Eicosapentaenoic acid modifies lipid composition in caveolae and induces translocation of endothelial nitric oxide synthase

Li, Qiurong; Zhang, Qiang; Wang, Meng; Zhao, Sumin; Ma, Jian; Luo, Nan; Li, Ning; Li, Yousheng; Xu, Guowang; Li, Jieshou

doi:10.1016/j.biochi.2006.10.009

Cited by 90 publications

(60 citation statements)

References 29 publications

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“…Previous reports in the literature suggest that treatment of cells with DHA and EPA causes a partial displacement of caveolin-1 from the lipid rafts and decreases signaling from molecules such as interleukin-1 in human umbilical cord endothelial cells (24,25). Our observations confirmed the previous literature and showed that the phenomenon also occurs in MDA-MB-231 breast cancer cells (Fig.…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Omega-3 Polyunsaturated Fatty Acids Down-Modulate CXCR4 Expression and Function in MDA-MB-231 Breast Cancer Cells

Altenburg

Siddiqui

2009

Molecular Cancer Research

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Section: Discussionsupporting

confidence: 92%

“…Incorporation of DHA and EPA into the plasma membrane leads to disruption of the lipid raft domains (23). One of the markers for lipid rafts, caveolin-1, is partially displaced on treatment with DHA and EPA (24,25). Similarly, n-3 PUFAs have been shown to displace other signaling proteins from lipid rafts (26).…”

Section: Introductionmentioning

confidence: 99%

Omega-3 Polyunsaturated Fatty Acids Down-Modulate CXCR4 Expression and Function in MDA-MB-231 Breast Cancer Cells

Altenburg

Siddiqui

2009

Molecular Cancer Research

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“…It is believed that EPA-derived PGI3 is also involved in NO production. Furthermore, EPA increases NO production without PGI2 as follows: EPA modifies the lipid composition of the caveolae of endothelial cells, promotes the migration of caveolae-bound eNOS to the cytoplasm and finally activates eNOS 59) .…”

Section: Effects Of Pgi2 and Pgi3 On The Vascular Tonementioning

confidence: 99%

Eicosapentaenoic Acid (EPA) Reduces Cardiovascular Events: Relationship with the EPA/Arachidonic Acid Ratio

Ohnishi

Saitō

2013

JAT

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The clinical efficacy of fish oil and high-purity eicosapentaenoic acid ethyl ester (hp-EPA-E) for treating cardiovascular disease (CVD) has been reported. Fish oil contains saturated and monounsaturated fatty acids that have pharmacological effects opposite to those of ω3 fatty acids (ω3). Moreover, ω3, such as EPA and docosahexaenoic acid (DHA), do not necessarily have the same metabolic and biological actions. This has obscured the clinical efficacy of ω3. Recently, the Japan EPA Lipid Intervention Study (JELIS) of hp-EPA-E established the clinical efficacy of EPA for CVD, and higher levels of blood EPA, not DHA, were found to be associated with a lower incidence of major coronary events. A significant reduction in the risk of coronary events was observed when the ratio of EPA to arachidonic acid (AA) (EPA/AA) was ＞0.75. Furthermore, the ratio of prostaglandin (

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“…Li et al reported that cells cultured in eicosapentaenoic acid-rich medium show decreased caveolin-1 and eNOS protein expression in caveolae, along with changes in the FA profiles of the caveolae. 43) They concluded that the microenvironment of the caveola modifies the location and function of proteins in the membrane. Furthermore, in cells cultured in medium containing certain FAs, the properties of ion channels/receptors and signaling pathways are modified by structural changes in the lipid raft/caveolae.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, in cells cultured in medium containing certain FAs, the properties of ion channels/receptors and signaling pathways are modified by structural changes in the lipid raft/caveolae. [43][44][45] This may play a role in hypothermia. We investigated the effects of dietary intervention on changes in BT induced by representative pyrogenic (IL-1β) and hypothermic (Bom) drugs injected into the cerebroventricle to act directly on the CNS.…”

Section: Discussionmentioning

confidence: 99%

Long-term High-Soybean Oil Feeding Alters Regulation of Body Temperature in Rats

Tsushima

Yamada

Miyazawa

et al. 2014

Biological & Pharmaceutical Bulletin

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We investigated whether body temperature (BT) regulatory mechanisms are influenced by dietary fatty acids (FA). Male Wistar rats were fed a high-fat diet containing fish oil (HFD), soybean oil (HSD) or lard (HLD). At the 20-week intervention, the BT of the HSD and HLD groups were lower than that of the normal diet (ND) group in the light and dark periods. The intracerebroventricular injections of interleukin-1β and bombesin in the HSD group induced greater hyperthermia and weaker hypothermia, respectively, than in the ND group. The HSD differentially affected BT under both physiological and pharmacological conditions. In the hypothalamus, the ratio of n-6/n-3 FAs was higher in the HSD group compared with the ND group. DNA microarrays revealed increased expression of thyroid-stimulating hormone β-subunit, and decreased expression of several genes in the hypothalamus of the HSD group compared with the ND group. The HSD feeding increased several adipokine concentrations in the plasma. However, there were no adipokines or gene expressions that changed in only the HSD and HLD groups showing significant hypothermia under the physiological condition. These findings suggested that long-term HSD intake produces abnormal BT regulation. It is less likely that adipokines or proteins/peptides are involved in abnormal BT regulation under the physiological conditions after HSD feeding.Key words body temperature; hypothalamus; high-fat diet; interleukin (IL)-1β; bombesinWe now obtain more energy from fat and glucose than ever before. These changes have induced hyperlipidemia and adipocyte hypertrophy, resulting in the risk of cardiovascular diseases, diabetes mellitus and obesity. From this point of view, there are many reports on lipid and/or glucose metabolisms during high-fat diet feeding in living animals.

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Eicosapentaenoic acid modifies lipid composition in caveolae and induces translocation of endothelial nitric oxide synthase

Cited by 90 publications

References 29 publications

Omega-3 Polyunsaturated Fatty Acids Down-Modulate CXCR4 Expression and Function in MDA-MB-231 Breast Cancer Cells

Omega-3 Polyunsaturated Fatty Acids Down-Modulate CXCR4 Expression and Function in MDA-MB-231 Breast Cancer Cells

Eicosapentaenoic Acid (EPA) Reduces Cardiovascular Events: Relationship with the EPA/Arachidonic Acid Ratio

Long-term High-Soybean Oil Feeding Alters Regulation of Body Temperature in Rats

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