Ein Modell zur langfristigen parenteralen Ernährung der Ratte

Brenner, U.; Müller, Jochen; Keller, H.; Walter, M.

doi:10.1159/000221660

Cited by 2 publications

(2 citation statements)

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“…The total length of the infusion line was 1·5 m. During parenteral nutrition the rats gained weight and were in excellent general health. The cumulative nitrogen-balance was positive with a 95% retention (Brenner, Muller, Keller & Walter, 1984).…”

Section: Animal Carementioning

confidence: 92%

“…Several publications deal with the composition of i.v. nutrition and have proved their effectiveness (Birkhahn, Bellinger & Border, 1976;Brenner, Muller, Keller & Walter, 1984;Dalton, Tourraine & Wilson, 1969;Daly, Reynolds, Rowlands, Dudrick & Copeland, 1980;Davis, 1966;Jones & Hynd, 1981; Neuhauser, Gottman & Bassler, 1984; OramSmith, Stein, Wallace & Muller, 1977;Steiger, Vars & Dudrick, 1972;Stein et al, 1976;Sitren, Fisher & Ali, 1975;Terkel, 1972;Weeks & Davis 1964). This paper describes a catheter system for long-term intravenous infusion which is both inexpensive 'and simple to construct.…”

Section: Abstract: Rat; Intravenous Infusion; Parenteral Nutritionmentioning

confidence: 97%

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A catheter system for long-term intravenous infusion in unrestrained rats

et al. 1985

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SummaryAn intravenous catheter system for long-term (at least 6-8 weeks) parenteral nutrition of unrestrained rats is described. The apparatus is inexpensive and can be constructed from commonly available materials. Keywords: Rat; Intravenous infusion; Parenteral nutritionFor investigations in the field of experimental parenteral nutrition and in particular for investigating tumour growth using different forms of nutrition, the rat-model described by Steiger, Vars & Dudrick (1972) has proved valuable. A disadvantage is that the method is quite elaborate and in particular that the stainless steel support for the catheter and the canvas harness are rather complicated. Several publications deal with the composition of i.v. nutrition and have proved their effectiveness (Birkhahn, Bellinger & Border, 1976;Brenner, Muller, Keller & Walter, 1984;Dalton, Tourraine & Wilson, 1969;Daly, Reynolds, Rowlands, Dudrick & Copeland, 1980;Davis, 1966;Jones & Hynd, 1981; Neuhauser, Gottman & Bassler, 1984; OramSmith, Stein, Wallace & Muller, 1977;Steiger, Vars & Dudrick, 1972;Stein et al., 1976;Sitren, Fisher & Ali, 1975;Terkel, 1972;Weeks & Davis 1964). This paper describes a catheter system for long-term intravenous infusion which is both inexpensive 'and simple to construct. Materials and methodsAnimals 3-month-old male Sprague-Dawley rats, weighing approximately 400--500 g were obtained from Dr Ivanovas GmbH, KisslegglAlIgau, FRG. Catheter assemblyThe finger supports were removed from a plastic tuberculin syringe and 4 holes drilled as shown in Fig. l(E). The barrel of a second tuberculin syringe was shortened to a length of 5 cm and 4 holes Received 12 November 1984. Accepted 18 February 1985 pierced in the finger supports on either side of the syringe barrel (Fig. l(B». A 16-cm length of silicone rubber tubing (Fig. 1(0)) (10 7 mm, 00 1·2mm) (Silastic: Dow Corning Corporation, Medical Products, USA) was fixed to the tip of a 22-G 'Abbocath' (Fig. 1(C) (Abbott Ireland Ltd, Sligo, Republic of Ireland) flexible cannula with cyanoacrylate glue. The luer mount of the 'Abbocath' was trimmed so that it could be inserted into the barrel of the tuberculin syringe and anchored in place with cyanoacrylate glue (Fig. l(A» Implantation procedureThe rats were anaesthetized with ether. Under aseptic conditions a I-em long incision was made between the mandibular angle and the sternum, and the external jugular vein exposed by blunt dissection. The vein was dissected free of the surrounding connective tissue, and ligatures passed beneath it to aid exposure. A subcutaneous tunnel was formed anteriorly to the incision, to accommodate a loop of the catheter (Fig. 2), and the tunnel was then extended dorsally subcutaneously to the back of the neck. A second skin incision was then made, 1 em long and running across the neck. A subcutaneous pouch was produced by blunt dissection .of the margins of the neck skin incision, to enable subcutaneous placement of the finger support (see Fig. 2). The free end of the catheter was grasped by a small pair o...

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Section: Animal Carementioning

confidence: 92%

Section: Abstract: Rat; Intravenous Infusion; Parenteral Nutritionmentioning

confidence: 97%

A catheter system for long-term intravenous infusion in unrestrained rats

et al. 1985

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A new device for long-term continuous enteral nutrition of rats by elementary diet via gastrostomy, following extensive oesophageal or lower gastrointestinal surgery

Müller

Schaarschmidt

Stratmann³

1992

Lab Anim

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SummaryA device for intragastric nutrition of unsedated and minimally restrained rats under complete alimentary abstinence has been developed. The cannulation system consists of an infusion pump, modified glass syringe as flow swivel, rat-harness and a silicone-tube-gastrostomy. The animals were kept in specialcagesand coprophagy was prevented by an own model of faecal collection cup.Methionine and Ca-glycerophosphate had to be added to a commercial elementary diet. The rats were allowed to move freely during intragastric infusion, which was applied for 14 to 28 days in 118 Wistar-rats (350-400 g). They maintained weight on an energy supply of 86·4 kcal/ day. Enteral tube-nutrition by elementary diets has gained increasing importance for postoperative management after gastrointestinal surgery in human medicine and since the early seventies has replaced total parenteral nutrition in many cases, where total alimentary abstinence is necessitated by the operation, Keywords

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Ein Modell zur langfristigen parenteralen Ernährung der Ratte

Cited by 2 publications

References 0 publications

A catheter system for long-term intravenous infusion in unrestrained rats

A catheter system for long-term intravenous infusion in unrestrained rats

A new device for long-term continuous enteral nutrition of rats by elementary diet via gastrostomy, following extensive oesophageal or lower gastrointestinal surgery

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