Eine verbesserung der enzymatischen bestimmung von H2O2. anwendung auf die glucose-bestimmung

Pütter, J.; Strufe, R.

doi:10.1016/0009-8981(67)90341-5

Cited by 8 publications

(2 citation statements)

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“…The rate of production of the fluorescence of oxidized HVA is measured and related to peroxide concentration. Putter and S trufe added polyvinylpyrrolidine compounds for stabilization of color in the peroxidase-o-dianisidine procedure for peroxidase (249). Schulz, Passonneau, and Lowry (282) have described a fluorometric enzymic method for the measurement of inorganic phosphate based on the following enzyme sequence.…”

Section: Acid + Nadmentioning

confidence: 99%

Use of enzymes in analytical chemistry

Guilbault¹

1968

Anal. Chem.

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Both of these contain valuable procedures for enzyme analysis. Guilbault (97) has authored a chapter on fluorometric methods of analysis of enzymes, substrates, activators, and inhibitors in his book, "Fluorescence. Theory, Instrumentation and Practice." Passwater's book,(233) "Guide to the Umbelliferone (7 -hydroxy coumarin) and 4-methyl-umbelliferone are highly fluorescent compounds which have been modified to form nonfluorescent substrates for the enzymes glucuronidase, glucosidase, Ar-acetyl-/3-glucosaminidase, and ß-galactosidase. Robinson (259) used 4-methylumbelliferone^-Dglucoside as a substrate for ß-glucosidase. The substrate is split specifically by this enzyme, thus permitting the separate identification of ß-glucosidase and ß-glucuronidase in paper electrophoresis. Woolen and Turner (358, 359) assayed ß-glucuronidase in plasma using 4-methyl umbelliferylß-glucuronide as substrate, and Woolen and Walker (360), proposed 4-methyl umbelliferone-.Y-acetyl -ß-D-glucosaminide and 4-methyl umbelliferone-ß-D-galactoside as substrates for V-acetylß-D-glucosaminidase and ß-galactosidase. Likewise, Leaback (169) described conditions for the continuous fluorometric assay of Y-acetyl^-Dglucosaminidase using the same substrate, and Veritz, Gambell, and Brown (336) proposed l-naphthyl-2-acetamido-2-deox3T^-D-glucop3"ranoside as a substrate for this enzyme. The production of 1-naphthol was proportional to the concentration of enzyme.Greenberg (96) assayed ß-glucuroni-

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Section: Acid + Nadmentioning

confidence: 99%

Use of enzymes in analytical chemistry

Guilbault¹

1968

Anal. Chem.

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“…The following catechol compounds were purchased from the companies given in parentheses and used without further purification: norepinephrine hydrogentartrate and dopamine hydrochloride (Wako, Tokyo, Japan); isoproterenol hydrochloride, 4 . Dobutamine hydrochloride and carbidopa (both, catecholic drugs) were supplied by Shionogi (Osaka, Japan) and Sankyo (Tokyo, Japan), respectively.…”

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confidence: 99%

Assay for Peroxidase Using 1,2-DiarylethyIenediamines and Catechol Compounds as Fluorogenic Substrates

et al. 1991

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A novel method for the fluorometric assay of horseradish peroxidase and microperoxidase activities is described based on their catalytic reactions between 1,2-diarylethylenediamines and catechol compounds as fluorogenic substrates in the presence of hydrogen peroxide. meso-l,2-Diphenylethylenediamine and epinephrine as a couple of substrates are most recommendable in practical use. This method is highly sensitive: the detection limits (S/ N=2) are 10 µU tube-1 for horseradish peroxidase and 500 fmol tube-1 for microperoxidase.Keywords Horseradish peroxidase, microperoxidase, activity assay, fluorometry, 1,2-diarylethylenediamine, meso-1,2-diphenylethylenediamine, epinephrinePeroxidase has been used as a marker enzyme in immunoassay and DNA hybridization assay because of its high stability. Activity is commonly assayed by colorimetric or fluorometric methods: the former uses 4-aminoantipyrine-phenols, 2,2'-azino-di(3-ethylbenzothiazoline-6-sulfonic acid)2, benzidine3 or di-o-anisidine;4 the latter uses p-substituted phenolic compounds, such as homovanillic acids, tyramine6, p-cresol' and phydroxyphenylpropionic acids as substrates. Although the fluorometric methods are more sensitive than the spectrophotometric methods, the reaction products from the substrates fluoresce in too short wavelength region to observe with the naked eye as in DNA hybridization assay.In our previous papers, it was shown that 1,2-diarylethylenediamines (DAEs) react with catechol compounds in the presence of potassium hexacyanoferrate(III) to provide intense fluorescence (emission maxima, longer than 480 nm).9-12 We have found that this reaction can be mediated by peroxidase (HRP) and microperoxidase (MP) in the presence of hydrogen peroxide.This paper describes the enzymatic reactivities of various DAEs with catechol compounds and the characteristics of the yielding fluorescence, and optimal conditions of the assay for HRP and MP using meso-l,2-diphenylethylenediamine (DPE) and epinephrine as a selected pair of substrates. Experimental Reagents, solutions and apparatusThe following 29 DAEs were synthesized as described previously: s s DPE, 1,2-bis(2-hydroxyphenyl)ethylenediamine, 1,2-bis(2-, 3-and 4-methoxyphenyl)ethylenediamines, 1,2-bis(2-and 4-ethoxyphenyl)ethylenediamines, 1,2-bis(2-, 3-and 4-methylphenyl)ethylenediamines, 1,2-bis(4-ethylphenyl)ethylenediamine, 1,2-bis(3,4-dimethoxyphenyl)ethylenediamine, 1,2-bis(3,4-methylenedioxyphenyl)ethylenediamine, 1,2-bis(4-fluorophenyl)ethylenediamine, 1,2-bis(2-, 3-and 4-chlorophenyl)ethylenediamines, 1,2-bis(2,6-and 3,4-dichlorophenyl)ethylenediamines, 1,2-bis(4-cyanophenyl)ethylenediamine, 1,2-bis(4-dimethylaminophenyl)-ethylenediamine, 1,2-bis(4-biphenylyl)ethylenediamine, 1,2-bis(1-and 2-naphthyl)ethylenediamines, 1,2-bis(2-furyl)ethylenediamine and l,2-bis(3-pyridyl)ethylenediamine. Although these were all synthesized in the meso form, 1,2-diphenylethylenediamine, 1,2-bis(4-methoxyphenyl)ethylenediamine and 1,2-bis(4-methylphenyl)-ethylenediamine, were all in the DL form. Each DAE solut...

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Methods for the Standardization of Insulin and the Determination of Blood Glucose Concentration

Forst¹,

Hansen²

1975

Insulin

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Eine verbesserung der enzymatischen bestimmung von H2O2. anwendung auf die glucose-bestimmung

Cited by 8 publications

References 5 publications

Use of enzymes in analytical chemistry

Use of enzymes in analytical chemistry

Assay for Peroxidase Using 1,2-DiarylethyIenediamines and Catechol Compounds as Fluorogenic Substrates

Methods for the Standardization of Insulin and the Determination of Blood Glucose Concentration

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