Eikenella corrodens adherence to human buccal epithelial cells

Abstract

The mechanism of Eikenella corrodens adherence to human buccal epithelial cells in vitro was studied. Initial experiments to determine the optimal conditions for adherence of E. corrodens to buccal epithelial cells showed that adherence was dependent on time, temperature, bacterial concentration, and pH. Different strains of E. corrodens varied in their ability to adhere, and strain 1073 showed the greatest ability in adherence. Strain 1073 was selected for studies of adherence mechanisms. Trypsin treatment or heating (1000C, 10 min) of the bacterial cells abolished their capacity to adhere to buccal epithelial cells. Treatment of buccal epithelial cells with trypsin also abolished adherence of E. corrodens 1073, whereas neuraminidase treatment of buccal epithelial cells enhanced the adherence. The adherence was inhibited by ethylenediaminetetraacetic acid and restored by adding Ca2". The adherence was remarkably inhibited by sugars containing D-galactose and N-acetyl-D-galactosamine. Treatment of neuraminidase-treated epithelial cells with sodium metaperiodate or a-and ,-galactosidase did not decrease the adherence. These data suggest that adherence of E. corrodens 1073 to human buccal epithelisl cells may require the interaction of lectinlike proteins on the bacterial surface with galactose-like receptors on the surface of epithelial celLs.

Correlation between cell‐adherent activity and surface structure in Porphyromonas gingivalis

“…First, the adherent ability of P. gingivalis to cultured human fibroblasts such as Gin I cells and human periodontai ligament fibroblasts and epithelial cells (Ca9-22) was examined. Many investi- gators have reported bacterial attachment to buccal cells collected by scraping the oral mucosal surface (23,26,34,45). In this experiment, we used cultured cells originating from human buccal regions as the target cells in order to prevent the experimental error caused by the variation in volunteer cells.…”

Production and characterization of monoclonal antibodies against bacterial lectin of Eikenella corrodens

“…Reactivity of antibodies to E. corrodens 1073 and E. corrodens 1 080 tination activity than E. corrodens 1073 cells did (13,15). In addition, coaggregation activity of E. corrodens 1080 with A. vis casus or S. sanguis was weaker than that of E. corrodens 1073 (data not shown).…”

“…Monoinfection of germfree, or gnotobiotic rats with E. corrodens 1073 has been re-ported to cause periodontal disease with sever alveolar bone loss (10)(11)(12). Previously, we found that E. corrodens I 073 had a cell-associated lectinlike substance (EeLS) and it functioned in the adherence of this oral bacterium to various cell surface (13)(14)(15). We also found that EeLS mediated N-acetyl-D-galactosamine (GalNAc)-inhibitable coaggregation of E. corrodens with certain strains of Streptococcus sanguis and Actinomyces l'iscosus in vitro ( 16) which were prominent in the earliest stages of plaque formation (8,17).…”

Carbohydrates as recognition molecules in infection and immunity

“…Corynebacterium parvum has also been shown to bind to phagocytes by a mannose-specific lectin binding site in the cell wall of the bacterium [20]. Other microorganisms that rely on lectin type adhesion are Klebsiella pneumonia, various species of Salmonella, Shigella and Proteus [8, 21,22], Eikenella corrodens, Aeromonos hydrophilia and Fusobacterium nucleatum [23][24][25].…”