Eine aktivierbare Lanthanoid‐Lumineszenzsonde für die zeitgesteuerte Detektion von Nitroreduktase in lebenden Bakterien

Brennecke, Benjamin; Wang, Qinghua; Zhang, Qingyang; Hu, Hai‐Yu; Nazaré, Marc

doi:10.1002/ange.202002391

Cited by 9 publications

(1 citation statement)

References 35 publications

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“…Since TCyIÀ OH has a higher fluorescence quantum yield than CyIÀ OH, thus the precursor TCyIÀ NO 2 was selected as the representative in this study. Incubation of TCyIÀ NO 2 with S. aureus lysate, which is rich in NTR, [24] resulted in a significant increase in fluorescence intensity (Figure 2A). The fluorescence increase was abated to a certain extent by addition of dicoumarol (100 μM), confirming the crucial role of NTR in this process.…”

Section: Resultsmentioning

confidence: 99%

Nitroreductase‐Responsive Photosensitizers for Selective Imaging and Photo‐Inactivation of Intracellular Bacteria

Zhou,

Da,

Jian

et al. 2024

Chemistry A European J

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Intracellular Staphylococcus aureus (S. aureus), especially the methicillin resistant staphylococcus aureus (MRSA), are difficult to detect and eradicate due to the protection by the host cells. Antibacterial photodynamic therapy (aPDT) offers promise in treating intracellular bacteria, provided that selective damage to the bacteria ranther than host cells can be realized. According to the different nitroreductase (NTR) levels in mammalian cells and S. aureus, herein NTR‐responsive photosensitizers (PSs) (T)CyI‐NO2 were designed and synthesized. The emission and 1O2 generation of (T)CyI‐NO2 are quenched by the 4‐nitrobenzyl group, but can be specifically switched on by bacterial NTR. Therefore, selective imaging and photo‐inactivation of intracellular S. aureus and MRSA were achieved. Our findings may pave the way for the development of more efficient and selective aPDT agents to combat intractable intracellular infections.

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Section: Resultsmentioning

confidence: 99%

Nitroreductase‐Responsive Photosensitizers for Selective Imaging and Photo‐Inactivation of Intracellular Bacteria

Zhou,

Da,

Jian

et al. 2024

Chemistry A European J

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A General Approach to Design Dual Ratiometric Fluorescent and Photoacoustic Probes for Quantitatively Visualizing Tumor Hypoxia Levels In Vivo

et al. 2021

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Herein, we describe an energy balance strategy between fluorescence and photoacoustic effects by sulfur substitution to transform existing hemicyanine dyes (Cy) into optimized NIRF/PA dual ratiometric scaffolds. Based on this optimized scaffold, we reported the first dual-ratio response of nitroreductase probe AS-Cy-NO 2 , which allows quantitative visualization of tumor hypoxia in vivo. AS-Cy-NO 2 , composed of a new NIRF/PA scaffold thioxanthene-hemicyanine (AS-Cy-1) and a 4-nitrobenzene moiety, showed a 10-fold ratiometric NIRF enhancement (I 773 /I 733 ) and 2.4-fold ratiometric PA enhancement (PA 730 /PA 670 ) upon activation by a biomarker (nitroreductase, NTR) associated with tumor hypoxia. Moreover, the dual ratiometric NIRF/PA imaging accurately quantified the hypoxia extent with high sensitivity and high imaging depth in xenograft breast cancer models. More importantly, the 3D maximal intensity projection (MIP) PA images of the probe can precisely differentiate the highly heterogeneous oxygen distribution in solid tumor. Thus, this study provides a promising NIRF/PA scaffold that may be generalized for the dual ratiometric imaging of other disease-relevant biomarkers.

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In Vivo Nitroreductase Imaging via Fluorescence and Chemical Shift Dependent ¹⁹F NMR

Chen

Xiao

et al. 2022

Angewandte Chemie

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Nitroreductase (NTR) is an important biomarker widely used to evaluate the degree of tumor hypoxia. Although a few optical methods have been reported for detecting nitroreductase at low concentration ranges, an effective strategy for nitroreductase monitoring in vivo without limits to the imaging depth is still lacking. Herein, a novel dual-mode NIR fluorescence and 19 F MRI agent, FCy7-NO 2 , is proposed for imaging tumor hypoxia. We show that FCy7-NO 2 serves as not only a rapid NIR fluorescence enhanced probe for monitoring and bioimaging of nitroreductase in tumors, but also a novel 19 F MR chemical shiftsensitive contrast agent for selectively detecting nitroreductase catalyzed reduction. Notably, integrating two complementary imaging technologies into FCy7-NO 2 enables sensitive detection of nitroreductase in a broad concentration range without tissue-depth limit. In general, this agent has a remarkable response to nitroreductase, which provides a promising method for understanding tumor evolution and its physiological role in the hypoxic microenvironment.

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Eine aktivierbare Lanthanoid‐Lumineszenzsonde für die zeitgesteuerte Detektion von Nitroreduktase in lebenden Bakterien

Cited by 9 publications

References 35 publications

Nitroreductase‐Responsive Photosensitizers for Selective Imaging and Photo‐Inactivation of Intracellular Bacteria

Nitroreductase‐Responsive Photosensitizers for Selective Imaging and Photo‐Inactivation of Intracellular Bacteria

A General Approach to Design Dual Ratiometric Fluorescent and Photoacoustic Probes for Quantitatively Visualizing Tumor Hypoxia Levels In Vivo

In Vivo Nitroreductase Imaging via Fluorescence and Chemical Shift Dependent ¹⁹F NMR

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Eine aktivierbare Lanthanoid‐Lumineszenzsonde für die zeitgesteuerte Detektion von Nitroreduktase in lebenden Bakterien

Cited by 9 publications

References 35 publications

Nitroreductase‐Responsive Photosensitizers for Selective Imaging and Photo‐Inactivation of Intracellular Bacteria

Nitroreductase‐Responsive Photosensitizers for Selective Imaging and Photo‐Inactivation of Intracellular Bacteria

A General Approach to Design Dual Ratiometric Fluorescent and Photoacoustic Probes for Quantitatively Visualizing Tumor Hypoxia Levels In Vivo

In Vivo Nitroreductase Imaging via Fluorescence and Chemical Shift Dependent 19F NMR

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In Vivo Nitroreductase Imaging via Fluorescence and Chemical Shift Dependent ¹⁹F NMR