Eimeria tenella: Interleukin 17 contributes to host immunopathology in the gut during experimental infection

Zhang, Lei; Liu, Renqiang; Song, Meng; Hu, Yanxin; Pan, Baoliang; Cai, Jianping; Wang, Ming

doi:10.1016/j.exppara.2012.11.009

Cited by 60 publications

(63 citation statements)

References 38 publications

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“…Our previous studies have demonstrated that chIL-17A and chIL-17F of CEF induced the expression of inflammatory cytokines such as IL-1␤, IL-6, or IL-8 (19,22,23). We have described here full-length cDNA encoding chicken IL-17RA and demonstrated specific siRNA-mediated inhibition of inflammatory cytokine transcripts in CEF cells stimulated with chIL-17A or chIL-17F.…”

Section: Discussionmentioning

confidence: 99%

“…The chIL-17A expression increases in the duodenum and jejunum of E. acervulina-and E. maximainfected chickens, respectively (53,54). Expression levels of chIL-17A transcript are upregulated or downregulated in the cecum of E. tenella-infected chickens (19,22,53). In the present study, when chickens were infected with E. tenella (cecum) or E. maxima (jejunum), the chIL-17A expression increased during E. maxima infection but was unchanged during E. tenella infection.…”

Section: Discussionmentioning

confidence: 99%

“…The growing literature on the importance of the IL-17/IL-17R axis in host defense includes many studies that describe the critical role of the IL-17 family of cytokines in avian immune responses (19)(20)(21)(22). Three cytokines-IL-17A, IL-17D, and IL-17F-have been identified in an expressed sequence tag (EST) cDNA library prepared from intestinal intraepithelial lymphocytes (IELs) of Eimeria-infected chickens (23), from a testis cDNA library prepared from Korean native chickens (24), and from concanavalin A (ConA)-activated chicken splenic lymphocytes (19), respectively.…”

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confidence: 99%

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Downregulation of Chicken Interleukin-17 Receptor A during Eimeria Infection

et al. 2014

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e Both interleukin-17A (IL-17A) and IL-17F are proinflammatory cytokines that have an important role in intestinal homeostasis via receptor signaling. These cytokines have been characterized in chickens, but very little is known about their receptors and their functional activity. We provide here the first description of the sequence analysis, bioactivity, and comparative expression analysis of chicken IL-17RA (chIL-17RA) in chickens infected with Salmonella and Eimeria, two major infectious agents of gastrointestinal diseases of poultry of economic importance. A full-length chIL-17RA cDNA with a 2,568-bp coding region was identified from chicken thymus cDNA. chIL-17RA shares ca. 46% identity with mammalian homologues and 29.2 to 31.5% identity with its piscine counterparts. chIL-17RA transcript expression was relatively high in the thymus and in the chicken macrophage cell line HD11. The chIL-17RA-specific small interfering RNA inhibits interleukin-6 (IL-6), IL-8, and IL-1␤ mRNA expression in chicken embryo fibroblast cells (but not in DF-1 cells) stimulated with chIL-17A or chIL-17F. Interaction between chIL-17RA and chIL-17A was confirmed by coimmunoprecipitation. Downregulation of chIL-17RA occurred in concanavalin A-or lipopolysaccharide-activated splenic lymphocytes but not in poly(I·C)-activated splenic lymphocytes. In Salmonella-and Eimeria-infected chickens, the expression levels of the chIL-17RA transcript were downregulated in intestinal tissues from chickens infected with two Eimeria species, E. tenella or E. maxima, that preferentially infect the cecum and jejunum, respectively. However, chIL-17RA expression was generally unchanged in Salmonella infection. These results suggest that chIL-17RA has an important role in mucosal immunity to intestinal intracellular parasite infections such as Eimeria infection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Downregulation of Chicken Interleukin-17 Receptor A during Eimeria Infection

et al. 2014

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“…Several infection studies with various protozoal species demonstrated that Th17 cells may mediate the host defense against Trypanosoma cruzi [27], Pneumocystis carinii [28] and Toxoplasma gondii [29]. However, controversial results have been shown in human cutaneous leishmaniasis [30], T. gondii infection [31] and Eimeria tenella infection in chicken [32], which suggests that IL-17 contributes to the pathology of these infections.…”

Section: Introductionmentioning

confidence: 99%

The expression dynamics of IL-17 and Th17 response relative cytokines in the trachea and spleen of chickens after infection with Cryptosporidium baileyi

et al. 2014

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BackgroundCryptosporidium baileyi is the dominant Cryptosporidium species in birds causing emerging health problems in the poultry industry, and is also a model to study the biology of Cryptosporidium spp.. IL-17 (also called IL-17A) is a hallmark pro-inflammatory cytokine of Th17 cells that plays an important role in several human autoimmune diseases and microbial infection disease of many animals, and it may play a role in Cryptosporidium infection.MethodsThe present study examined the mRNA level of IL-17 and Th17 response relative cytokines in the trachea and spleen of C. baileyi-infected chickens at different time points using real-time quantitative PCR (qPCR).ResultsAll examined cytokines in the trachea were up-regulated in the infected chickens compared with the uninfected control during C. baileyi infection. Significant increased IL-17 mRNA level in the trachea was observed as early as 12 h post infection (pi), peaking at 24 h pi and 10 d pi, and declining thereafter. The transcription levels of IL-17 and Th17 response relative cytokines in spleen were also significantly increased at different time points during the infection.ConclusionsIL-17 was indicated to participate in the induction of inflammation during infection of some intracellular protozoan parasites. The results in the present study suggest that IL-17 may play a role in immunity against Cryptosporidium infection, and provide basic information for determining the role of Th17 cell in Cryptosporidium infection.

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“…However, these results seem to be controversial to previous studies. Th17-related cytokines displayed significantly higher level during oral experimental E. tenella infection, which indicated that IL-17 might facilitate the pathogenicity of E. tenella [32]. The expression of TGF-β mRNA was significantly increased in both the spleen and intestine following E. acervulina infection [33].…”

Section: Discussionmentioning

confidence: 96%