Eimeria genomics: Where are we now and where are we going?

Blake, Damer P.

doi:10.1016/j.vetpar.2015.05.007

Cited by 47 publications

(36 citation statements)

References 81 publications

(104 reference statements)

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“…Expanding knowledge of the phylogenetic evolutionary relatedness among Eimeria species can provide invaluable insights into coccidian biology, immunology and metabolic requirements, as well as underpin improvement in the development of anticoccidial control using existing and novel drugs or even vaccines (Blake, 2015). Therefore, we call for more sequences from clearly neglected Eimeria species of caprine species to be produced and available on GenBank in order to develop a more robust molecular taxonomy.…”

Section: Discussionmentioning

confidence: 99%

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A newly described strain of Eimeria arloingi (strain A) belongs to the phylogenetic group of ruminant-infecting pathogenic species, which replicate in host endothelial cells in vivo

Silva

Chávez-Maya

Macdonald

et al. 2017

Veterinary Parasitology

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Section: Discussionmentioning

confidence: 99%

“…Currently, more than 1200 Eimeria species are known (Chapman et al, 2013) and it is assumed that many more remain to be discovered (Blake, 2015). The great majority of these species are monoxenous enteropathogens of vertebrates which usually induce only mild pathology and mild or non-clinical disease.…”

Section: Introductionmentioning

confidence: 99%

A newly described strain of Eimeria arloingi (strain A) belongs to the phylogenetic group of ruminant-infecting pathogenic species, which replicate in host endothelial cells in vivo

Silva

Chávez-Maya

Macdonald

et al. 2017

Veterinary Parasitology

Self Cite

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“…The severity of disease is influenced by multiple factors including parasite and host genotype, poultry management system, previous exposure history and especially oocyst dose (Blake 2015). According to Conway and McKenzie (2007), a dose of 10 4 to 10 5 sporulated E. tenella oocysts is required to induce a severe clinical response in broilers.…”

Section: Discussionmentioning

confidence: 99%

Effects of subclinical Eimeria tenella infection on Pectoralis major muscle in broiler chickens

Chodová

Tůmová

Sládková

et al. 2017

Italian Journal of Animal Science

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The aim of this study was to evaluate changes in the histological characteristics of the pectoralis major (PM) muscle in chickens as well as histological changes in the caecum after a low dose of Eimeria tenella (E. tenella). The chickens were inoculated orally with 5 Â 10 2 E. tenella oocysts (Gr1) and 1 Â 10 3 E. tenella oocysts (Gr2) at 16 days of age. Six chickens from each group were sacrificed for post mortem examination at 9 days post-infection (pi) and 16 days pi. Chicken growth was not affected by infection. On the 9th day of infection, caecal villus height was significantly greater in the non-infected control group. However, infected chickens from group Gr1 sacrificed seven days later had an even greater caecal villus height than those of the control (p ¼.001). Both infected groups had higher PM weights at 16 days pi than did the control group (p ¼ .001). The fibre cross-sectional area was smaller in Gr2 at 9 days pi; however, this parameter was larger in these chickens at 16 days pi. The pH value of PM of group Gr2 was significantly higher than that of the control group or group Gr1 at 16 days pi (p ¼ .020). ARTICLE HISTORY

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“…In recent years, finding key molecules in the host-parasite interaction is becoming an alternative approach for analyzing pathogenic mechanisms and exploring novel targets for developing drugs and vaccine candidates to control parasitic diseases [16][17][18][19][20]. Using the complementary DNA (cDNA) microarray technique, a great number of differentially regulated mRNA molecules in chicken intestines have been identified after infections with E. tenella [4,21], E. acervulina [21][22][23][24] and E. maxima [9,21,22].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of differentially expressed profiles of mRNAs, lncRNAs and circRNAs in chickens during Eimeria necatrix infection

et al. 2020

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Background: Eimeria necatrix, the most highly pathogenic coccidian in chicken small intestines, can cause high morbidity and mortality in susceptible birds and devastating economic losses in poultry production, but the underlying molecular mechanisms in interaction between chicken and E. necatrix are not entirely revealed. Accumulating evidence shows that the long-non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) are key regulators in various infectious diseases. However, the expression profiles and roles of these two non-coding RNAs (ncRNAs) during E. necatrix infection are still unclear. Methods: The expression profiles of mRNAs, lncRNAs and circRNAs in mid-segments of chicken small intestines at 108 h post-infection (pi) with E. necatrix were analyzed by using the RNA-seq technique. Results: After strict filtering of raw data, we putatively identified 49,183 mRNAs, 818 lncRNAs and 4153 circRNAs. The obtained lncRNAs were classified into four types, including 228 (27.87%) intergenic, 67 (8.19%) intronic, 166 (20.29%) anti-sense and 357 (43.64%) sense-overlapping lncRNAs; of these, 571 were found to be novel. Five types were also predicted for putative circRNAs, including 180 exonic, 54 intronic, 113 antisense, 109 intergenic and 3697 senseoverlapping circRNAs. Eimeria necatrix infection significantly altered the expression of 1543 mRNAs (707 upregulated and 836 downregulated), 95 lncRNAs (49 upregulated and 46 downregulated) and 13 circRNAs (9 upregulated and 4 downregulated). Target predictions revealed that 38 aberrantly expressed lncRNAs would cis-regulate 73 mRNAs, and 1453 mRNAs could be trans-regulated by 87 differentially regulated lncRNAs. Additionally, 109 potential sponging miRNAs were also identified for 9 circRNAs. GO and KEGG enrichment analysis of target mRNAs for lncRNAs, and sponging miRNA targets and source genes for circRNAs identified associations of both lncRNAs and circRNAs with host immune defense and pathogenesis during E. necatrix infection. Conclusions: To the best of our knowledge, the present study provides the first genome-wide analysis of mRNAs, lncRNAs and circRNAs in chicken small intestines infected with E. necatrix. The obtained data will offer novel clues for exploring the interaction mechanisms between chickens and Eimeria spp.

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Eimeria genomics: Where are we now and where are we going?

Cited by 47 publications

References 81 publications

A newly described strain of Eimeria arloingi (strain A) belongs to the phylogenetic group of ruminant-infecting pathogenic species, which replicate in host endothelial cells in vivo

A newly described strain of Eimeria arloingi (strain A) belongs to the phylogenetic group of ruminant-infecting pathogenic species, which replicate in host endothelial cells in vivo

Effects of subclinical Eimeria tenella infection on Pectoralis major muscle in broiler chickens

Genome-wide analysis of differentially expressed profiles of mRNAs, lncRNAs and circRNAs in chickens during Eimeria necatrix infection

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