Eimeria tenella: Utilization of a nasal vaccine with sporozoite antigens incorporated into Iscom as protection for broiler breeders against a homologous challenge

Garcia, João Luís; Guimarães, José da Silva; Headley, Selwyn Arlington; Bogado, Alexey Leon Gomel; Bugni, Felipe Monteiro; Ramalho, Daniela Cristina; Souza, Leonardo Montemor de

doi:10.1016/j.exppara.2008.07.007

Cited by 14 publications

(15 citation statements)

References 32 publications

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“…Although a low pathogenic challenge was used, the histopathological findings observed during this study demonstrated lesions (increase of the regeneration of superficial epithelium and epithelial cell hyperplasia, as well as decrease in the levels of hemorrhage, tissue necrosis, and inflammatory influx) indicative of decreased severity of infection due to protection produced by the T2 and T4 protocols when compared with controls. Similar histopathological findings were described (GARCIA et al, 2008), but with reduced manifestation of epithelial cell hyperplasia.…”

Section: Discussionsupporting

confidence: 71%

“…When the elimination of oocysts was evaluated one week after challenge, the vaccine containing infective oocysts offered greater protection to the birds relative to that with sporozoite proteins, resulting, in a reduction of 99.9% and 12.2%, respectively. Garcia et al (2008) evaluated a nasal vaccine containing sporozoites antigens of E. tenella associated with ISCOM adjuvant and obtained a protection percentage of 73.2% measured by reducing the oocysts shedding number after challenge. This difference in the reduction between these studies might be due to the different adjuvants used in each study; however, further studies need to be done to corroborate this theory.…”

Section: Discussionmentioning

confidence: 99%

“…The histopathological evaluation was done as described (GARCIA et al, 2008). Briefly, intestinal sections of the birds were collected at necropsy, fixed in 10% buffered formalin solution, and routinely processed for histopathological evaluation.…”

Section: Oocyst Count Lesion Score and Histopathological Evaluationmentioning

confidence: 99%

“…The ELISA assay was done as described (GARCIA et al, 2008). The dilutions were established by using comparisons of the dilutions exiting between the serum, antigens, and conjugate.…”

Section: Immunoenzymatic Assay (Elisa)mentioning

confidence: 99%

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The immunogenicity of Eimeria tenella sporozoite proteins and living oocyst vaccines in broilers

Bogado¹,

Garcia²,

Headley³

et al. 2012

Sem. Ci. Agr.

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The purpose of the present study was to evaluate the immunity of broilers inoculated with sporulated oocysts and sporozoite proteins of Eimeria tenella against challenge with homologous infectives oocysts. Broiler chickens of Hubbard strain, females, coccidian-free, were kept in wire cages and inoculated on the third day (day 0). Three treatments were used: T1, the negative control; T2, received 500 sporulated oocysts by gavage; T3, positive control; T4, received 50 μg of sporozoite protein with Quil A; and T5, received Quil A without sporozoite protein and PBS, the last two protocols were administered via nasal route on days 0, 7, and 21. On the 31st day, all groups, with the exception of T1, were challenged with homologous virulent strain of E. tenella at the dose of 8.0×10 4 oocysts. Immunogenicity was assessed by carotenoids, ELISA assay, histopathological analysis, oocysts count and lesion score. Antibody kinetics were measured weekly and showed a gradual increase in levels of immunoglobulin for treatment T2 and T4, reaching a peak on day 21. Based on the parameters evaluated, a total protecting immunological effect for the infectant oocysts vaccine (T2) and partial protection for the sporozoite protein vaccine (T4) against homologous virulent strain challenge were observed.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Oocyst Count Lesion Score and Histopathological Evaluationmentioning

confidence: 99%

“…The ELISA assay was done as described (GARCIA et al, 2008). The dilutions were established by using comparisons of the dilutions exiting between the serum, antigens, and conjugate.…”

Section: Immunoenzymatic Assay (Elisa)mentioning

confidence: 99%

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The immunogenicity of Eimeria tenella sporozoite proteins and living oocyst vaccines in broilers

Bogado¹,

Garcia²,

Headley³

et al. 2012

Sem. Ci. Agr.

Self Cite

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“…These results suggest that this route of vaccination could also be effective in inducing protection against avian coccidiosis (Garcia et al 2008).…”

Section: Methods Of Vaccinationmentioning

confidence: 78%

Control of poultry coccidiosis: changing trends

Tewari

Maharana

2011

J Parasit Dis

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Coccidiosis is the most important protozoan disease affecting the poultry industry worldwide. Control of poultry coccidiosis is presently based on managerial skills and the use of prophylactic coccidiostatic drugs. With the emergence of drug resistant Eimeria strains, emphasis has been laid on development and use of safer vaccines; some of them have been commercialized successfully. The present review deals with the various factors responsible for the development of clinical coccidiosis in poultry as well as an overview of the currently available inducers and boosters of immunity against coccidiosis. There are three groups of vaccines currently available against coccidiosis which can be distinguished on the basis of characteristics of the Eimeria species included in the respective products, viz. vaccines based on live virulent strains, vaccines based on live attenuated strains, and vaccines based on live strains that are relatively tolerant to the ionophore compounds. The latter vaccine combines the early chemotherapeutic effect of ionophores with the late prophylactic effect of vaccination. Although in the near future more varieties of oocyst based live vaccines are expected, identification of selective coccidian-specific immunoprotective molecules is likely to get more attention to facilitate the sustainable control of poultry coccidiosis.

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Prokaryotic expression and identification of 3-1E gene of merozoite surface antigen of Eimeria acervulina

Zhao

Wang

et al. 2011

Parasitol Res

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Eimeria acervulina was isolated from chicken at Hebei province, China. The gene of merozoite surface antigen 3-1E was amplified and cloned into pET28a(+) vector and then transformed into Escherichia coli BL21 strain. Results showed that 3-1E fusion protein band of about 22 kDa was identified by SDS-PAGE. Western blot analysis indicated that the recombinant protein specifically reacted with E. acervulina polyclonal antibody.

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Eimeria tenella: Utilization of a nasal vaccine with sporozoite antigens incorporated into Iscom as protection for broiler breeders against a homologous challenge

Cited by 14 publications

References 32 publications

The immunogenicity of Eimeria tenella sporozoite proteins and living oocyst vaccines in broilers

The immunogenicity of Eimeria tenella sporozoite proteins and living oocyst vaccines in broilers

Control of poultry coccidiosis: changing trends

Prokaryotic expression and identification of 3-1E gene of merozoite surface antigen of Eimeria acervulina

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