Ehrlichia-like 16S rDNA Sequence from Wild White-Tailed Deer (Odocoileus virginianus)

Dawson, Jacqueline E.; Warner, Cynthia K.; Baker, Vanesa; Ewing, S. A.; Stallknecht, David E.; Davidson, William R.; Kocan, A. Alan; Lockhart, J. Mitchell; Olson, James G.

doi:10.2307/3284115

Cited by 65 publications

(61 citation statements)

References 8 publications

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“…To our knowledge, three of these variants have not earlier been identified in sheep, and two of them have not been identified in any other study before. Nucleotide differences at 16S rRNA level in A. phagocytophila have been found in isolates from rodents, deer, and Ixodes ticks (3,5,6,15,17,20,23,32,33). However, whether all variants can cause disease in humans and animals remains to be determined.…”

Section: Discussionmentioning

confidence: 99%

Identification ofAnaplasma phagocytophila(FormerlyEhrlichia phagocytophila) Variants in Blood from Sheep in Norway

Stuen

Pol²,

Bergström

et al. 2002

J Clin Microbiol

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A total of 41 blood samples were collected from 40 Anaplasma phagocytophila-infected sheep in 11 sheep flocks from four different counties of southern Norway. The presence and nature of the Anaplasma species were identified by microscopic detection of morulae, PCR, reverse line blot hybridization, and 16S rRNA gene sequencing. A. phagocytophila was identified in all of the samples, and sequencing of the 16S rRNA gene revealed the presence of four variants of A. phagocytophila. Two of these variants have been described before, but two were newly identified 16S rRNA variants of this species. A. phagocytophila variant 1 was found in nine flocks, A. phagocytophila variant 2 was found in four flocks, the A. phagocytophila prototype was found in two flocks, and A. phagocytophila variant 5 was found in one flock. In two flocks, some sheep were infected with A. phagocytophila variant 1, whereas others were infected with A. phagocytophila variant 2, and in three animals a double infection with two variants was registered. Analyses of the blood samples revealed that blood from sheep infected with A. phagocytophila variant 2 contained nearly twice as many neutrophils and eight times as many Anaplasma-infected neutrophils as blood from sheep infected with the A. phagocytophila variant 1. Furthermore, only 43% of the A. phagocytophila variant 2-infected sheep displayed antibody responses in an immune fluorescence assay, whereas 93% of the sheep with the A. phagocytophila variant 1-infected sheep were seropositive.

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Section: Discussionmentioning

confidence: 99%

Identification ofAnaplasma phagocytophila(FormerlyEhrlichia phagocytophila) Variants in Blood from Sheep in Norway

Stuen

Pol²,

Bergström

et al. 2002

J Clin Microbiol

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show abstract

“…of WTD) (Dawson et al, 1996;Belongia et al, 1997;Lockhart et al, 1997a;Yabsley et al, 2002Yabsley et al, , 2003Arens et al, 2003;Moore et al, 2003). Four of these species are known to be zoonotic, and WTD have been shown to be competent reservoirs of E. chaffeensis and PM Ehrlichia sp.…”

Section: Discussionmentioning

confidence: 99%

Natural and Experimental Infection of White-Tailed Deer (Odocoileus Virginianus) From the United States With an Ehrlichia Sp. Closely Related to Ehrlichia Ruminantium

Yabsley

Loftis

Little

2008

Journal of Wildlife Diseases

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ABSTRACT:An Ehrlichia sp. (Panola Mountain [PM] Ehrlichia sp.) closely related to Ehrlichia ruminantium was recently detected in a domestic goat experimentally infested with lone star ticks (LSTs, Amblyomma americanum) collected from Georgia, USA. The infected goat exhibited pyrexia and mild clinical pathologic abnormalities consistent with ehrlichiosis. At least two other Ehrlichia species (Ehrlichia chaffeensis and Ehrlichia ewingii) are maintained in nature by a cycle involving LSTs as the primary vector and white-tailed deer (Odocoileus virginanus) as a known or suspected reservoir. To investigate the possibility that white-tailed deer are potential hosts of the PM Ehrlichia sp., whole blood samples collected from 87 wild deer from 2000 to 2002 were screened with a species-specific nested PCR assay targeting the citrate synthase gene. In addition, two laboratory-raised white-tailed deer fawns were each infested with 120 wild-caught LST adults from Missouri, USA, and blood samples were periodically collected and tested for the PM Ehrlichia sp. Of 87 deer tested from 20 locations in the southeastern United States, three (3%) deer from Arkansas, North Carolina, and Virginia were positive for the PM Ehrlichia sp. Wildcaught ticks transmitted the PM Ehrlichia sp. to one of two deer fawns, and colony-reared nymphal LSTs acquired the organism from the deer, maintained it transstadially as they molted to adults, and transmitted the PM Ehrlichia sp. to two naïve fawns. These findings indicate that white-tailed deer are naturally and experimentally susceptible to infection with an Ehrlichia sp. closely related to E. ruminantium and are able to serve as a source of infection to LSTs.

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“…The region of the 16S gene selected for Ehrlichia identification and the reaction conditions used for PCR amplification of that region have been described previously. 23 In this study, however, Roche PCR buffer and Taq polymerase were used. The primary amplification reaction used primers 15F 23 and HE3 9 in a 100-L reaction containing 20 L of template DNA.…”

Section: Patientmentioning

confidence: 99%

Tissue diagnosis of Ehrlichia chaffeensis in patients with fatal ehrlichiosis by use of immunohistochemistry, in situ hybridization, and polymerase chain reaction.

Dawson¹,

Paddock²,

Warner³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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Abstract. In the United States, human ehrlichiosis is a complex of emerging tick-borne diseases caused by 3 distinct Ehrlichia species: Ehrlichia chaffeensis, Ehrlichia ewingii, and the human granulocytotropic ehrlichiosis agent. Ehrlichioses are characterized by a mild to severe illness, and ϳ 4% of cases are fatal. Because these obligate intracellular bacteria are difficult to resolve with routine histologic techniques, their distribution in tissues has not been well described. To facilitate the visualization and detection of ehrlichiae, immunohistochemistry (IHC), in situ hybridization (ISH), and polymerase chain reaction (PCR) assays were developed by use of tissues from 4 fatal cases of E. chaffeensis infection. Evidence of E. chaffeensis via IHC, ISH, and PCR was documented in all 4 cases. Abundant immunostaining and in situ nucleic acid hybridization were observed in spleen and lymph node from all 4 patients. Significantly, in 2 of these patients, serologic evidence of infection was absent. Use of IHC, ISH, and PCR to visualize and detect Ehrlichia in tissues can facilitate diagnosis of ehrlichial infections.

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Ehrlichia-like 16S rDNA Sequence from Wild White-Tailed Deer (Odocoileus virginianus)

Cited by 65 publications

References 8 publications

Identification ofAnaplasma phagocytophila(FormerlyEhrlichia phagocytophila) Variants in Blood from Sheep in Norway

Identification ofAnaplasma phagocytophila(FormerlyEhrlichia phagocytophila) Variants in Blood from Sheep in Norway

Natural and Experimental Infection of White-Tailed Deer (Odocoileus Virginianus) From the United States With an Ehrlichia Sp. Closely Related to Ehrlichia Ruminantium

Tissue diagnosis of Ehrlichia chaffeensis in patients with fatal ehrlichiosis by use of immunohistochemistry, in situ hybridization, and polymerase chain reaction.

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