Eicosanoids mediate nodulation reactions to bacterial infections in adults of the cricket, Gryllus assimilis

Miller, Jon D.; Howard, Ralph W.; Rana, Rico L; Tunaz, Hasan; Stanley, David

doi:10.1016/s0022-1910(98)00089-4

Cited by 61 publications

(29 citation statements)

References 23 publications

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“…Tobacco hornworms, M. sexta yielded approximately 120 nodules/individual by 6-h PI (Miller et al, 1994), and silkworms, B. mori, formed approximately 80 nodules/individual at 15-h PI (Stanley-Samuelson et al, 1997). In our work with another hemimetabolous species, Miller et al (1999) recorded approximately 45 nodules at 4-h PI in identical experiments with adult crickets, G. assimilis. Howard et al (1998) suggested that differences in nodulation intensity might be due to differences in circulating hemocyte populations.…”

Section: Discussionsupporting

confidence: 58%

“…1.05 pmol/mg protein/h) as the major product. Rates of prostaglandin biosynthesis recorded from in vitro insect preparations are typically low, and the values recorded with honey bee preparations are similar to values recorded from larvae of the butterfly, Colias eurytheme (Stanley-Samuelson et al, 1997) and adults of cicadas, Magicicada septendecim (Tunaz et al, 1999). Hence, a basic assumption of this line of experimentation has been met.…”

Section: Discussionmentioning

confidence: 58%

“…Using similar experimental protocols, we have obtained comparable results with several insect species, including the tenebrionid beetle, Zophobas atratus (Miller et al, 1996), the silkworm, Bombyx mori (Stanley-Samuelson et al, 1997) the larvae of two other moths, black cutworms, Agrotis ipsilon and true armyworms, Pseudaletia unipuncta (Jurenka et al, 1997), adults of the cricket, Gryllus assimilis (Miller et al, 1999), the cockroach, Periplaneta americana (Tunaz and Stanley, 1999) and the 17-year periodical cicadas, Magicicada septendecim and M. cassini (Tunaz et al, 1999). In related work, Mandato et al (1997) found that cell spreading and prophenoloxidase activation, two distinct phases of nodulation, as well as another cellular defense reaction, phagocytosis, also are mediated by eicosanoids in waxmoths, Galleria mellonella.…”

Section: Introductionmentioning

confidence: 69%

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Eicosanoids act in nodulation reactions to bacterial infections in newly emerged adult honey bees, Apis mellifera, but not in older foragers

Bedick

Tunaz

Aliza

et al. 2001

Comparative Biochemistry and Physiology Part C: Toxicology &

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Nodulation is the first, and qualitatively predominant, cellular defense reaction to bacterial infections in insects. We tested the hypothesis that eicosanoids also mediate nodulation reactions to bacterial challenge in adults of a social insect, the honey bee, Apis mellifera. Treating newly-emerged experimental bees with the eicosanoid biosynthesis inhibitor, dexamethasone, impaired nodulation reactions to bacterial infections, and the influence of dexamethasone was reversed by treating infected insects with arachidonic acid, an eicosanoid precursor. Several other eicosanoid biosynthesis inhibitors, including the cyclooxygenase inhibitor, indomethacin, and the dual cyclooxygenase/lipoxygenase inhibitor, phenidone, also impaired the ability of experimental honeybees to form nodules in reaction to bacterial challenge. The influence of phenidone on nodulation was expressed in a dose-dependent manner. However, in experiments with older honey bees foragers, similar bacterial challenge did not evoke nodulation reactions. We infer from our results that while eicosanoids mediate cellular immune responses to bacterial infections in newly emerged honey bees, and more broadly, in most insect species, nodulation reactions to bacterial challenge probably do not occur in all phases of insect life cycles.

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 69%

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Eicosanoids act in nodulation reactions to bacterial infections in newly emerged adult honey bees, Apis mellifera, but not in older foragers

Bedick

Tunaz

Aliza

et al. 2001

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…Among the roles ascribed to eicosanoids in insects are release of cricket oviposition behavior (Destephano and Brady, 1977;Loher et al, 1981), regulation of fluid secretion rates in Malpighian tubules in mosquitoes and ants (Petzel and Stanley-Samuelson, 1992;Van Kerkhove et al, 1995), regulation of temperature set points in cicadas (Toolson et al, 1994), and modulation of the cellular immune response to bacteria (Stanley-Samuelson et al, 1991). Eicosanoid actions in cellular defense mechanisms have been most intensely investigated (see reviews by Stanley-Samuelson, 1994;Stanley and Howard, 1998;Howard and Stanley, 1999;Stanley, 2000).…”

