Eine photometrische Methode zur Bestimmung der Phosphatase‐Aktivität in Böden

Hoffmann, Gg.

doi:10.1002/jpln.19681180303

Cited by 92 publications

(16 citation statements)

References 5 publications

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“…Soil acid phosphatase activity was determined after Hoffmann (1967) and Woolhouse (1968). One gram samples of soil were treated with 015-ml amounts of toluene and left for 3 h in stoppered test tubes.…”

Section: Methodsmentioning

confidence: 99%

Floristic Diversity in Relation to Soil Characteristics in a Lead Mining Complex in the Pennines, England

Clark

1981

New Phytologist

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SUMMARYAdjacent species-rich and species-poor areas of a limestone outcrop were investigated in a heavy metal mining complex on Grassington Moor, Yorkshire, England, to determine reasons for the differences in their floras. Levels of soil lead, zinc, nitrogen, phosphorus and potassium were determined and the activities of the enzymes urease, acid phosphatase and dehydrogenase in the soil were measured. Soil respiration rates were also determined. The percentages of the variation in species-density accounted for by each factor were determined using a step-wise multiple regression technique. Binding of lead in organic forms resulting in low levels of available lead, together with higher levels of the major plant nutrients, were probably the main factors enabling a species-rich community to occur on one part of the outcrop. Possible ways in which the differences in habitat became established are discussed.

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Section: Methodsmentioning

confidence: 99%

Floristic Diversity in Relation to Soil Characteristics in a Lead Mining Complex in the Pennines, England

Clark

1981

New Phytologist

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“…Soil suspensions were prepared with 10 ml acetate buffer (pH 5) and 5 ml of 20 mM disodium phenylphosphate (EC 3279-54-7) as substrate solution; in controls, substrate solution was replaced by deionized water. All soil suspensions were incubated at 37°C and continuous shaking (100 rpm) for 3 h. The release of phenol was determined colorimetrically at 614 nm (ELx808, Absorbance Microplate Reader, BioTek Instruments Inc., Winooski, VT, USA), using 2,6-dibromchinone-chlorimide (EC 202-937-2) as coloring reagent (Hoffmann 1968(Hoffmann , modified by Ö hlinger 1996.…”

Section: Determination Of Acid Phosphomonoesterase and Phosphodiestermentioning

confidence: 99%

Soil phosphorus supply controls P nutrition strategies of beech forest ecosystems in Central Europe

et al. 2017

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Phosphorus availability may shape plantmicroorganism-soil interactions in forest ecosystems. Our aim was to quantify the interactions between soil P availability and P nutrition strategies of European beech (Fagus sylvatica) forests. We assumed that plants and microorganisms of P-rich forests carry over mineral-bound P into the biogeochemical P cycle (acquiring strategy). In contrast, P-poor ecosystems establish tight P cycles to sustain their P demand (recycling strategy). We tested if this conceptual model on supply-controlled P nutrition strategies was consistent with data from five European beech forest ecosystems with different parent materials (geosequence), covering a wide range of total soil P stocks (160-900 g P m -2 ; \1 m depth). We analyzed numerous soil chemical and biological properties. Especially P-rich beech ecosystems accumulated P in topsoil horizons in moderately labile forms. Forest floor turnover rates decreased with decreasing total P stocks (from 1/5 to 1/40 per year) while ratios between organic carbon and organic phosphorus (C:P org ) increased from 110 to 984 (A horizons). High proportions of fine-root biomass in forest floors seemed to favor tight P recycling. Phosphorus in fine-root biomass increased relative to microbial P with decreasing P stocks. Concomitantly, phosphodiesterase activity decreased, which might explain increasing proportions of diester-P remaining in the soil organic matter. With decreasing P supply indicator values for P acquisition decreased and those for recycling increased, implying adjustment of plantmicroorganism-soil feedbacks to soil P availability. Intense recycling improves the P use efficiency of beech forests.

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“…Urease activity was analyzed by using a buffered method (Kandeler and Gerber, 1988). Acid phosphatase was determined by the release and detection of pnitrophenol (PNP) using 0.1 M phenylphosphate disodium as substrates (Hoffmann, 1968). β-Glucosidase was determined using salicin as substrate, and the saligenin (SG) released from the substrate reacted with the Gibbes reagent (2,6-dibromchinone-4-chlorimide) to form a blue indophenol dye, which was determined spectrophotometrically at 578 nm and pH 9.0 (Hoffmann and Dedeken, 1965).…”

Section: Microbial Community Function Analysesmentioning

confidence: 99%

Structure and function of microbial communities during the early stages of revegetation of barren soils in the vicinity of a Pb/Zn Smelter

et al. 2006

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Eine photometrische Methode zur Bestimmung der Phosphatase‐Aktivität in Böden

Cited by 92 publications

References 5 publications

Floristic Diversity in Relation to Soil Characteristics in a Lead Mining Complex in the Pennines, England

Floristic Diversity in Relation to Soil Characteristics in a Lead Mining Complex in the Pennines, England

Soil phosphorus supply controls P nutrition strategies of beech forest ecosystems in Central Europe

Structure and function of microbial communities during the early stages of revegetation of barren soils in the vicinity of a Pb/Zn Smelter

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