El efecto de una solución superoxidada sobre el uroepitelio de la vejiga de ratas

Abstract

Aim: The present study aimed to identify the changes on the bladder mucosa in rats after intravesical infusion of Super-oxidized solution (SOS) (Mycrodacyn60® Mexico).
 Design: Eighteen Wistar Albino rats weighing 250-300 g were randomly categorized into three groups. Rats in Group 1 were infused with a single dose of 1.5 ml of 0.9% saline solution (SS) whereas those in group 2 were administered a single dose of 1.5 ml pH neutral SOS. Control rats in Group 3 did not undergo any procedure.
 Results: The absence of bacteria was verified before and after the administration of the solution, performing urine cultures and amplifying the 16S rRNA fragment by PCR and histopathological examinations for each bladder. The absence of bacterial DNA in the uroepithelium was demonstrated, histopathological examination revealed, that irrigation with SOS, in histological observation, no structural alterations were identified in the urothelial mucosa.
 Limitations: It was not possible to analyze the effect of SOS absorption indamage to the uroepithelium secondary to traumatic infection or injury. The effect of irrigation at different times and volumes with SOS was also not analyzed.
 Originality: It is known that SOS is useful as disinfectant in skin and serosa bacterial infections, lacking toxicity and it is effective against Escherichia coli. The use of superoxide solutions in cavities such as the bladder, has not been investigated yet.
 Conclusion: The pH-neutral SOS infused transurethral in the bladder of rats has no adverse effects on the uroepithelium, which was supported by histopathological evidence.

The rationale for bladder washouts in children with neurogenic bladder

“…Multiple studies have shown no adverse effects on the uroepithelium of rodents. 31,32 ECASs are already used in cardiothoracic and dental surgery 28 while HOCl is widely used as a disinfectant, sanitizer, and in wound care. 33 Importantly, HOCl is effective against biofilms, while antibiotic therapy generally fails to affect biofilms.…”

“…Multiple studies have shown no adverse effects on the uroepithelium of rodents 31,32 . ECASs are already used in cardiothoracic and dental surgery 28 while HOCl is widely used as a disinfectant, sanitizer, and in wound care 33 .…”