Ejaculate and type of freezing extender affect rates of fertilization of horse oocytes in vitro

Roasa, L.M.; Choi, Young Ho; Love, C.C.; Romo, S.; Varner, D.D.; Hinrichs, Katrin

doi:10.1016/j.theriogenology.2007.04.057

Cited by 22 publications

(10 citation statements)

References 35 publications

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“…Although some groups have reported modest rates of fertilization [1][2][3][4][5], these methods have not been repeatable. Failure of equine IVF is related to the failure of equine sperm to penetrate the zona pellucida in vitro: when the zona is breached by partial zona dissection or dissolution, it results in high fertilization rates and even polyspermy [6,7].…”

Section: Introductionmentioning

confidence: 99%

Hyperactivated Sperm Motility: Are Equine Sperm Different?

Hinrichs

Loux

2012

Journal of Equine Veterinary Science

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Section: Introductionmentioning

confidence: 99%

Hyperactivated Sperm Motility: Are Equine Sperm Different?

Hinrichs

Loux

2012

Journal of Equine Veterinary Science

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“…Unfortunately, neither this nor other equine IVF protocols have proven to be reproducible. Indeed, subsequent attempts have yielded extremely poor equine IVF results, with reported cleavage rates varying from 0 to 33% (Zhang et al 1990, Choi et al 1994, Li et al 1995, Dell'aquila et al 1996, 1997a,b, Alm et al 2001, Hinrichs et al 2002, Tremoleda et al 2003, Roasa et al 2007, Mugnier et al 2009a,b, Lange-Consiglio & Cremonesi 2012. A detailed overview of the published equine IVF studies was produced by Leemans et al (2016a).…”

Section: Equine Ivf: An Updatementioning

confidence: 99%

Update on mammalian sperm capacitation: how much does the horse differ from other species?

Leemans¹,

Stout²,

Schauwer³

et al. 2019

Reproduction

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In contrast to various other mammalian species, conventional in vitro fertilization (IVF) with horse gametes is not reliably successful. In particular, stallion spermatozoa fails to penetrate the zona pellucida, most likely due to incomplete activation of stallion spermatozoa (capacitation) under in vitro conditions. In other mammalian species, specific capacitation triggers have been described; unfortunately, none of these is able to induce full capacitation in stallion spermatozoa. Nevertheless, knowledge of capacitation pathways and their molecular triggers might improve our understanding of capacitation-related events observed in stallion sperm. When sperm cells are exposed to appropriate capacitation triggers, several molecular and biochemical changes should be induced in the sperm plasma membrane and cytoplasm. At the level of the sperm plasma membrane, (1) an increase in membrane fluidity, (2) cholesterol depletion and (3) lipid raft aggregation should occur consecutively; the cytoplasmic changes consist of protein tyrosine phosphorylation and elevated pH, cAMP and Ca 2+ concentrations. These capacitation-related events enable the switch from progressive to hyperactivated motility of the sperm cells, and the induction of the acrosome reaction. These final capacitation triggers are indispensable for sperm cells to migrate through the viscous oviductal environment, penetrate the cumulus cells and zona pellucida and, finally, fuse with the oolemma. This review will focus on molecular aspects of sperm capacitation and known triggers in various mammalian species. Similarities and differences with the horse will be highlighted to improve our understanding of equine sperm capacitation/fertilizing events.Reproduction (2019) 157 R181-R197

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“…In horses, in vitro fertilization problems are related to sperm entry into the oocyte, and, specifically, to the penetration of the zona pellucida (Alm et al, 2001;Roasa et al, 2007). To date there are only two foals that were obtained with the use of conventional fertilization (Palmer, 1991).…”

Section: In Vitro Fertilization Of Matured Oocytesmentioning

confidence: 99%

Obtaining farm animal embryos <i>in vitro</i>

Duszewska¹,

Rapala²,

Trzeciak³

et al. 2012

J. Anim. Feed Sci.

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This paper reviews the basic knowledge about obtaining farm animal embryos in vitro with special focus on differences among species and application of this procedure in the future. In vitro production of farm animal embryos consists of in vitro maturation (IVM) of oocytes, in vitro fertilization (IVF) of matured oocytes, and in vitro culture (IVC) of embryos. Oocytes can be collected from live animals (by laparotomy, laparoscopy, Ovum Pick Up) or from slaughtered ones (by puncture, sectioning). Usually immature oocytes are isolated, and during IVM they reach maturity. Matured oocytes are cultured with sperm (IVF), leading to the formation of zygotes. In the case of fertilization problems (horse, pig), intracytoplasmic sperm injection is used. The zygotes are usually cultured (IVC) to the morula and blastocyst stages. These embryos can be transferred to recipients or frozen/vitrified. Offspring have been obtained after transfer of cattle, sheep, goat, pig and horse embryos. This procedure can be used in animal breeding, biotechnology, medicine, and basic research.

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Ejaculate and type of freezing extender affect rates of fertilization of horse oocytes in vitro

Cited by 22 publications

References 35 publications

Hyperactivated Sperm Motility: Are Equine Sperm Different?

Hyperactivated Sperm Motility: Are Equine Sperm Different?

Update on mammalian sperm capacitation: how much does the horse differ from other species?

Obtaining farm animal embryos <i>in vitro</i>

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