El proteoma del tejido adiposo subcutáneo muestra heterogeneidad anatómica

Martos‐Moreno, Gabriel Ángel; Sackmann-Sala, Lucila; Berryman, Darlene E.; Blome, D.W.; Argente, Jesús; Kopchick, John J.

doi:10.1016/j.anpedi.2012.10.010

Cited by 9 publications

(6 citation statements)

References 39 publications

(49 reference statements)

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“…All of the 2DE, image processing and analysis, mass spectrometry (MS) and tandem MS (MS-MS) analysis procedures (MALDI-TOF) have been detailed previously for human samples [9-12,15]. Identities obtained after MS and MS-MS analysis were verified using the MS and MS/MS data obtained to search online databases with the software Mascot (http://www.matrixscience.com) [9-12,15].…”

Section: Methodsmentioning

confidence: 99%

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Proteomic analysis allows for early detection of potential markers of metabolic impairment in very young obese children

Martos‐Moreno

Sackmann-Sala

Barrios

et al. 2014

Int J Pediatr Endocrinol

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BackgroundEarly diagnosis of initial metabolic derangements in young obese children could influence their management; however, this impairment is frequently not overt, but subtle and undetectable by routinely used clinical assays. Our aim was to evaluate the ability of serum proteomic analysis to detect these incipient metabolic alterations in comparison to standard clinical methods and to identify new candidate biomarkers.MethodsA cross-sectional study of fasting serum samples from twenty-two prepubertal, Caucasian obese (OB; 9.22 ± 1.93 years; 3.43 ± 1.08 BMI-SDS) and twenty-one lean controls (C; 8.50 ± 1.98 years; -0.48 ± 0.81 BMI-SDS) and a prospective study of fasting serum samples from twenty prepubertal, Caucasian obese children (11 insulin resistant [IR]) before (4.77 ± 1.30 BMI-SDS) and after weight reduction (2.57 ± 1.29 BMI-SDS) by conservative treatment in a reference hospital (Pros-OB) was performed. Proteomic analysis (two-dimension-eletrophoresis + mass spectrometry analysis) of serum and comparative evaluation of the sensitivity of routinely used assays in the clinics to detect the observed differences in protein expression level, as well as their relationship with anthropometric features, insulin resistance indexes, lipid profile and adipokine levels were carried out.ResultsStudy of the intensity data from proteomic analysis showed a decrease of several isoforms of apolipoprotein-A1, apo-J/clusterin, vitamin D binding protein, transthyretin in OBvs. C, with some changes in these proteins being enhanced by IR and partially reversed after weight loss. Expression of low molecular weight isoforms of haptoglobin was increased in OB, enhanced in IR and again decreased after weight loss, being positively correlated with serum interleukin-6 and NAMPT/visfatin levels. After statistical correction for multiple comparisons, significance remained for a single isoform of low MW haptoglobin (OB vs. C and IR vs. non-IR) and Apo A1 (IR vs. non-IR). Assays routinely used in the clinical setting (ELISA/kinetic nephelometry), only partially confirmed the changes observed by proteomic analysis (ApoA1 and haptoglobin).ConclusionProteomic analysis can allow for the identification of potential new candidate biomarkers as a complement to routinely used assays to detect initial changes in serum markers of inflammation and lipid metabolism impairment in young obese children.

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Section: Methodsmentioning

confidence: 99%

“…Identities obtained after MS and MS-MS analysis were verified using the MS and MS/MS data obtained to search online databases with the software Mascot (http://www.matrixscience.com) [9-12,15]. …”

Section: Methodsmentioning

confidence: 99%

Proteomic analysis allows for early detection of potential markers of metabolic impairment in very young obese children

