene expression in plants at the post-transcriptional level. Stability (Feldbrugge et al , 2001; Schwartz et al , 2006; Vaucheret, 2006), transport (Li and Hunt, 1997; Siomi, 2000), and translation (Klaff et al , 1996; Guo et al , 2000; Geslain and Ribas de Pouplana, 2004) of RNA depend on the 5'-UTR, the 3'-UTR, or both. We believe that variation in the size of UTRs from these PKS cDNAs could be the result of alternative transcription initiation and polyadenylation sites from post-transcriptional processing of PKS pre-mRNAs (Dean et al , 1986; Joshi, 1987; Rothnie, 1996).…”