EIN3-Dependent Regulation of Plant Ethylene Hormone Signaling by Two Arabidopsis F Box Proteins

Potuschak, Thomas; Lechner, Esther; Parmentier, Yves; Yanagisawa, Shuichi; Grava, Sandrine; Koncz, Csaba; Genschik, Pascal

doi:10.1016/s0092-8674(03)00968-1

Cited by 636 publications

(612 citation statements)

References 39 publications

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“…Therefore, the interaction between MED25 and the two crucial components (EIN3/EIL1) in the ethylene signaling pathway may affect ethylene-mediated plant growth (Alonso et al, 2003;Guo and Ecker, 2003;Potuschak et al, 2003). The med25 mutant showed late flowering and abnormal root hair phenotypes (Cerdan and Chory, 2003;Sundaravelpandian et al, 2013), consistent with the proposal that ethylene signaling is involved in multiple developmental processes including flowering and root hair development (Pitts et al, 1998;Dolan, 2001;Ogawara et al, 2003).…”

Section: Discussionsupporting

confidence: 74%

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

et al. 2014

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These authors contributed equally to this work. SUMMARYIron is an essential micronutrient for plants and animals, and plants are a major source of iron for humans. Therefore, understanding the regulation of iron homeostasis in plants is critical. We identified a T-DNA insertion mutant, yellow and sensitive to iron-deficiency 1 (yid1), that was hypersensitive to iron deficiency, containing a reduced amount of iron. YID1 encodes the Arabidopsis Mediator complex subunit MED16. We demonstrated that YID1/MED16 interacted with another subunit, MED25. MED25 played an important role in regulation of iron homeostasis by interacting with EIN3 and EIL1, two transcription factors in ethylene signaling associated with regulation of iron homeostasis. We found that the transcriptome in yid1 and med25 mutants was significantly affected by iron deficiency. In particular, the transcription levels of FIT, IRT1 and FRO2 were reduced in the yid1 and med25 mutants under iron-deficient conditions. The finding that YID1/MED16 and MED25 positively regulate iron homeostasis in Arabidopsis increases our understanding of the complex transcriptional regulation of iron homeostasis in plants.

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Section: Discussionsupporting

confidence: 74%

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

et al. 2014

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“…This phenotype is extremely strong although the amiR-ETP1/ETP2 plants are slightly larger than their ctr1 counterpart (data not shown). Strikingly, knocking down ETP1 and ETP2 expression also stunted growth and renders the plants partially sterile, which is similar to the phenotypes observed in the etr1/etr2/ein4/ ers2 quadruple mutant (Hua et al 1998) and the ebf1 ebf2 double mutant (Guo and Ecker 2003;Potuschak et al 2003). In contrast, overexpression of ETP1 and ETP2 results in plants manifesting an ethylene-insensitive phenotype.…”

Section: Ein2 C-terminal End Is Of Crucial Importance To Ethylene Ressupporting

confidence: 59%

“…In plants, there are ;700 F-box proteins (Gagne et al 2002), and they are involved in a plethora of biological processes including plant hormone responses (Xie et al 1998;Gray et al 2001;Guo and Ecker 2003;Potuschak et al 2003;Fu et al 2004;Dharmasiri et al 2005;Kepinski and Leyser 2005), lateral root formation (Coates et al 2006), light signaling and clock control (Mas et al 2003;Imaizumi et al 2003Imaizumi et al , 2005, pollen recognition and rejection (Qiao et al 2004a,b;Sijacic et al 2004), and plant-pathogen interactions (Kim and Delaney 2002;Duyvesteijn et al 2005;van den Burg et al 2008). Based on phylogenetic analysis, the superfamily of F-box proteins is divided into 20 groups (Gagne et al 2002).…”

Section: Ein2 C-terminal End Is Of Crucial Importance To Ethylene Resmentioning

confidence: 99%

“…Additionally, a number of groups found that post-transcriptional regulation of protein levels is a key mechanism of modulating EIN3 activity by ethylene. Specifically, they found that ubiquitin/proteasomemediated degradation negatively regulates ethylene responses by targeting EIN3 for turnover through two F-box proteins, EIN3-BINDING F-BOX PROTEIN1 (EBF1) and EIN3-BINDING F-BOX PROTEIN2 (EBF2) (Guo and Ecker 2003;Potuschak et al 2003;Gagne et al 2004). Interestingly, negative feedback regulation exists in this step of the ethylene signal-transduction pathway, in that EIN3 targets the promoter of EBF2 to control its expression level likely allowing fine-tuning of ethylene responses (Binder et al 2007;Konishi and Yanagisawa 2008).…”

