Eine Methode zur Prüfung der Resistenz der Kartoffelpflanze gegenüber dem Erreger der Schwarzbeinigkeit (Erwinia carotovora var.atroseptica (van Hall) Dye)

Munzert, Manfred

doi:10.1007/bf02361734

Cited by 6 publications

(4 citation statements)

References 2 publications

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“…The results of the experiments with Ech, however, support the hypothesis that the stem base can form a barrier preventing the mass of bacteria in the rotten tuber tissue to spread to the stem, and that there exists clonal variation in cultivated potato for the extent to which bacteria from rotten tuber tissue succeed in passing this barrier. This has also been concluded by Munzert (1975) and Munzert & Hunnius (1980). It is not clear whether the barrier is of physical nature as suggested by Weber (1990), who ascribed differences between cultivars to differences in the amount and type of lignification in the stem base, or whether it is of biochemical nature, for example, due to the accumulation of antibacterial substances in the sprouts, such as rishitin, a phytoalexin which is produced in response to inoculation with Erwinia and which is bactericidal to Erwinia bacteria in vitro (Lyon, 1989;Abenthum et al, 1995).…”

Section: Discussionsupporting

confidence: 81%

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Components of partial resistance to potato blackleg caused by pectolytic Erwinia carotovora subsp. atroseptica and E. chrysanthemi

et al. 1996

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The various phases of the infection process in potato blackleg, caused by pectolytic Erwinia spp., were interpreted as components of partial resistance and studied in detail. Mother tubers of glasshouse-grown plants of two potato cultivars were inoculated with antibiotic resistant marker strains of Erwinia carotovora subsp. atroseptica (Eca) or E. chrysanthemi (Ech). Stem tissue samples of these plants were collected 3, 7, 14 and 21 days after inoculation and subsequently analysed for the presence of the bacteria. As soon as 3 days after inoculation, Erwinia bacteria were detected in the stem tissue of the plants. However, blackleg developed only when the rotting mother tuber tissue reached the sites where the stems were attached to the mother tuber. An inoculation method was developed for determining cultivar differences in the incidence and rate at which rot in tuber cylinders proceeds to sprouts on top of such cylinders. A set of 12 cultivars was screened for this putative component of resistance which was called 'stem base resistance'. Significant differences for stem base resistance were found among the cultivars. These differences were clearer when inoculation was carried out with Ech in contrast to Eca. Glasshouse-grown plants of the same set of cultivars were also screened for resistance of the above ground part of the stem. Cultivars differed significantly for this so-called 'stem tissue resistance', but results of some cultivars were not consistent among years of testing. Multiple regression analysis revealed that stem base resistance, stem tissue resistance, tuber tissue resistance and resistance of mother tubers in the field account for 63% (Eca) and 75% (Ech) of the variance found after screening for resistance to blackleg in the field. It was concluded that if results of this study are extrapolated to any set of potato clones, selection for resistance in breeding programmes under laboratory or glasshouse conditions, would be most efficient when directed to clonal differences for stem base resistance.

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Section: Discussionsupporting

confidence: 81%

“…Another component of resistance may be located in the stem base, as suggested by Munzert (1975) and Munzert & Hunnius (1980), who screened fully grown plants in the glasshouse by inoculating the seed piece from which they were grown. After incubation, the amount of seed piece decay varied among genotypes.…”

Section: Introductionmentioning

confidence: 99%

Components of partial resistance to potato blackleg caused by pectolytic Erwinia carotovora subsp. atroseptica and E. chrysanthemi

et al. 1996

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“…Methods are only suitable if they are easy to perform, applicable to early vegetative generations and yield reproducible and quantitative data that show good agreement with the resistance of the clones in farmers' fields. Numerous methods for screening of plants in the field [Hossain and Logan, 1983;Lapwood and Legg, 1983;Lapwood and Gans, 1984;Lapwood and Read, 1986a;Gans et al, 1991], or in the glasshouse [Munzert, 1975;Hidalgo and Echandi, 1982;Lapwood and Read, 1986b;Lojkowska and Kelman, 1989] have been described. Field tests correspond most to field conditions but they are expensive to perform and not feasible when only a small amount of seed is available.…”

Section: Introductionmentioning

confidence: 99%

The role of the seed tuber in determining partial resistance to potato blackleg caused byErwinia spp.

Allefs¹,

Dooijeweert

Jong

et al. 1995

Eur J Plant Pathol

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In t991 and 1992, 12 potato cultivars were screened at two locations for resistance to blackleg, after vacuum infiltration of the seed with Erwinia carotovora subsp, atroseptica or E. chrysanthemi. Cultivar differences for resistance to E.c. subsp, atroseptica and E. chrysanthemi were found which were consistent over locations and years. Seed tubers of the same cultivars were also screened for resistance to both Erwinia spp. by using a tuber slice inoculation method. Correlation coefficients for comparisons between resistance to blackleg in the field and tuber tissue resistance under aerobic or anaerobic conditions were not significant. This could partly be explained by drastic changes in relative tuber tissue resistance of the cultivars within a 5 weeks period after planting in the field. Presprouting of seed tubers in diffuse daylight had a less pronounced effect on relative tuber tissue resistance than planting in the field. Monitoring the process of mother tuber decay during the growing season of 1993 after vacuum infiltration with E.c. subsp, atroseptica and E. chrysanthemi revealed that cultivars differed in the extent to which these bacteria enhanced the process of mother tuber decay. These differences partly explained the cultivar differences for resistance to blackleg in the field.Abbreviations: Eca = Erwinia carotovora subsp, atroseptica; Ech = Erwinia chrysanthemi; NOP = Noordoostpolder; Wag --Wageningen

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“…The method described for the first time by Muenzert (1975), was modified by Pietrak (2001), by using a toothpick for inoculation instead of blotting paper. Tubers are a source of eye-cuts, from which single stem plants are grown in pots (of diameter 8 cm).…”

Section: A Evaluation Of Stem Tissue Resistance In Greenhouse Growinmentioning

confidence: 99%

Screening for Potato Resistance to Blackleg and Soft Rot

Lebecka

2017

Plant Breeding and Seed Science

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Two diseases of the potato, blackleg of potato plants and soft rot of tubers, are caused by several species of pectinolytic bacteria which belong to two genera: Pectobacterium and Dickeya. Resistance to these bacteria is polygenic and the expression of resistance in tubers and plants is only partially related, as well as strongly dependent on the aggressiveness of the bacteria and on environmental factors. Two methods of assessing tuber and stem tissue resistance of potato cultivars and breeding lines are described.

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Eine Methode zur Prüfung der Resistenz der Kartoffelpflanze gegenüber dem Erreger der Schwarzbeinigkeit (Erwinia carotovora var.atroseptica (van Hall) Dye)

Cited by 6 publications

References 2 publications

Components of partial resistance to potato blackleg caused by pectolytic Erwinia carotovora subsp. atroseptica and E. chrysanthemi

Components of partial resistance to potato blackleg caused by pectolytic Erwinia carotovora subsp. atroseptica and E. chrysanthemi

The role of the seed tuber in determining partial resistance to potato blackleg caused byErwinia spp.

Screening for Potato Resistance to Blackleg and Soft Rot

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