El caracol tintóreo (Plicopurpura pansa) en los municipios de Jicalapa y Chiltiupán, La Libertad, El Salvador

Rivera, Ana María

doi:10.5377/ryr.v54i54.12085

Cited by 1 publication

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“…), [ 36–39 ] hNSCs require animal sourced substrates (Matrigel gel, Geltrex, and a feeder cell layer) (e.g., WiCell NSC‐H14, NSC‐H14; ATCC ACS‐5001, 5003–5006, etc.). [ 40–44 ] When hNSCs are cultured on nonanimal sourced substrates, they do not adhere to them and instead, anoikis or differentiation could be induced though the mechanism is not well understood. It has been a challenge in the field to find a cost‐effective, xeno‐free, and chemically defined growth substrate for hNSC maintenance for decades.…”

Section: Introductionmentioning

confidence: 99%

Effect of Degree of Deacetylation of Chitosan/Chitin on Human Neural Stem Cell Culture

Chang

Zhou

James

et al. 2022

Macromolecular Bioscience

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Stem cell therapy and research for neural diseases depends on reliable reproduction of neural stem cells. Chitosan-based materials have been proposed as a substrate for culturing human neural stem cells (hNSCs) in the pursuit of clinically compatible culture conditions that are chemically defined and compliant with good manufacturing practices. The physical and biochemical properties of chitosan and chitin are strongly regulated by the degree of deacetylation (DD). However, the effect of DD on hNSC behavior has not been systematically investigated. In this study, films with DD ranging from 93% to 14% are fabricated with chitosan and chitin. Under xeno-free conditions, hNSCs proliferate preferentially on films with a higher DD, exhibiting adherent morphology and retaining multipotency. Lowering the DD leads to formation of neural stem cell spheroids due to unsteady adhesion. The neural spheroids present NSC multipotency protein expression reduction and cytoplasmic translocation. This study provides an insight into the influence of the DD on hNSCs behavior and may serve as a guideline for hNSC research using chitosan-based biomaterials. It demonstrates the capability of controlling hNSC fate by simply tailoring the DD of chitosan.

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Section: Introductionmentioning

confidence: 99%