Ehrlichia canis TRP36 diversity in naturally infected-dogs from an urban area of Colombia

Arroyave, Esteban; Rodas-González, Juan D.; Zhang, Xiaofeng; Labruna, Marcelo Bahia; González, María Soledad; Silva, Jorge Arturo Fernández; McBride, Jere W.

doi:10.1016/j.ttbdis.2019.101367

Cited by 19 publications

(30 citation statements)

References 38 publications

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“…Due to the absence of haemorrhage on MR images, the multiple lesions found in our case could be related to plasma cell infiltration of the central nervous system or vasculopathy 9,11 . Other common clinical signs such as fever, lymphadenomegaly, splenomegaly and weight loss were not present in our case 8,14 . CSF analysis demonstrated similarities to previously published cases 1,9,11 .…”

Section: Discussionsupporting

confidence: 55%

“…CME is a multisystemic disease, resulting in a great variety of clinical signs 5 . The infection may manifest in acute, subclinical or chronic forms; 6,7 however, these three clinical phases can be difficult to distinguish due to the similarity of their clinical signs 8 …”

Section: Introductionmentioning

confidence: 99%

“…5 The infection may manifest in acute, subclinical or chronic forms; 6,7 however, these three clinical phases can be difficult to distinguish due to the similarity of their clinical signs. 8 Common clinical signs include fever, anorexia, depression, lethargy, generalized lymphadenomegaly, splenomegaly and ocular abnormalities (such as anterior and posterior uveitis). 1,3,4,6,7 Common haematological abnormalities are hyperproteinaemia, hyperglobulinaemia, hypoalbuminaemia, mild elevation of alkaline phosphatase (ALP) and alanine aminotransferase (ALT) activities.…”

Section: Introductionmentioning

confidence: 99%

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Magnetic resonance findings of meningoencephalitis in a dog seroreactive to Ehrlichia canis in United Kingdom

Dona¹,

Gomes²,

Andriuc

et al. 2021

Vet Record Case Reports

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We describe a novel clinical presentation and imaging findings in a dog seropositive to Ehrlichia canis. A dog presented with an acute non‐ambulatory tetraparesis and severe obtundation. MRI findings showed multifocal intra‐axial changes centered in the grey matter. The lesions caused mild mass effect and leptomeningeal contrast enhancement. The dog responded to treatment with the progressive reduction of antibody titers detected on the indirect fluorescent antibody test and complete resolution of the clinical signs. Canine monocytic ehrlichiosis should be considered a differential in dogs with thrombocytopenia and meningoencephalitis.

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Section: Discussionsupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Magnetic resonance findings of meningoencephalitis in a dog seroreactive to Ehrlichia canis in United Kingdom

Dona¹,

Gomes²,

Andriuc

et al. 2021

Vet Record Case Reports

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“…Although in our study, the taxon of R. sanguineus was not identified, we expect that it belongs to the tropical lineage considering the geographic location and that E. canis was the most prevalent tick-borne pathogen in both dogs (IFA: 82% PCR: 18%) and ticks (PCR: 30.4%). In addition, our previous results showed the presence of three genotypes of E. canis (strain United States, Brazil, and Costa Rica) infecting dogs in Colombia, in which one of these strains has been associated with human infection in Costa Rica (Arroyave et al, 2020;Bouza-Mora et al, 2017).…”

Section: Discussionmentioning

confidence: 88%

Detection of tick-borne rickettsial pathogens in naturally infected dogs and dog-associated ticks in Medellin, Colombia

Arroyave

Cornwell

McBride

et al. 2020

Rev. Bras. Parasitol. Vet.

