Eimeria bovis: An update on parasite–host cell interactions

Hermosilla, Carlos; Ruiz, Antonio; Taubert, Anja

doi:10.1016/j.ijmm.2012.07.002

Cited by 41 publications

(34 citation statements)

References 58 publications

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“…In vitro accessibility and mass production of E. bovis meronts II is of particular scientific interest for numerous reasons since (i) it allows for studies on merozoite II-host cell interactions which, as demonstrated for E. bovis sporozoite infections, are likely to be multifactorial and to rely on several regulatory processes to guarantee parasite replication Lang et al 2009;Taubert et al 2010;Ruiz et al 2010;Lutz et al 2011;Hermosilla et al 2012);(ii) there is some evidence that meronts II represent the major targets for the protective immune response in animals immune to Eimeria spp. infections (Rose et al 1992a, b;Shi et al 2001a, b;Taubert et al 2008;Hermosilla et al 2014); (iii) these cultures are useful for the screening of pharmaceutical, immunological, or biochemical compounds against Eimeria (Hamid et al 2014;Silva et al 2015); and (iv) it offers the possibility to perform stage-specific analyses at the proteomic or transcriptomic level as demonstrated for E. bovis sporozoiteinfected host cells Lutz et al 2011).…”

Section: Gm-csfmentioning

confidence: 99%

“…Furthermore, GM-CSF also was found upregulated in T. gondii- (Channon et al 2002;Taubert et al 2006a, b), N. caninum-, and E. bovis-infected host cells (Taubert et al 2006a, b). GM-CSF and IL-6 both have an impact on macrophages, dendritic cells, monocytes, and T cells and therefore represent key molecules for the transition from innate into adaptive immunity (Taubert et al 2006a(Taubert et al , b, 2010Hermosilla et al 2012).…”

Section: Gm-csfmentioning

confidence: 99%

“…The characteristics of the initial immune responses can greatly impact the resulting clinical outcome. As such, in vitro infections with E. bovis sporozoites significantly altered immune-associated reactions of host cells as shown by the regulation of a molecular network involving various chemokines (CXCL8, CXCL1l, CXCL3, CXCL6), i.e., of molecules acting predominately on granulocytes and/or T lymphocytes (Zlotnik and Yoshie 2000;Zlotnik et al 2006), both of which are stimulated in E. bovis infections Hermosilla et al 2012). The here detected profiles of immunomodulatory molecules strongly suggest subsequent cellular migration of several immune cell types in parasitized mucosal tissue, which may partially be reflected in reports on macrophage ) and T cell (Sühwold et al 2010) infiltration into the gut of E. bovis-infected calves.…”

Section: Gm-csfmentioning

confidence: 99%

“…Taking into account that Eimeria infections are highly hostand host cell-specific and that stage-specific innate immune reactions are a common feature Muñoz-Caro et al 2015), it appears essential that basic research is performed on different developmental stages in the respective host cell type (Hermosilla et al 2012). Nonetheless, fully permissive in vitro systems, representing one important prerequisite for any basic investigation related to specific parasite stages-host cell interactions, allowing for the production of any stage of question are still lacking for E. bovis merozoites I/II or gamonts while they have been successfully established for chicken Eimeria species, e.g., Eimeria tenella (Hofmann and Raether 1990).…”

Section: Introductionmentioning

confidence: 99%

“…Nonetheless, fully permissive in vitro systems, representing one important prerequisite for any basic investigation related to specific parasite stages-host cell interactions, allowing for the production of any stage of question are still lacking for E. bovis merozoites I/II or gamonts while they have been successfully established for chicken Eimeria species, e.g., Eimeria tenella (Hofmann and Raether 1990). Well-established in vitro systems for E. bovis (Hammond and Fayer 1968;Speer et al 1985;Speer 1986, 1987;Hermosilla et al 2002;Taubert et al 2006a;Behrendt et al 2004Behrendt et al , 2008 use cell types which differ from the specific host cell of merozoites I in vivo and may therefore only partially mirror the spectrum of early innate reactions of the adequate host cell (Hermosilla et al 2006;Taubert et al 2006a, b;Hermosilla et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Suitable in vitro culture of Eimeria bovis meront II stages in bovine colonic epithelial cells and parasite-induced upregulation of CXCL10 and GM-CSF gene transcription

et al. 2015

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We here established a suitable in vitro cell culture system based on bovine colonic epithelial cells (BCEC) for the development of Eimeria bovis merozoites I and the characterization of early parasite-induced innate epithelial host cell reactions as gene transcription of proinflammatory molecules. Both primary and permanent BCEC (BCEC (rim) and BCEC(perm)) were suitable for E. bovis merozoite I invasion and subsequent development of meronts II leading to the release of viable merozoites II. E. bovis merozoite II failed to develop any further neither into gamont nor oocyst stages in BCEC in vitro. E. bovis merozoite I induced innate epithelial host cell reactions at the level of CXC/CCL chemokines (CXCL1, CXCL8, CXCL10, CCL2), IL-6, and GM-CSF gene transcription. Overall, both BCEC types were activated by merozoite I infections since they showed significantly enhanced gene transcript levels of the immunomodulatory molecules CXCL10 and GM-CSF. However, gene transcription profiles of BCEC(prim) and BCEC(perm) revealed different reaction patterns in response to merozoite I infection with regard to quality and kinetics of chemokine/cytokine gene transcription. Although both BCEC types equally showed most prominent responses for CXCL10 and GM-CSF, the induction of CXCL1, CXCL8, CCL2, and IL-6 gene transcripts varied qualitatively and quantitatively. Our results demonstrate that BCEC seem capable to respond to E. bovis merozoite I infection by the upregulation of CXCL10 and GM-CSF gene transcription and therefore probably contribute to host innate effector mechanisms against E. bovis.

