Eine einfache Technik der extrem schnellen Abk�hlung gr��erer Gewebest�cke

Wollenberger, A; Ristau, O.; Schoffa, G.

doi:10.1007/bf00362995

Cited by 990 publications

(243 citation statements)

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“…The perfusate contained either 10 -s M glucagon (Eli Lilly Co.), 50 mM glucose or no addition (control) and the perfusion continued for 15 min after which time the livers were freeze-clamped [26]. The sugar-P's were then isolated as their Ba 2÷ salts from 40 g (wet wt) tissue (pooled from separate liver perfusions) from each experimental condition [2].…”

Section: Methodsmentioning

confidence: 99%

Regulation of hepatic altro heptulose 1,7‐bisphosphate levels and control of flux through the pentose pathway by fructose 2,6‐bisphosphate

Blackmore

Shuman

1982

FEBS Letters

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Section: Methodsmentioning

confidence: 99%

Regulation of hepatic altro heptulose 1,7‐bisphosphate levels and control of flux through the pentose pathway by fructose 2,6‐bisphosphate

Blackmore

Shuman

1982

FEBS Letters

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“…During the linear part of the time-course of glycolysis or gluconeogenesis, the renal vessels were cut and the kidney was rapidly clamped between aluminium tongs cooled in liquid N2 (Wollenberger, Ristau & Schoffa, 1960). Delay between cutting the vessels and freezing was 2-3see.…”

Section: Methodsmentioning

confidence: 99%

Effects of added adenine nucleotides on renal carbohydrate metabolism

Weidemann

Hems

Krebs

1969

Biochemical Journal

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1. The regulatory effects that adenine nucleotides are known to exert on enzymes of glycolysis and gluconeogenesis were demonstrated to operate in kidney-cortex slices and in the isolated perfused rat kidney by the addition of exogenous ATP, ADP and AMP to the incubation or perfusion media. 2. Both preparations rapidly converted added ATP into ADP and AMP, and ADP into AMP; added AMP was rapidly dephosphorylated. AMP formed from ATP was dephosphorylated at a lower rate than was added AMP, especially when the initial ATP concentration was high (10mM). Deamination ofadded AMP occurred more slowly than dephosphorylation of AMP. 3. Gluconeogenesis from lactate or propionate by rat kidney-cortex slices, and from lactate by the isolated perfused rat kidney, was inhibited by the addition of adenine nucleotides to the incubation or perfusion media. In contrast, oxygen consumption and the utilization of propionate or lactate by slices were not significantly affected by added ATP or AMP. 4. The extent and rapidity of onset of the inhibition of renal gluconeogenesis were proportional to the AMP concentration in the medium and the tissue, and were not due to the production of acid or Pi or the formation of complexes with Mg2+ ions. 5. Glucose uptake by kidney-cortex slices was stimulated 30-50% by added ATP, but the extra glucose removed was not oxidized to carbon dioxide and did not all appear as lactate. Glucose uptake, but not lactate production, by the isolated perfused kidney was also stimulated by the addition of ATP or AMP. 6. In the presence of either glucose or lactate, ATP and AMP greatly increased the concentrations of C3 phosphorylated intermediates and fructose 1,6-diphosphate in the kidney. There was a simultaneous rise in the concentration of malate and fall in the concentration of a-oxoglutarate. 7. The effects of added adenine nucleotides on renal carbohydrate metabolism seem to be mainly due to an increased concentration of intracellular AMP, which inhibits fructose diphosphatase and deinhibits phosphofructokinase. This conclusion is supported by the accumulation of intermediates of the glycolytic pathway between fructose diphosphate and pyruvate. 8. ATP or ADP (10mM) added to the medium perfusing an isolated rat kidney temporarily increased the renal vascular resistance, greatly diminishing the flow rate of perfusion medium for a period of several minutes.

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“…Control livers were infused with 0.15 M NaCI in place of the insulin solution. At the end of perfusion portions of the liver were taken by a freeze-stop technique [10] and stored under liquid N2. The same liver lobe was always sampled.…”

Section: Methodsmentioning

confidence: 99%