Eighteen cases of liver injury following ingestion of Polygonum multiflorum

Dong, Hai‐Lin; Slain, Douglas; Cheng, Jie; Ma, Weirui; Liang, Weifeng

doi:10.1016/j.ctim.2013.12.008

Cited by 96 publications

(81 citation statements)

References 17 publications

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“…4C, D). This was similar to previous reports, 29,30) which showed that liver injury occurred following the ingestion of P. multiflorum. This suggested that P. multiflorum extract exerts a modest toxic effect in the tested dosage.…”

supporting

confidence: 93%

“…SH-SY5Y Cells were grown at 37°C in an incubator with 5% CO 2 . To investigate the effects of P. multiflorum and S. commixta extracts on cell viability, cells were pre-treated with P. multiflorum or S. commixta extract for 30 min, and then treated with 25 µM Aβ [25][26][27][28][29][30][31][32][33][34][35] (Sigma-Aldrich) for a further 24 h. Then, the cells were incubated with 2 mg/mL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma-Aldrich) at 37°C in a CO 2 incubator for 3 h. MTT medium was carefully aspirated, and the formazan dye was eluted using dimethyl sulfoxide (DMSO). The plate was shaken, and the absorbance was read using a spectrophotometer (Versamax) at a wavelength of 580 nm.…”

Section: Fly Stocks and Maintenancementioning

confidence: 99%

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In Vivo Screening of Traditional Medicinal Plants for Neuroprotective Activity against Aβ42 Cytotoxicity by Using Drosophila Models of Alzheimer’s Disease

Liu

Lee

Kim

et al. 2015

Biological & Pharmaceutical Bulletin

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Alzheimer's disease (AD) is the most common neurodegenerative disorder, characterized by progressive neuronal loss with amyloid β-peptide (Aβ) plaques. Despite several drugs currently used to treat AD, their beneficial effects on AD progress remains under debate. Here, we established a rapid in vivo screening system using Drosophila AD models to assess the neuroprotective activities of medicinal plants that have been used in traditional Chinese medicine. Among 23 medicinal plants tested, the extracts from five plants, Coriandrum sativum, Nardostachys jatamansi, Polygonum multiflorum (P. multiflorum), Rehmannia glutinosa, and Sorbus commixta (S. commixta), showed protective effects against the Aβ42 neurotoxicity. We further characterized the neuroprotective activity of ethanol extracts from P. multiflorum and S. commixta. Aβ42-expressing flies that we used showed AD neurological phenotypes, such as decreased survival and motility and increased cell death and reactive oxygen species level. However, feeding these flies extracts from P. multiflorum or S. commixta showed strong suppression of such phenotypes. Similar results were observed in human cells, so that the treatment of P. multiflorum and S. commixta extracts increased the viability of Aβ-treated SH-SY5Y cells. Moreover, 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside, one of the main constituents of P. multiflorum, also showed similar protective activity against Aβ42 cytotoxicity in both Drosophila and human cells. Taken together, our results suggest that both P. multiflorum and S. commixta have therapeutic potential for the treatment of neurodegenerative diseases, such as AD.Key words amyloid β-peptide 42 (Aβ42); Alzheimer's disease; Drosophila; Polygonum multiflorum; reactive oxygen species (ROS); Sorbus commixta Alzheimer's disease (AD) is a common neurodegenerative disease that is characterized by progressive neural loss caused by amyloid β-peptide (Aβ) plaques and cognitive deficits. 1,2) During disease progression, neurotoxic forms of Aβ cause neuronal damages via various cellular abnormalities such as increased oxidative damage, impaired energy metabolism, disrupted cellular calcium homeostasis, and increased inflammatory response.2,3) Several drugs have been approved to treat AD, given the dysfunction of cholinergic and glutamatergic neurotransmission; they function as acetylcholinesterase inhibitors or N-methyl-D-aspartate (NMDA) receptor antagonists. 4,5) However, the beneficial effects of these drugs during the progression of AD are still in question. 6) Due to its complex etiology, a combination therapy including the use of medicinal plants with effective components has been proposed as an alternative choice for treatment of AD. 7,8) Chinese traditional medicine has employed a variety of herbs to treat dementia, and a number of neuroprotective agents were isolated from these herbs. 7,8) For example, Polygonum multiflorum (P. multiflorum; syn. Reynoutria multiflora) has been known as a tonic and antiaging agent in many remedies for centuries, and...

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supporting

confidence: 93%

Section: Fly Stocks and Maintenancementioning

confidence: 99%

In Vivo Screening of Traditional Medicinal Plants for Neuroprotective Activity against Aβ42 Cytotoxicity by Using Drosophila Models of Alzheimer’s Disease

Liu

Lee

Kim

et al. 2015

Biological & Pharmaceutical Bulletin

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“…In addition, an adverse hepatic reaction of RPM and its preparations have been reported. 18,19) However, its toxicity and efficacy were studied separately, and again lack systematic research. Therefore, it is necessary to now investigate both the lipid-lowering effect and hepatotoxicity of RPM extract simultaneously.…”

Section: )mentioning

confidence: 99%

“…However, the adverse hepatic reaction of RPM and its preparations have increasingly been reported. 18,19) Thus, we investigated the hepatotoxicity of RPM extract comprehensively in this study. Effects of RPM Extract on Plasma Biochemical Indexes ALP, TBA, ALT and AST were commonly used to evaluate liver function.…”

