Eine Methode zur colorimetrischen Bestimmung der β-Glucosidase-Aktivität in Böden

Hoffmann, Gg.; Dedeken, M.

doi:10.1002/jpln.19651080302

Cited by 47 publications

(17 citation statements)

References 2 publications

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“…β-Glucosidase activity was measured using β-glucosido-saligenin as substrate (Strobl and Traunmüller 1996) by a modification of the method of Hoffmann and Dedeken (1965). Soil samples were incubated with the substrate for 3 h at 37 o C. Saligenin released from the substrate was determined colorimetrically.…”

Section: Soil Measurement Methodologiesmentioning

confidence: 99%

Soil Quality Dynamics Following Long-Term Application of Poultry Manure and Sewage Sludge on Grassland

Mariscal‐Sancho

Ball

Peregrina

2011

Communications in Soil Science and Plant Analysis

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Section: Soil Measurement Methodologiesmentioning

confidence: 99%

Soil Quality Dynamics Following Long-Term Application of Poultry Manure and Sewage Sludge on Grassland

Mariscal‐Sancho

Ball

Peregrina

2011

Communications in Soil Science and Plant Analysis

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“…b-Glucosidase was determined using b-glucosido-salicin as substrate (Hoffmann and Dedeken, 1965). After incubation for 3 h at 37°C, saligenin released from the substrate was determined colorimetrically at 578 nm after coloring with 2,6-dib- Drijber et al, 2000;Grogan and Cronan, 1997) VAM fungi 16:1x5c (Olsson and Alstro¨m, 2000) Actinomycetes 10Me16:0, 10Me17:0, 10Me18:0 (Frostega˚rd et al, 1993b;Kourtev et al, 2002a;Kroppenstedt, 1985Kroppenstedt, , 1992) Monounsaturated 16:1x7t, 17:1x8c, 18:1x7c, 16:1x5c Vestal and White, 1989) Saturated 10:0, 12:0, 13:0, 14:0, 15:0, 17:0, 16:0, 18:0, 20:0 Vestal and White, 1989) Mono/sat 16:1x7t + 17:1x8c + 18:1x7c + 16:1x5c / 10:0 + 12:0 + 13:0 + 14:0 + 15:0 + 16:0 + 17:0 + 18:0 + 20:0 Zelles et al, 1992 and romchinone-4-chlorimide.…”

Section: Microbial Community Functionmentioning

confidence: 99%

Changes in Soil Microbial Community Associated with Invasion of the Exotic Weed, Mikania micrantha H.B.K

et al. 2006

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Invasions of exotic plant species are among the most pervasive and important threats to natural ecosystems. However, the effects of plant invasions on soil processes and soil biota have not been adequately investigated. Changes were studied in soil microbial communities where Mikania micrantha was invading a native forest community in Neilingding Island, Shenzhen, China. The soil microbial community structure (assessed by phospholipid fatty acid [PLFA] profiles) and function (assessed by enzyme activities), as well as soil chemical properties were measured. The results showed that the invasion of M. micrantha into the evergreen broadleaved forests in South China changed most of the characteristics in studied soils. Microbial community structure and function differed significantly among the native, two ecotones, and exotic-derived soils. For PLFA profiles, we observed a significant increase in aerobic bacteria but a decrease in anaerobic bacteria in the M. micrantha monoculture as compared to the native and ecotones. The ratio of cy19:0 to18:1x7 gradually declined but mono/sat PLFAs increased as M. micrantha became more dominant. Both ratios were significantly related to pH according to regression analysis, therefore, pH was a sensitive indicator reflecting the invaded soil subsystem succession. The microbial community composition clearly separated the native soil from the invaded soils by principal component analysis (PCA) and discriminant analysis (DA). For enzyme activities, 7 of 9 enzymes (b-glucosidase, invertase, protease, urease, acid phosphatase, alkaline phosphatase, and phenol oxidase) showed the similar trend that the activities were highest in the exotic, intermediate in the two ecotones, and lowest in the native community. In most cases, enzyme activities were influenced by soil chemical properties, especially by pH value and soil organic matter. Differences in the structural variables were well correlated to differences in the functional variables as demonstrated by canonical correlation analysis (CCA). It was concluded that M. micrantha invasion had profound effects on the soil subsystem, which must be taken into account when we try to control its invasions.

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“…β-glucosidase (EC 3.2.1.21) activity was determined according to Hoffman and Dedeken [16], among others [17,18]. For each soil sample, two subsamples and one control (without substrate) of 5 g of field-moist soil (<2 mm) were incubated for 3 h at 37 • C in a buffered solution (acetate buffer 2 M with pH 6.2), using 2-(hydroxymethyl)phenyl-β-D-glucopyranoside (salicin) as a substrate.…”

Section: Measured Variablesmentioning

confidence: 99%

Influence of Tillage Practices, Organic Manures and Extrinsic Factors on β-Glucosidase Activity: The Final Step of Cellulose Hydrolysis

2018

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β-glucosidase is a key enzyme in the C-cycle, and its activity is strongly influenced by soil management practices and by extrinsic factors such as weather conditions. However, the variability of β-glucosidase activity (BGa) over time and how this variability is affected by tillage and organic fertilization remain poorly elucidated. We investigated how contrasting tillage practices (zero, minimum, conventional or deep) and organic amendment application (dairy cattle slurry or green compost) govern BGa. Strong correlations (r = −0.98; p < 0.001) were found between BGa and preceding cumulative rainfall for soil without crops. Under cropped soil conditions, BGa was 53% greater under zero and minimum tillage than under ploughed treatments (p < 0.05) six years after their applications. These differences could be explained by associated effects of tillage on organic matter content, electrical conductivity and water stable aggregates. The separated organic residue experiment showed that BGa was 36% greater in soil amended with slurry than in soil amended with compost (p < 0.05) five years after their applications. Finally, the response ratio of BGa was calculated in order to study the effect size of each treatment at each sampling period. This simple strategy mitigated the variation on BGa measurements between two sampling periods, and may be applied in future studies to improve the interpretation of other potential soil quality indicators that are sensitive to seasonal changes.

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Eine Methode zur colorimetrischen Bestimmung der β-Glucosidase-Aktivität in Böden

Cited by 47 publications

References 2 publications

Soil Quality Dynamics Following Long-Term Application of Poultry Manure and Sewage Sludge on Grassland

Soil Quality Dynamics Following Long-Term Application of Poultry Manure and Sewage Sludge on Grassland

Changes in Soil Microbial Community Associated with Invasion of the Exotic Weed, Mikania micrantha H.B.K

Influence of Tillage Practices, Organic Manures and Extrinsic Factors on β-Glucosidase Activity: The Final Step of Cellulose Hydrolysis

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