Martos‐Moreno

Sackmann-Sala

Barrios

et al. 2014

Int J Pediatr Endocrinol

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“…Two-dimensional electrophoresis coupled to MALDI TOF was used to identify proteins that are differentially expressed in visceral white adipose tissue between obese/overweight and control groups and to study the role of the identified proteins in adipocyte differentiation [ 26 ]. Obesity is a well-known causative factor for cardiovascular disease; 2-DE coupled to MALDI TOF was used by Moreno et al to study the anatomical heterogeneity in the proteome of human subcutaneous adipose tissue to identify a close relationship between obesity and cardiovascular disease [ 27 ]. Total body adiposity is known to correlate well with the amount of adipose tissue around the myocardium and the coronary arteries, the so-called epicardial adipose tissue.…”

Section: Separation Techniquesmentioning

confidence: 99%

Obesity Proteomics: An Update on the Strategies and Tools Employed in the Study of Human Obesity

Masood

Alfadda

2018

High-Throughput

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Proteomics has become one of the most important disciplines for characterizing cellular protein composition, building functional linkages between protein molecules, and providing insight into the mechanisms of biological processes in a high-throughput manner. Mass spectrometry-based proteomic advances have made it possible to study human diseases, including obesity, through the identification and biochemical characterization of alterations in proteins that are associated with it and its comorbidities. A sizeable number of proteomic studies have used the combination of large-scale separation techniques, such as high-resolution two-dimensional gel electrophoresis or liquid chromatography in combination with mass spectrometry, for high-throughput protein identification. These studies have applied proteomics to comprehensive biochemical profiling and comparison studies while using different tissues and biological fluids from patients to demonstrate the physiological or pathological adaptations within their proteomes. Further investigations into these proteome-wide alterations will enable us to not only understand the disease pathophysiology, but also to determine signature proteins that can serve as biomarkers for obesity and related diseases. This review examines the different proteomic techniques used to study human obesity and discusses its successful applications along with its technical limitations.

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“…Several studies devoted to understanding the physiological differences between visceral and SQ WAT have been performed, but few studies have addressed the molecular and functional differences between SQ WAT fat depots. To determine the proteomic differences in WAT depots, our laboratory conducted two studies, one in humans and the other in mice, using various adipose depots (Table 2 ; Additional file 2 : Table S2) [ 104 , 105 ].…”

Section: Main Textmentioning

confidence: 99%

“…To determine the proteomic differences in WAT depots, our laboratory conducted two studies, one in humans and the other in mice, using various adipose depots (Table 2 ; Additional file 2 : Table S2) [ 104 , 105 ].…”

Section: Main Textmentioning

confidence: 99%

The impact of growth hormone on proteomic profiles: a review of mouse and adult human studies

2017

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Growth hormone (GH) is a protein that is known to stimulate postnatal growth, counter regulate insulin’s action and induce expression of insulin-like growth factor-1. GH exerts anabolic or catabolic effects depending upon on the targeted tissue. For instance, GH increases skeletal muscle and decreases adipose tissue mass. Our laboratory has spent the past two decades studying these effects, including the effects of GH excess and depletion, on the proteome of several mouse and human tissues. This review first discusses proteomic techniques that are commonly used for these types of studies. We then examine the proteomic differences found in mice with excess circulating GH (bGH mice) or mice with disruption of the GH receptor gene (GHR−/−). We also describe the effects of increased and decreased GH action on the proteome of adult patients with either acromegaly, GH deficiency or patients after short-term GH treatment. Finally, we explain how these proteomic studies resulted in the discovery of potential biomarkers for GH action, particularly those related with the effects of GH on aging, glucose metabolism and body composition.Electronic supplementary materialThe online version of this article (doi:10.1186/s12014-017-9160-2) contains supplementary material, which is available to authorized users.

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El proteoma del tejido adiposo subcutáneo muestra heterogeneidad anatómica

Cited by 9 publications

References 39 publications

Proteomic analysis allows for early detection of potential markers of metabolic impairment in very young obese children

Proteomic analysis allows for early detection of potential markers of metabolic impairment in very young obese children

Obesity Proteomics: An Update on the Strategies and Tools Employed in the Study of Human Obesity

The impact of growth hormone on proteomic profiles: a review of mouse and adult human studies

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