Eisenia andrei Behavioral and Antioxidative Responses to Excess of Copper in the Soil

Clasen, Bárbara; Ferreira, Talita; Santana, Natielo Almeida; Portela, Valéria Ortaça; Loro, Vania Lúcia; Murussi, Camila; Leitemperger, Jossiele; Tiecher, Tadeu Luís; Domínguez, Jorge; Jacques, Rodrigo Josemar Seminoti; Storck, Tamiris Rosso; Antoniolli, Zaida Inês

doi:10.1007/s11270-021-05395-7

Cited by 4 publications

(3 citation statements)

References 52 publications

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“…In the present work, a cocoon production inversely proportional to the Cu soil concentration was observed, in agreement with other literature evidence (Clasen et al 2021;Duan et al 2016;Tatsi et al 2018). For example, Owojori et al (2010) found that a soil Cu concentration above 80 mg/ kg resulted in a significant decrease of cocoon production as compared to the control.…”

Section: Avoidance and Reproductive Outputssupporting

confidence: 92%

“…Earthworm trophic activity decreased following high Cu exposure, as reported by other authors (Tatsi et al 2018). Similar biomass trends with a weight gain up to a critical Cu concentration followed by a significant weight loss at higher concentrations was also shown in other experiments (De Bernardi et al 2022b;Clasen et al 2021;Owojori et al 2010); these occurrences are common in the presence of toxicants and are clearly associated to hormesis phenomena. In the earthworm reproduction test by Lukkari et al (2005) conducted on a high organic content substrate (~ 7%), the biomass of A. tuberculata was increased at intermediate Cu doses ranging from 53 to 79 mg/kg, while it decreased at the highest tested concentrations ranging from 119 to 268 mg/kg.…”

Section: Avoidance and Reproductive Outputssupporting

confidence: 85%

“…Earthworms are considered good indicators of the occurrence and toxicity of both inorganic and organic pollutants (Jager et al 2003;Vijver et al 2003); for this reason, Eisenia fetida (E. fetida) is employed in standard ecotoxicity tests (ISO-17512-1, 2008;OECD 2016OECD , 1984. Nevertheless, there are still some uncertainties regarding the effects that Cu excess can have on earthworms' reproduction (fertility parameters) and development (biomass growth) and their relation with other endpoints such as oxidative damages (Clasen et al 2021).…”

Section: Introductionmentioning

confidence: 99%

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Copper Toxicity on Eisenia Fetida in a Vineyard Soil: A Combined Study with Standard Tests, Genotoxicity Assessment, and Gut Metagenomic Analysis

Marini,

De Bernardi,

Tagliabue

et al. 2023

Preprint

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Section: Avoidance and Reproductive Outputssupporting

confidence: 92%

Section: Avoidance and Reproductive Outputssupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Copper Toxicity on Eisenia Fetida in a Vineyard Soil: A Combined Study with Standard Tests, Genotoxicity Assessment, and Gut Metagenomic Analysis

Marini,

De Bernardi,

Tagliabue

et al. 2023

Preprint

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Copper toxicity on Eisenia fetida in a vineyard soil: a combined study with standard tests, genotoxicity assessment and gut metagenomic analysis

Marini,

De Bernardi,

Tagliabue

et al. 2024

Environ Sci Pollut Res

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Copper (Cu) toxicity is a pressing concern for several soils, especially in organic viticulture. The objective of this work was to assess Cu toxicity on the non-target organism Eisenia fetida, employing both traditional and novel tools for early identification of Cu-induced damages. In addition to traditional tests like avoidance and reproductive toxicity experiments, other tests such as the single cell gel electrophoresis (SCGE) and gut microbiome analysis were evaluated to identify early and more sensitive pollution biomarkers. Four sub-lethal Cu concentrations were studied, and the results showed strong dose-dependent responses by the earthworm avoidance test and the exceeding of habitat threshold limit at the higher Cu doses. An inverse proportionality was observed between reproductive output and soil Cu concentration. Bioaccumulation was not detected in earthworms; soil concentrations of potentially bioavailable Cu were not affected by E. fetida presence or by time. On the contrary, the SCGE test revealed dose-dependent genotoxicity for the ‘tail length’ parameter already at the second day of Cu exposition. Gut microbiome analysis a modulation of microbial composition, with the most aboundant families being Pectobateriaceae, Comamonadaceae and Microscillaceae. Bacillaceae increased over time and showed adaptability to copper up to 165 mg/kg, while at the highest dose even the sensitive Acetobacteriaceae family was affected. The research provided new insights into the ecotoxicity of Cu sub-lethal doses highlighting both alterations at earthworms’ cellular level and changes in their gut microbiota.

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FRET-based innovative assays for precise detection of the residual heavy metals in food and agriculture-related matrices

Shen

Nie

Wei

et al. 2022

Coordination Chemistry Reviews

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Eisenia andrei Behavioral and Antioxidative Responses to Excess of Copper in the Soil

Cited by 4 publications

References 52 publications

Copper Toxicity on Eisenia Fetida in a Vineyard Soil: A Combined Study with Standard Tests, Genotoxicity Assessment, and Gut Metagenomic Analysis

Copper Toxicity on Eisenia Fetida in a Vineyard Soil: A Combined Study with Standard Tests, Genotoxicity Assessment, and Gut Metagenomic Analysis

Copper toxicity on Eisenia fetida in a vineyard soil: a combined study with standard tests, genotoxicity assessment and gut metagenomic analysis

FRET-based innovative assays for precise detection of the residual heavy metals in food and agriculture-related matrices

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