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Tick-borne rickettsial pathogens (TBRP) are important causes of infections in both dogs and humans. Dogs play an important role as a biological host for several tick species and can serve as sentinels for rickettsial infections. Our aim was to determine the presence of TBRP in dogs and in dog-associated ticks and their potential risk to human diseases in Medellin, Colombia. DNA for E. canis (16S rRNA and dsb) and A. platys (groEl) was detected in 17.6% (53/300) and 2.6% (8/300) of dogs, respectively. Antibodies against Ehrlichia spp. 82 (27.3%) and Anaplasma spp. 8 (2.6%) were detected in dogs. Antibody reactivity against both agents were found in 16 dogs (5.3%). Eight dogs showed antibody for Rickettsia spp. with titers that suggest 3 of them had a probable exposure to R. parkeri. Rhipicephalus sanguineus s.l. (178/193) was the main tick in dogs, followed by R. microplus (15/193). The minimum infection rates (MIR) in R. sanguineus were 11.8% for E. canis and 3.4% for A. platys. E. canis and A. platys are the main TBRP infecting dogs and ticks and R. sanguineus s.l. is likely involved in the transmission of both agents. Interestingly, we found serological evidence of exposure in dogs for spotted fever group rickettsiae.

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“…Three different genotypes based on amino acid sequences of the TR of gp36 included E. canis US TRP36 (TEDSVSAPATEDSVSAPA) ( Doyle et al., 2006 ), Brazil (BR) TRP36 (ASVVPEAEASVVPEAEASVVPEAE) ( Aguiar et al., 2013 ) and Costa Rica (CR) TRP36 (EASVVPAAEAPQPAQQTEDEFFSDGIEA). These variations of the TR sequences serologically distinguished different E. canis genotypes and revealed coinfection or multiple sequential infections of these genotypes ( Aguiar et al., 2016 ; Arroyave et al., 2020 ). In our Thailand study, the Maximum-likelihood phylogenetic analysis of gp36 revealed three different genotypes, including subgroup A, B, and C ( Figure 1 ).…”

Section: Discussionmentioning

confidence: 96%

Detection of specific IgM and IgG antibodies in acute canine monocytic ehrlichiosis that recognize recombinant gp36 antigens

Kaewmongkol

Suwan

Sirinarumitr

et al. 2020

Heliyon

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The efficacy of antibody detection tools for all stages of Ehrlichia canis infections and for various genotypes remains unclear. We produced recombinant gp36 (rgp36) antigens from different isolates of Thai E. canis to confirm the immunoreactivities to these recombinant proteins from naturally infected dogs. Sera and blood samples were taken from 21 dogs naturally infected with E. canis and in the clinical stages of acute phase ehrlichiosis. The expression vectors and competent E. coli produced two isolates of rgp36. These two major rgp36s were recognized by the dogs' sera in Western blotting, with both anti-dog IgM and IgG used as secondary antibodies. The two different genotypes of these local recombinant immunoreactive proteins were gp36 subgroup A (isolate 1055) and subgroup B (isolate 533). The Western blot analyses successfully identified both specific IgM and IgG from the dogs' sera. Of all 21 cases, five dogs presented specific IgM, twenty dogs presented specific IgG, and the commercial test used found fifteen seropositive dogs. There were four dogs that presented both specific IgM and IgG. Only one dog presented specific IgM only. This report is the first identification of a specific IgM in dogs in response to acute infections with E. canis . The recombinant gp36 isolates may be useful as potential antigenic material for subsequent serological tests that have a high possibility for differentiating between acute, chronic, primary, and nonprimary infections with E. canis .

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Ehrlichia canis TRP36 diversity in naturally infected-dogs from an urban area of Colombia

Cited by 19 publications

References 38 publications

Magnetic resonance findings of meningoencephalitis in a dog seroreactive to Ehrlichia canis in United Kingdom

Magnetic resonance findings of meningoencephalitis in a dog seroreactive to Ehrlichia canis in United Kingdom

Detection of tick-borne rickettsial pathogens in naturally infected dogs and dog-associated ticks in Medellin, Colombia

Detection of specific IgM and IgG antibodies in acute canine monocytic ehrlichiosis that recognize recombinant gp36 antigens

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