Eicosapentaenoic and docosahexaenoic acid ethyl esters differentially enhance B-cell activity in murine obesity

Teague, Heather; Harris, Mitchel; Fenton, Jenifer I.; Lallemand, Perrine; Shewchuk, Brian M.; Shaikh, Saame Raza

doi:10.1194/jlr.m049809

Cited by 57 publications

(65 citation statements)

References 62 publications

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“…Paradoxically, increased production of the proinflammatory cytokines TNF-α and IL-6 was reported due to increased n-3/n-6 PUFA ratio in the diet [36,37]. In contrast to these reports, a recent meta-analysis of 68 clinical trials with a total of 4601 subjects showed that n-3 PUFA dietary supplementation had a significant lowering effect on blood levels of C-reactive protein, IL-6 and TNF-α [38].…”

Section: Discussionmentioning

confidence: 92%

Endogenous conversion of n-6 to n-3 polyunsaturated fatty acids attenuates K/BxN serum-transfer arthritis in fat-1 mice

Woo

Lim

Park

et al. 2015

The Journal of Nutritional Biochemistry

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Section: Discussionmentioning

confidence: 92%

Endogenous conversion of n-6 to n-3 polyunsaturated fatty acids attenuates K/BxN serum-transfer arthritis in fat-1 mice

Woo

Lim

Park

et al. 2015

The Journal of Nutritional Biochemistry

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“…The nature of the contained PUFA is also of importance as in studies comparing EPA versus DHA containing PE, incorporation into rafts was twofold greater for DHA than for EPA, indicating that the longer acyl chains were favoring intrusion into the dense SM/chol packing of the L o phase [58]. In addition, a recent study in animal with DHA-enriched diet and cultured cells was able to demonstrate that DHA enhances raft formation in plasma cell membranes [59].…”

Section: Tablementioning

confidence: 96%

Docosahexaenoic acid promotes micron scale liquid-ordered domains. A comparison study of docosahexaenoic versus oleic acid containing phosphatidylcholine in raft-like mixtures

Georgieva

Chachaty

Hazarosova

et al. 2015

Biochimica et Biophysica Acta (BBA) - Biomembranes

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The understanding of the functional role of the lipid diversity in biological membranes is a major challenge. Lipid models have been developed to address this issue by using lipid mixtures generating liquid-ordered (Lo)/liquid-disordered (Ld) immiscibility. The present study examined mixtures comprising Egg sphingomyelin (SM), cholesterol (chol) and phosphatidylcholine (PC) either containing docosahexaenoic (PDPC) or oleic acid (POPC). The mixtures were examined in terms of their capability to induce phase separation at the micron- and nano-scales. Fluorescence microscopy, electron spin resonance (ESR), X-ray diffraction (XRD) and calorimetry methods were used to analyze the lateral organization of the mixtures. Fluorescence microscopy of giant vesicles could show that the temperature of the micron-scale Lo/Ld miscibility is higher for PDPC than for POPC ternary mixtures. At 37°C, no micron-scale Lo/Ld phase separation could be identified in the POPC containing mixtures while it was evident for PDPC. In contrast, a phase separation was distinguished for both PC mixtures by ESR and XRD, indicative that PDPC and POPC mixtures differed in micron vs nano domain organization. Compared to POPC, the higher line tension of the Lo domains observed in PDPC mixtures is assumed to result from the higher difference in Lo/Ld order parameter rather than hydrophobic mismatch.

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“…The n-3 PUFA enriched diet contained 1.3% of the total kcal from docosahexaenoic acid (DHA) and 2% from eicosapentaenoic acid (EPA). For a select study, C57BL/6 mice were fed high fat diets supplemented with EPA or DHA ethyl esters, as previously described [13]. Mice were sacrificed via CO 2 inhalation followed by cervical dislocation.…”

Section: Methodsmentioning

confidence: 99%

“…The Bio-Plex was run with Bio-Plex manager 4.1 software. Serum levels of IL-5 were confirmed with an Elisa MAX Standard (Biolegend), as per manufacturer’s protocol [13]. …”

Section: Methodsmentioning

confidence: 99%

“…To exemplify, n-3 PUFAs modify B cell activation, antigen presentation to helper T cells, antibody production, surface expression of select molecules, development in bone marrow, and the relative percentage or frequency of B cells in specific tissues [7-13]. While these studies have established that numerous functional endpoints of B cells are modified with n-3 PUFAs, the underlying mechanisms for the most part remain unknown.…”

Section: Introductionmentioning

confidence: 99%

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Short-term consumption of n-3 PUFAs increases murine IL-5 levels, but IL-5 is not the mechanistic link between n-3 fatty acids and changes in B-cell populations

Teague

Harris

Whelan

et al. 2016

The Journal of Nutritional Biochemistry

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N-3 polyunsaturated fatty acids (PUFA) exert immunomodulatory effects on B cells. We previously demonstrated that n-3 PUFAs enhanced the relative percentage and/or frequency of select B2 cell subsets. The objectives here were to determine if n-3 PUFAs: i) could boost cytokines that target B cell frequency, ii) enhance the frequency of the B1 population and iii) to identify the mechanism by which n-3 PUFAs modify the proportion of B cells. Administration of n-3 PUFAs as fish oil to C57BL/6 mice enhanced secretion of the Th2 cytokine IL-5 but not IL-9 or IL-13. N-3 PUFAs had no influence on the percentage or frequency of peritoneal B1 or B2 cells. Subsequent experiments with IL-5−/− knockout mice showed n-3 PUFAs decreased the percentage of bone marrow B220loIgMhi cells and increased the proportion and number of splenic IgM+IgDloCD21lo cells compared to the control. These results, when compared with our previous findings with wild type mice, suggested IL-5 had no role in mediating the effect of n-3 PUFAs on B cell populations. To confirm this conclusion, we assayed IL-5 secretion in a diet-induced obesity model in which n-3 PUFAs enhanced the frequency of select B cell subsets. N-3 PUFA supplementation as ethyl esters to obesogenic diets did not alter circulating IL-5 levels. Altogether, the data establish that n-3 PUFAs as fish oil can increase circulating IL-5 in lean mice, which has implications for several disease endpoints, but this increase in IL-5 is not the mechanistic link between n-3 PUFAs and changes in B cell populations.

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Eicosapentaenoic and docosahexaenoic acid ethyl esters differentially enhance B-cell activity in murine obesity

Cited by 57 publications

References 62 publications

Endogenous conversion of n-6 to n-3 polyunsaturated fatty acids attenuates K/BxN serum-transfer arthritis in fat-1 mice

Endogenous conversion of n-6 to n-3 polyunsaturated fatty acids attenuates K/BxN serum-transfer arthritis in fat-1 mice

Docosahexaenoic acid promotes micron scale liquid-ordered domains. A comparison study of docosahexaenoic versus oleic acid containing phosphatidylcholine in raft-like mixtures

Short-term consumption of n-3 PUFAs increases murine IL-5 levels, but IL-5 is not the mechanistic link between n-3 fatty acids and changes in B-cell populations

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