Einkorn (Triticum monococcum ssp. monococcum) in vitro propagation sterilization protocol

Örgeç, Mehmet; Karakaş, Fatma Pehlivan; Şahin, Günce; Ağıl, Ferdi; Zencirci, Nüsret

doi:10.21448/ijsm.399094

Cited by 8 publications

(4 citation statements)

References 12 publications

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“…De-hulled einkorn and bread wheat seeds were washed by five drops Tween 20 (Merck, Darmstadt, Germany supplemented) 100 mL distilled water for one minute, then, disinfected with 40% commercial bleach (4.6% NaClO; Domestos, Istanbul, Turkey) with a magnetic stirrer for 15 min in a laminar air flow. Seeds were, then, washed with sterile water to remove chemicals from seeds at the end (Örgeç et al 2018 ).…”

Section: Methodsmentioning

confidence: 99%

In vitro mature embryo culture protocol of einkorn (Triticum monococcum ssp. monococcum) and bread (Triticum aestivum L.) wheat under boron stress

Ağıl

Örgeç

Karakaş

et al. 2021

Plant Cell Tiss Organ Cult

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Mature embryos of einkorn ( Triticum monococcum ssp. monococcum ) and bread ( Triticum aestivum L.) wheat were used for callus induction on media containing four different doses (0, 1, 2 and 4 mg L −1 ) of 2,4-D and dicamba supplemented with five different boron concentrations (0, 6.2, 12.4, 24.8, and 37.2 mg L −1 ). The obtained callus was transferred to culture media with three (0, 0.5, and 2 mg L −1 ) different BAP doses with five boron concentrations for further regeneration. The maximum callus weight in einkorn wheat was in culture media with 1 mg L −1 dicamba and 6.2 mg L −1 (3.71 ± 0.13 g). Bread wheat had the maximum callus weight on culture media with 4 mg L −1 dicamba and 12.4 mg L −1 (3.46 ± 0.40 g). The highest plantlet numbers were in only 2 mg L −1 BAP (2.92 ± 0.88) for einkorn wheat and 0.5 mg L −1 BAP supplemented with 6.2 mg L −1 boron (3.71 ± 1.12) for bread wheat. This indirect regeneration protocol using mature embryos of einkorn and bread wheat under boron stresses expected to be useful for future wheat breeding studies.

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Section: Methodsmentioning

confidence: 99%

In vitro mature embryo culture protocol of einkorn (Triticum monococcum ssp. monococcum) and bread (Triticum aestivum L.) wheat under boron stress

Ağıl

Örgeç

Karakaş

et al. 2021

Plant Cell Tiss Organ Cult

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“…NaClO is also a highly effective sterilant against all kinds of bacteria, fungi, and viruses. Increasing the NaClO concentration and exposure time resulted in low survival percentages, and, on the other hand, contamination increased with lower concentrations of NaCIO [25][26][27]. NaClO is usually applied with other chemicals in sterilization.…”

Section: Sterilization Stagementioning

confidence: 99%

Optimization of Conditions for In Vitro Culture of Selected Arbutus unedo L. Genotypes

Durul

Memis

2022

Agronomy

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The strawberry tree is an evergreen shrub tree that grows on sea-facing slopes under a Mediterranean climate. It has a key role in the lives of the rural inhabitants of forested areas and is one of the most promising species for developing new approaches in cultivation for improved fruit. As a result of the growing interest of farmers, selection studies have focused on the selection of highly productive varieties of Arbutus unedo, whereby there is a requirement for the selected varieties to be propagated at a large scale. In this study, the in vitro propagation potential of the selected A. unedo genotypes, YV2 and YV4, was investigated. The best results in terms of decreasing browning with a high level of sterilization were achieved with 50% ethanol (1 min) and 20% NaCIO (12 min) for both shoot tip and nodium explants. In terms of growth performance, both genotypes displayed the highest proliferation rate in WPM medium-supplemented with BAP (2.0 mg/L) and with added IBA and GA3 (0.1 mg/L each), in which the shoot number and elongation were also the highest. YV2 microcuttings showed higher rooting rates when cultured in WPM medium, with an added 2.0 mg/L IBA, whereas the addition of 4.0 mg/L NAA was optimal for YV4. The resulting root quality was sufficient for surviving the acclimatization stage. The genotype effect was clear in all stages during the in vitro propagation for all growth parameters.

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“…In addition to the difficulties related to rootstocks rooting, a persistent presence of unidentified endogenous microorganisms is common in micropropagated plants, even with good practices in asepsis, a rigorous establishment, and several successive in vitro transfers [21,22]. This phenomenon was also observed in Prunus avium (plum) and Malus sp.…”

Section: Introductionmentioning

confidence: 99%

Endophytic Bacteria Can Replace the Need for Synthetic Auxin during In Vitro Rooting of Pyrus communis

et al. 2022

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This study aimed to investigate the presence of beneficial microorganisms in the micropropagation of Pyrus communis rootstocks through the isolation, identification, and selection of auxin-producing bacteria. The selected microorganisms were also inoculated in vitro to evaluate their effect on the plant rooting process and their behavior during the acclimatization phase. The results showed the presence of endophytic microorganisms in plant tissue cultures of the ‘OH×F87′ and ‘PDW’ selections. A low diversity was observed in the population of microorganisms isolated from the in vitro culture of the ‘OH×F87’and ‘PDW’ selections, with a predominance of the genera Acinetobacter, Bacillus, and Buttiauxella. The selection of promoting microorganisms was performed based on the auxin production test, in which 30.36% of the microorganisms tested positive. In the in vitro inoculation, it was possible to observe the promotion of growth and emission of roots in the pear rootstocks, from the inoculation with bacteria identified as capable of producing auxin. This process can be used to replace the inclusion of synthetic auxin in the productive chain of woody fruit trees propagated by vegetative means, such as pears. None of the bacterial isolates was notably more promising, but the general similarity of treatments containing the A. septicus and A. ursingii strains, with the synthetic auxin treatment, suggests the possibility of its use on a large scale allowing the adoption of the cheaper method. of rooting. This work opens the door for further research using new, more promising microbial isolates, and also for lower-cost microorganism cultivation techniques, such as low-cost media obtained from agro-industrial residues.

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Einkorn (Triticum monococcum ssp. monococcum) in vitro propagation sterilization protocol

Cited by 8 publications

References 12 publications

In vitro mature embryo culture protocol of einkorn (Triticum monococcum ssp. monococcum) and bread (Triticum aestivum L.) wheat under boron stress

In vitro mature embryo culture protocol of einkorn (Triticum monococcum ssp. monococcum) and bread (Triticum aestivum L.) wheat under boron stress

Optimization of Conditions for In Vitro Culture of Selected Arbutus unedo L. Genotypes

Endophytic Bacteria Can Replace the Need for Synthetic Auxin during In Vitro Rooting of Pyrus communis

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