Ekstraksi dan Identifikasi Senyawa Flavonoid dari Limbah Kulit Bawang Merah sebagai Antioksidan Alami

Rahayu, Siti; Kurniasih, Nunung; Amalia, Vina

doi:10.15575/ak.v2i1.345

Cited by 51 publications

(61 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hal ini didukung dengan adanya penelitian sebelumnya yang menyatakan bahwa ekstrak atanol dari kulit bawang merah (Allium cepa) memiliki aktivitas terhadap Eschericia coli, Pseudomonas fluoroscens, Staphylococcus aureus dan Bacillus cereus serta jamur Aspergillus niger, Trichoderma viride, dan Penicillium cyclopium (Skerget et al, 2009;Misna & Khusnul, 2016). Metabolit sekunder yang terkandung pada bagian kulit dari bawang merah di antaranya yaitu alkaloid, flavonoid, terpenoid, saponin, polifenol, dan kuersetin yang memiliki aktivitas sebagai antimikroba (Soemari, 2016;Rahayu et al, 2015).…”

Section: Pendahuluanunclassified

Antimicrobial Activity of Ethanol Extract of Shallot (Allium cepa L.) Peels Using the Disc Diffusion Method

Octaviani¹,

Fadhli²,

Yuneistya³

2019

psr

View full text Add to dashboard Cite

ABSTRAKBawang merah (Allium cepa L.) secara umum digunakan sebagai bumbu masakan oleh masyarakat. Bagian dari bawang merah yang banyak dimanfaatkan adalah bagian umbinya saja, sedangkan bagian kulit terluar dari bawang merah tersebut dibuang karena hanya dianggap sebagai limbah. Berdasarkan hasil skrining fitokimia, ekstrak kulit bawang merah mengandung senyawa fenolik, flavonoid dan terpenoid yang dapat memberikan daya hambat terhadap pertumbuhan mikroorganisme. Tujuan dari penelitian ini adalah untuk melihat aktivitas antimikroba ekstrak etanol dari kulit bawang merah terhadap Staphylococcus epidermidis dan Staphylococcus aureus sebagai bakteri Gram positif, Salmonella thypi dan Eschericia coli sebagai bakteri Gram negatif serta aktivitas antijamur terhadap jamur Trichophyton mentagrophytes. Penelitian ini menggunakan metode difusi cakram dengan beberapa konsentrasi ekstrak etanol dari kulit bawang merah yaitu 50%, 25%, 12,5%, 6,25%, 3,125% dan 1,5625% b/v serta kontrol positif kloramfenikol untuk bakteri, kontrol positif nistatin untuk jamur dan DMSO sebagai kontrol negatif. Diameter hambat yang dihasilkan pada pengujian ekstrak etanol dari kulit bawang merah terhadap bakteri Staphylococcus epidermidis, Staphylococcus aureus, Salmonella thypi dan Eschericia coli dengan konsentrasi 50% berturut-turut adalah 11,75 mm; 16,03 mm; 9,42 mm dan 7,77 mm. Diameter hambat yang dihasilkan pada pengujian ekstrak etanol dari kulit bawang merah terhadap jamur Trichophyton mentagrophytes dengan konsentrasi 50% adalah 18,53 mm. Sebagai kesimpulan, ekstrak etanol kulit bawang merah memiliki aktivitas dalam menghambat pertumbuhan bakteri Staphylococcus aureus, Staphylococcus epidermidis, Salmonella thypi, Escherichia coli dan jamur Trichophyton mentagrophytes.ABSTRACT Shallots (Allium cepa L.) are generally used as cooking ingredients by the community. The part of the shallot widely used is only a part of the tuber, while the outer shell of the shallot is thrown away because it is only considered as wastes. Based on phytochemical screening results, extract of shallot peels contains phenolics, flavonoids, and terpenoids that can inhibit the growth of microorganisms. The purpose of this research is to know the antimicrobial activity of ethanol extract of shallot peels against Staphylococcus epidermidis and Staphylococcus aureus as Gram positive bacteria, Salmonella thypi and Eschericia coli as Gram negative bacteria and also antifungal activity against Trichophyton mentagrophytes. The study was performed using disc diffusion method with the variation of concentration of ethanol extract of the peels of 50%, 25%, 12.5%, 6.25%, 3.125% and 1.5625% w/v, respectively, the positive control of chloramphenicol for bacteria, the positive control of nystatin for fungi and the negative control of DMSO. The diameter of the inhibition zone formed on the activity assay of ethanol extract of the shallot peels against Staphylococcus epidermidis, Staphylococcus aureus, Salmonella thypi and Eschericia coli at the concentration of 50% was 11.75 mm, ...

show abstract

Section: Pendahuluanunclassified

Antimicrobial Activity of Ethanol Extract of Shallot (Allium cepa L.) Peels Using the Disc Diffusion Method