Section: Study On Liver Toxicitymentioning

confidence: 99%

The Anti-hyperlipidemia Effects of Raw Polygonum multiflorum Extract in Vivo

Xian

Liu

et al. 2017

Biological & Pharmaceutical Bulletin

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Polygonum multiflorum is widely used in the prevention and treatment of hyperlipidemia in traditional Chinese Medicine. In this study, the effects and relevant mechanisms of lipid-regulation by raw Polygonum multiflorum (RPM) were investigated. The results indicated that the basal plasma lipids, such as low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), and triglycerides (TG), were significantly decreased in RPM treatment groups compared with the model group, especially in the RPM high dose group. The key enzymes involved in lipid metabolism, 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) in plasma were generally reduced after oral administration, which was consistent with the transcription levels of their target genes. In addition, the hepatotoxicity of RPM was investigated, and RPM showed slightly less liver injury than that induced by simvastatin. Histological analysis indicated that the fat vacuoles and steatosis in hepatocytes were relieved after oral administration of RPM extract at a high dose of 16.2 g/kg, which was more obvious than that induced by simvastatin. These results revealed that RPM exerted its lipid-lowering effect by regulating the expression of related genes, and performed better than simvastatin in the treatment of hyperlipidemia. Key words raw Polygonum multiflorum; hyperlipidemia; lipid-lowering; hepatotoxicityHyperlipidemia is a common metabolic syndrome characterized by increased total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) and decreased high-density lipoprotein cholesterol (HDL-C) levels, 1) which facilitate endothelial dysfunction and atherogenesis, the major risk factor for cardiovascular disease.2) Statins are commonly used in clinical treatment as first-line lipid-lowering drugs, especially in the treatment of hyperlipidemia. They act to suppress the activities of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) and thus affect the formation of mevalonic acid, the rate-limiting step in the biosynthesis of cholesterol, 3) but this treatment is often accompanied by adverse reactions such as gastrointestinal tract issues, myopathy and hepatotoxicity. 4,5) Traditional Chinese Medicine (TCM) is a healthcare-focused medical system based on more than 3000 years of continuous practice experience, and therefore has its own advantages and characteristics in early intervention and personalized treatment. Some Chinese medicinal herbs have shown lipid-lowering effects by reducing the absorption of exogenous lipids and the synthesis of endogenous lipids, and also by facilitating the transportation, metabolism and excretion of lipids. 6)TCM performs multi-target network regulation based on the function of the whole body; between its characteristic good efficiency and low toxicity, TCM has received extensive attention all over the world. There are various Chinese medicinal herbs clinically prescribed to cure hyperlipidemia. Among these herbs, Polygonum multiflorum ranked as...

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“…The Medicines and Healthcare Products Regulatory Agency (MHRA) also released relevant information regarding adverse reactions to PM. Liver injury, especially acute liver injury, due to PM consumption has recently been reported worldwide [2-4]. …”

Section: Introductionmentioning

confidence: 99%

Application of iTRAQ-Based Quantitative Proteomics Approach to Identify Deregulated Proteins Associated with Liver Toxicity Induced by Polygonum Multiflorum in Rats

Lin

et al. 2017

Cell Physiol Biochem

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Background/Aims: Clinical reports on adverse reactions that result from Polygonum multiflorum (PM) and its preparations, especially regarding liver injury, have recently received widespread attention. This study aimed to investigate the mechanism of hepatotoxicity induced by different PM extracts through iTRAQ quantitative proteomics. Methods: The different PM extracts were orally administrated for 90 days to rats, and the hepatotoxicity effect was evaluated through measurement of biochemical indexes, oxidative damage indexes and hematoxylin-eosin (HE) staining. Then, the hepatotoxicity mechanism was investigated by iTRAQ quantitative proteomics. Results: The results of biochemical and histopathological analyses showed that liver injury occurred in all groups of rats given by various PM extracts, which proved all of the PM extracts could induce hepatotoxicity. The hepatotoxicity mechanism may differ between the total extract group and the other groups through the results of biochemical indicators. The iTRAQ proteomics study showed that hepatotoxicity resulting from PM was mainly related to the abnormal activity of mitochondrion function-related oxidative phosphorylation pathways. Conclusion: This iTRAQ proteomics study revealed that the hepatotoxicity induced by PM is primarily related to the oxidative phosphorylation pathways. NADH dehydrogenase family proteins and Slc16a2 could be potential biomarkers of hepatotoxicity resulting from PM.

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Eighteen cases of liver injury following ingestion of Polygonum multiflorum

Cited by 96 publications

References 17 publications

<i>In Vivo</i> Screening of Traditional Medicinal Plants for Neuroprotective Activity against Aβ42 Cytotoxicity by Using <i>Drosophila</i> Models of Alzheimer’s Disease

<i>In Vivo</i> Screening of Traditional Medicinal Plants for Neuroprotective Activity against Aβ42 Cytotoxicity by Using <i>Drosophila</i> Models of Alzheimer’s Disease

The Anti-hyperlipidemia Effects of Raw <i>Polygonum multiflorum</i> Extract <i>in Vivo</i>

Application of iTRAQ-Based Quantitative Proteomics Approach to Identify Deregulated Proteins Associated with Liver Toxicity Induced by Polygonum Multiflorum in Rats

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