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Section: Gm-csfmentioning

confidence: 99%

Section: Gm-csfmentioning

confidence: 99%

Section: Gm-csfmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Suitable in vitro culture of Eimeria bovis meront II stages in bovine colonic epithelial cells and parasite-induced upregulation of CXCL10 and GM-CSF gene transcription

et al. 2015

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Atmospheric‐pressure scanning microprobe matrix‐assisted laser desorption/ionization mass spectrometry imaging of Neospora caninum‐infected cell monolayers

Anschütz

Gerbig

Ventura

et al. 2022

Analytical Science Advances

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Neospora caninum is an obligate intracellular protozoan parasite of the phylum Alveolata (subphylum Apicomplexa) which has not been studied extensively in a biochemical context. N. caninum is a primary cause of reproductive disorders causing mummification and abortion not only in cattle but also in other small ruminant species resulting in a substantial economic impact on the livestock industry. In canids, which are the final hosts of N. caninum, clinical disease includes neuromuscular symptoms, ataxia, and ascending paralysis. Fatal outcomes of neosporosis have also been reported depending on the host species, age and immune status, however, its zoonotic potential is still uncertain. Therefore, N. caninum should be thoroughly investigated. Matrix‐assisted laser desorption/ionisation (MALDI) mass spectrometry (MS) and MS imaging (MSI) were used, combined with high‐performance liquid chromatography (HPLC) to investigate these intracellular parasites. The aim of this study was to identify molecular biomarkers for N. caninum tachyzoite‐infected host cells and to further clarify their functions. By atmospheric‐pressure scanning microprobe MALDI MS(I), sections of N. caninum‐infected and non‐infected host cell pellets were examined in order to determine potential markers. In vivo, N. caninum infects different types of nucleated cells, such as endothelial cells which represent a highly immunoreactive cell type. Therefore, primary bovine umbilical vein endothelial cells were here used as a suitable infection system. For comparison, the permanent MARC‐145 cell line was used as an additional, simplified in vitro cell culture model. HPLC‐tandem MS (HPLC‐MS/MS) experiments combined with database search were employed for structural verification of markers. The statistically relevant biomarkers found by MS and identified by HPLC‐MS/MS measurements were partly also found in infected monolayers. Marker signals were imaged in cell layers of N. caninum‐infected and non‐infected host cells at 5 µm lateral resolution.

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Effect of biosynthesized nanoselenium using Azadirachta indica (Meliaceae) leaf extracts against Eimeria papillata infection

Abdel‐Gaber

Hawsah

Al-Shaebi

et al. 2023

Microscopy Res & Technique

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Coccidiosis is a protozoan parasitic disease affecting different animal species. Resistance has been reported for all available anticoccidial drugs. Recently, green synthesis of nanoparticles is considered a new therapeutic tool against this parasitic disease. The present work aimed to study the effect of biosynthesized nanoselenium from Azadirachta indica leaf extracts (BNS) against Eimeria papillata‐induced infection in mice. The phytochemical analysis of leaf extracts contained 33 phytochemical components. The BNS was spherical with ⁓68.12 nm in diameter and an absorption peak at 308 nm via UV‐spectra. The data showed that mice infected with E. papillata revealed the highest oocyst output on the 5th‐day post‐infection (p.i.). Infection also induced injury and inflammation of the mice jejunum. Treatment with BNS resulted in a 97.21% suppression for the oocyst output. The treated groups with BNS showed enhancement in feed intake as compared to the infected group. Histological examinations showed a significant reduction in the intracellular developmental Eimeria stages in the jejunal tissues of infected‐treated mice of about 24.86 ± 2.38 stages/10 villous crypt units. Moreover, there was a significant change in the morphometry for Eimeria stages after the treatment with BNS. Infection induced a disturbance in the level of carbohydrates and protein contents in the infected mice which enhanced after treatment with BNS. In addition, BNS counteracted the E. papillata‐induced loss of the total antioxidant capacity. Collectively, BNS is considered a promising anticoccidial and antioxidant effector and could be used for the treatment of coccidiosis.

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Eimeria bovis: An update on parasite–host cell interactions

Cited by 41 publications

References 58 publications

Suitable in vitro culture of Eimeria bovis meront II stages in bovine colonic epithelial cells and parasite-induced upregulation of CXCL10 and GM-CSF gene transcription

Suitable in vitro culture of Eimeria bovis meront II stages in bovine colonic epithelial cells and parasite-induced upregulation of CXCL10 and GM-CSF gene transcription

Atmospheric‐pressure scanning microprobe matrix‐assisted laser desorption/ionization mass spectrometry imaging of Neospora caninum‐infected cell monolayers

Effect of biosynthesized nanoselenium using Azadirachta indica (Meliaceae) leaf extracts against Eimeria papillata infection

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