Octaviani¹,

Fadhli²,

Yuneistya³

2019

psr

View full text Add to dashboard Cite

show abstract

“…This study was aimed at detecting active antimicrobial substances in the ethanol extracts of M. calabura leaves using GC-MS. M. calabura leaf extracts were prepared by maceration, and the yield obtained was 24.7%. Maceration yields depend on the length of time of maceration, the presence or absence of stirring, and the number of re-macerations (Rahayu et al, 2015). In addition to these physical factors, they are also affected by biological and chemical determinants.…”

Section: Resultsmentioning

confidence: 99%

Active antimicrobial substances of strawberry tree leaf extracts (Muntingia calabura L.) against Methicillin-Resistant Staphylococcus aureus (MRSA) based on GC-MS analysis

2020

View full text Add to dashboard Cite

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the bacteria that triggers nosocomial diseases. Bacterial resistance requires continuous exploration of active antimicrobial substances from various sources, including medicinal plants. Leaves of cherry (Muntingia calabura L.) reportedly contain three classes of compounds, namely, tannins, flavonoids, and saponins. This research was designed to identify active antimicrobial substances in cherry leaves that could inhibit the growth of MRSA. It employed the Kirby-Bauer test to examine the antimicrobial activities of the leaf extracts of Muntingia calabura L. (EMC) against MRSA. Through GC-MS, active substances were detected from the presence of active spots on TLC plates, as determined by direct-contact bioautography. The TLC used silica gel F254 as the stationary phase and chloroform:ethyl acetate (9:1) as the mobile phase. The antimicrobial activity test results showed that the zone of inhibition of 10% w/v EMC was 10.91±0.75 mm in diameter. At 5% w/v and 2.5% w/v, EMC created zones of inhibition with diameters of 8.5±0.25 mm and 7.25±0.25 mm, respectively. Meanwhile, at 1.25% w/v, it showed no inhibitory activities. Based on the TLC-Bioautography profile, the active spot that produced zones of inhibition was located at Rf 0.04 mm. The GC-MS analysis of this spot detected the presence of two compounds: the first compound had a similarity index of 35% with 3,11,13triacetycynaratriol, and the second one had a similarity index of 80% with hexaborane-12. Cynaratriol is known to posses antimicrobial activity, whereas hexaborane is the opposite. In conclusion, the minimum inhibitory concentration of EMC for MRSA is 2.5% w/v. Also, the active compounds of EMC bear 35% similarities to 3,11,13-triacetycynaratriol.

show abstract

“…Bawang merah banyak digunakan dalam kehidupan sehari-hari sebagai bumbu masak, dan bahan obatobatan. Kandungan gizi bawang merah cukup tinggi dimana setiap 100 gram umbi bawang merah mengandung 88 g air, 9,2 g karbohidrat, 1,5 g protein, 0,3 g lemak, 0,03 mg kalori (Rahayu dan Berlian, 2004).…”

Section: Pendahuluanunclassified

Pengaruh Pemberian Pupuk Hayati dan Pupuk N, P, K Terhadap Pertumbuhan dan Produksi Tanaman Bawang Merah (Allium ascalonicum L.)

Maruf

Nelvia

Silvina

2019

View full text Add to dashboard Cite

The research aims to study the effect of the application ultra gen biofertilizer and N, P, K fertilizer on the growth and production of onion (Allium ascalonicum L.). The research was conducted at Agricultural Faculty Experimental Unit, University of Riau, Pekanbaru. The research in form factorial experimentally 5x3 arranged in a completely randomized design. The first factor was ultra gen biofertilizer consists of 5 levels (without, 50, 100, 150 and 200 l.ha-1). The second factor was a N, P, K fertilizer consists of 3 levels (without, 100 kg N + 45 kg P2O5 + 37,5 kg K2O and 200 kg N + 90 kg P2O5 + 75 kg K2O.ha-1), each combination was repeated 3 times. The parameters observed were number of bulb per clump, circumference of bulb, weight of bulb fresh per clump, weight of bulb fresh per plot and weight of bulb consumption per plot. The application of ultra gen biofertilizer doses of 100 l.ha-1and N, P, K fertilizer 100 kg N + 45 kg P2O5 + 37,5 kg K2O.ha-1 increased weight of bulb fresh per clump, weight of bulb fresh per plot and weight of bulb consumption per plot significantly compared to another combination, but not significantly on number of bulb per clump and circumference of bulb.

show abstract

Ekstraksi dan Identifikasi Senyawa Flavonoid dari Limbah Kulit Bawang Merah sebagai Antioksidan Alami

Cited by 51 publications

References 3 publications

Antimicrobial Activity of Ethanol Extract of Shallot (Allium cepa L.) Peels Using the Disc Diffusion Method

Antimicrobial Activity of Ethanol Extract of Shallot (Allium cepa L.) Peels Using the Disc Diffusion Method

Active antimicrobial substances of strawberry tree leaf extracts (Muntingia calabura L.) against Methicillin-Resistant Staphylococcus aureus (MRSA) based on GC-MS analysis

Pengaruh Pemberian Pupuk Hayati dan Pupuk N, P, K Terhadap Pertumbuhan dan Produksi Tanaman Bawang Merah (Allium ascalonicum L.)

Contact Info

Product

Resources

About