Eine NMR‐Methode zur Bestimmung der Bindungsreihenfolge supramolekularer Liganden an basische Reste in Proteinen

The interactions of two mono-functionalized sulfonatocalix[4]arenes with cytochrome c were investigated by structural and thermodynamic methods. The replacement of a single sulfonate with either a bromo or a phenyl substituent resulted in altered recognition of cytochrome c as evidenced by X-ray crystallography. The bromo-substituted ligand yielded a new binding mode in which a self-encapsulated calixarene dimer contributed to crystal packing. This ligand also formed a weak halogen bond with the protein. The phenyl-substituted ligand was bound to Lys4 of cytochrome c, in a 1.7 Å resolution crystal structure. A dimeric packing arrangement mediated by ligand-ligand contacts in the crystal suggested a possible assembly mechanism. The different protein recognition properties of these calixarenes are discussed.

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Protein Recognition by Functionalized Sulfonatocalix[4]arenes

Doolan

Rennie

Crowley

2017

Chemistry A European J

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Generation of Dynamic Combinatorial Libraries Using Hydrazone‐Functionalized Surface Mimetics

Hewitt

Wilson

2018

Eur J Org Chem

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Dynamic combinatorial chemistry (DCC) represents an approach, whereby traditional supramolecular scaffolds used for protein surface recognition might be exploited to achieve selective high affinity target recognition. Synthesis, in situ screening and amplification under selection pressure allows the generation of ligands, which bear different moieties capable of making multivalent non‐covalent interactions with target proteins. Generic tetracarboxyphenyl porphyrin scaffolds bearing four hydrazide moieties have been used to form dynamic combinatorial libraries (DCLs) using aniline‐catalyzed reversible hydrazone exchange reactions, in 10 % DMSO, 5 mm NH4OAc, at pH 6.75. High resolution mass spectrometry (HRMS) was used to monitor library composition and establish conditions under which equilibria were established.

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Eine NMR‐Methode zur Bestimmung der Bindungsreihenfolge supramolekularer Liganden an basische Reste in Proteinen

Abstract: Die Erkennung von Proteinoberflächen, die in Protein-Protein-Wechselwirkungen eine Rolle spielen, entwickelt sich zu einem Fokus der supramolekularen Chemie.

Cited by 2 publications

References 38 publications

Protein Recognition by Functionalized Sulfonatocalix[4]arenes

Protein Recognition by Functionalized Sulfonatocalix[4]arenes

Generation of Dynamic Combinatorial Libraries Using Hydrazone‐Functionalized Surface Mimetics

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