Section: Introductionmentioning

confidence: 99%

Eicosanoids mediate Manduca sexta cellular response to the fungal pathogen Beauveria bassiana: A role for the lipoxygenase pathway

Lord

Anderson

Stanley

2002

Arch Insect Biochem Physiol

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Many studies have documented the involvement of eicosanoids in insect cellular immune responses to bacteria. The use of the fungal pathogen Beauveria bassiana as a nodulation elicitor, with inhibition of phospholipase A 2 by dexamethasone, extends the principle to fungi. This study also provides the first evidence of involvement of the lipoxygenase (LOX) pathway rather than the cyclooxygenase (COX) pathway in synthesis of the nodulation mediating eicosanoid(s). The LOX product, 5(S)-hydroperoxyeicosa-6E,8Z,11Z,14Z-tetraenoic acid (5-HPETE), substantially reversed nodulation inhibition caused by dexamethasone and the LOX inhibitors, caffeic acid and esculetin. The COX product, prostaglandin H 2 (PGH 2 ), did not reverse the nodulation inhibition by dexamethasone or the COX inhibitor, ibuprofen. None of the inhibitors tested had a significant effect on the phagocytosis of B. bassiana blastospores in vitro. Hemocyte phenoloxidase activity was reduced by dexamethasone, esculetin, and the COX inhibitor, indomethacin. The rescue candidates 5-HPETE and PGH 2 did not reverse the inhibition. Arch.

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“…Eicosanoids are oxygenated metabolites of arachidonic acid and two other C20 polyunsaturated fatty acids. Major groups of eicosanoids include prostaglandins and various lipoxygenase products, and these compounds are thought to mediate cellular and some humoral immune reactions to bacterial infection (Jurenka et al, 1997(Jurenka et al, , 1999Miller et al, 1994Miller et al, , 1996Miller et al, , 1999Morishima et al, 1997;Tunaz et al, 1999;Stanley, 2000). Park and Kim (2000) showed that treating infected larvae of the moth Spodoptera exigua with arachidonic acid reduced the mortality caused by X. nematophilus by 40% and that the arachidonic acid effect was expressed in a dose-dependent manner.…”

Section: Introductionmentioning

confidence: 99%

The bacterium Xenorhabdus nematophilus depresses nodulation reactions to infection by inhibiting eicosanoid biosynthesis in tobacco hornworms, Manduca sexta

Park

Kim

Putnam

et al. 2003

Arch Insect Biochem Physiol

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The bacterium, Xenorhabdus nematophilus, is a virulent insect pathogen. We tested the hypothesis that this bacterium impairs insect cellular immune defense reactions by inhibiting biosynthesis of eicosanoids involved in mediating cellular defense reactions. Fifth instar tobacco hornworms, Manduca sexta, produced melanized nodules in reaction to challenge with living and heat-killed X. nematophilus. However, the nodulation reactions were much attenuated in insects challenged with living bacteria (approximately 20 nodules/larva for living bacteria vs. approximately 80 nodules/larva in insects challenged with heat-killed bacteria). The nodule-inhibiting action of living X. nematophilus was due to a factor that was present in the organic, but not aqueous, fraction of the bacterial cultural medium. The nodule-inhibiting factor in the organic fraction was labile to heat treatments. The immunodepressive influence of the factor in the organic fraction was reversed by treating challenged hornworms with arachidonic acid. The factor also depressed nodulation reactions to challenge with the plant pathogenic bacteria, Pseudomonas putida and Ralstonia solanacearum. These findings indicate that one or more factors from X. nematophilus depress nodulation reactions in tobacco hornworms by inhibiting eicosanoid biosynthesis.

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Eicosanoids mediate nodulation reactions to bacterial infections in adults of the cricket, Gryllus assimilis

Cited by 61 publications

References 23 publications

Eicosanoids act in nodulation reactions to bacterial infections in newly emerged adult honey bees, Apis mellifera, but not in older foragers

Eicosanoids act in nodulation reactions to bacterial infections in newly emerged adult honey bees, Apis mellifera, but not in older foragers

Eicosanoids mediate Manduca sexta cellular response to the fungal pathogen Beauveria bassiana: A role for the lipoxygenase pathway

The bacterium Xenorhabdus nematophilus depresses nodulation reactions to infection by inhibiting eicosanoid biosynthesis in tobacco hornworms, Manduca sexta

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