mentioning

confidence: 99%

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Interplay between ethylene, ETP1/ETP2 F-box proteins, and degradation of EIN2 triggers ethylene responses in Arabidopsis

et al. 2009

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The gaseous plant hormone ethylene can trigger myriad physiological and morphological responses in plants. While many ethylene signaling pathway components have been identified and characterized, little is known about the function of the integral membrane protein ETHYLENE-INSENSITIVE2 (EIN2), a central regulator of all ethylene responses. Here, we demonstrate that Arabidopsis thaliana EIN2 is a protein with a short half-life that undergoes rapid proteasome-mediated protein turnover. Moreover, EIN2 protein accumulation is positively regulated by ethylene. We identified two F-box proteins, EIN2 TARGETING PROTEIN1 (ETP1) and EIN2 TARGETING PROTEIN2 (ETP2), that interact with the EIN2 C-terminal domain (EIN2-CEND), which is highly conserved and sufficient to activate most ethylene responses. Overexpression of ETP1 or ETP2 disrupts EIN2 protein accumulation, and these plants manifest a strong ethylene-insensitive phenotype. Furthermore, knocking down the levels of both ETP1 and ETP2 mRNAs using an artificial microRNA (amiRNA) leads to accumulation of EIN2 protein, resulting in plants that display constitutive ethylene response phenotypes. Finally, ethylene downregulates ETP1 and ETP2 proteins, impairing their ability to interact with EIN2. Thus, these studies reveal that a complex interplay between ethylene, the regulation of ETP1/ETP2 F-box proteins, and subsequent targeting and degradation of EIN2 is essential for triggering ethylene responses in plants.[Keywords: Hormone; protein degradation; signal transduction; plant] Supplemental material is available at http://www.genesdev.org.

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“…EIN3 is a transcription factor that plays a crucial role in regulation of expression of ethylene responsive genes (Chao et al 1997;Solano et al 1998). Studies have also revealed that in absence of ethylene EIN3 protein is quickly degraded through a ubiquitin-proteasome pathway mediated by two F-box proteins, [EIN3-binding F box protein 1 and 2 (EBF1 and EBF2)] whereas EIN3 protein is stabilized by ethylene itself (Guo and Ecker 2003;Potuschak et al 2003;Gagne et al 2004). Recent work on apple by Johnston et al (2009) has revealed that early and late ripening events differs not only in their dependence on ethylene but also towards their sensitivity to ethylene.…”

Section: Role Of Ethylene In Regulation Of Fruit Ripeningmentioning

confidence: 99%

The fading distinctions between classical patterns of ripening in climacteric and non-climacteric fruit and the ubiquity of ethylene—An overview

2011

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The process of fruit ripening is normally viewed distinctly in climacteric and non-climacteric fruits. But, many fruits such as guava, melon, Japanese plum, Asian pear and pepper show climacteric as well as non-climacteric behaviour depending on the cultivar or genotype. Investigations on in planta levels of CO 2 and ethylene at various stages of fruits during ripening supported the role and involvement of changes in the rate of respiration and ethylene production in non-climacteric fruits such as strawberry, grapes and citrus. Non-climacteric fruits are also reported to respond to the exogenous application of ethylene. Comparative analysis of plant-attached and plant-detached fruits did not show similarity in their ripening behaviour. This disparity is being explained in view of 1. Hypothetical ripening inhibitor, 2. Differences in the production, release and endogenous levels of ethylene, 3. Sensitivity of fruits towards ethylene and 4. Variations in the gaseous microenvironment among fruits and their varieties. Detailed studies on genetic and inheritance patterns along with the application of '-omics' research indicated that ethylene-dependent and ethylene-independent pathways coexist in both climacteric and non-climacteric fruits. Auxin levels also interact with ethylene in regulating ripening. These findings therefore reveal that the classification of fruits based on climacteric rise and/or ethylene production status is not very distinct or perfect. However, presence of a characteristic rise in CO 2 levels and a burst in ethylene production in some non-climacteric fruits as well as the presence of system 2 of ethylene production point to a ubiquitous role for ethylene in fruit ripening.

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EIN3-Dependent Regulation of Plant Ethylene Hormone Signaling by Two Arabidopsis F Box Proteins

Cited by 636 publications

References 39 publications

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

Interplay between ethylene, ETP1/ETP2 F-box proteins, and degradation of EIN2 triggers ethylene responses in Arabidopsis

The fading distinctions between classical patterns of ripening in climacteric and non-climacteric fruit and the ubiquity of ethylene—An overview

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