Ehrlichia canis in dogs of Mexico: Prevalence, incidence, co–infection and factors associated

Ojeda-Chí, Melina Maribel; Rodríguez-Vivas, Róger Iván; Esteve-Gasent, Maria D.; León, Adalberto A. Pérez de; Modarelli, Joseph J.; Villegas-Perez, Sandra Luz

doi:10.1016/j.cimid.2019.101351

Cited by 24 publications

(22 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…From the 80 seropositive pet dogs included in this study, in 36, successful amplifi cation of the specifi c fragment within the 16S rRNA of E. canis, was obtained. The established prevalence of 45.0%, was similar to the prevalence observed in molecular studies conducted in several countries from different continents [16][17][18][19][20][21]. However, some studies report a rather low prevalence of E. canis (Malaysia -2%, Myanmar -0.75%, etc.…”

Section: Resultssupporting

confidence: 82%

“…However, the use of PCR for the detection of E. canis in the subclinical and chronic form may have limited value due to the low concentration of the microorganism in the circulation, which can result in false-negative results [14,15]. Molecular studies conducted around the world presented E.canis prevalence of 41.59% in India, 28.0% in Pakistan, 29.26% in Mexico, 37.0% in the Caribbean island of Saint Kitts, 34.50% in Northeastern Brazil, and 22.0% in Portugal [16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia

et al. 2021

View full text Add to dashboard Cite

Canine monocytic ehrlichiosis (CME) is a widespread, tick-borne, canine disease, caused by an obligate intracellular bacterium, Ehrlichia canis. The main vector, a brown-dog tick, Rhipicephalus sanguineus, is widely distributed, especially in areas with tropic, subtropic, or Mediterranean climates (Central and South America, Eastern and Western Asia, Africa, Australia and Southern Europe). The study performed in 2012, by Stefanovska et al., determined a seroprevalence of 18.7% of E. canis among the Macedonian dog population. Up to date, the presence of E. canis, using molecular diagnostic methods, has not been investigated in Macedonia. Therefore, this study aimed to confirm the presence of E. canis, in the pet-dog population on the territory of the city of Skopje, North Macedonia, using a highly sensitive multiplex Real-Time PCR method (qPCR). Whole blood samples from 80 dogs of different breeds and ages, with clinical symptoms of CME and positive serology result for the presence of antibodies against E.canis, were collected for analyses. Out of 80 dogs, 36 (45%) were found as positive. The present work reports the first molecular detection of E. canis in pet dogs on the territory of the city of Skopje, Macedonia.

show abstract

Section: Resultssupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Canine babesiosis did not take enough consideration in research work in Egypt compared with other animal species [ 27 ]. Other studies investigated that the prevalence of E. canis in dogs was 10.3% in Korea [ 28 ], 1.3% in Southern China [ 29 ], 44.6% in North Trinidad [ 30 ], 29.26% in Mexico [ 31 ], and 40.6% in Colombia [ 32 ]. Although our study contradicts with reports on age difference regarding positivity to E. canis , there was a significant increase in E. canis infection rate in older dogs (7 years of age and older), which suggests that acquiring infection increases with age [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Prevalence, genetic, and biochemical evaluation of immune response of police dogs infected with Babesia vogeli

et al. 2021

View full text Add to dashboard Cite

Background and Aim: Babesia species are tick-borne protozoan parasites of apicomplexan type which infect the erythrocytes of dogs it ranges from subclinical to severe cases, depending on different factors such as immune status, age, and presence of other co-infections with the Babesia species. Hence, this study aimed to identify the protozoan parasites infecting police dogs of different breeds, ages, and both sexes in Egypt. Concerning molecular detection of Babesia vogeli using conventional polymerase chain reaction sequencing and phylogenetic analysis, followed by the assessment of immunological and biochemical status of infected dogs. Materials and Methods: The blood of 242 police K9 dogs was collected. The age, breed, sex, and health status with clinical signs of dogs were recorded. Hematological, biochemical, and oxidative stress analyses of the blood were performed together with gene expression analysis using two genes (gamma interferon [IFN-γ] and tumor necrosis factor-alpha [TNF- α]). The identification of the causative agent was performed using molecular analysis of the 18S ribosomal RNA (rRNA). The 18S rRNA region of canine Babesia spp. was successfully amplified, and sequencing data were deposited in GenBank (accession number: MT565474.1), which resembled those of B. vogeli. Results: The results of blood samples screening revealed that of the 242 blood samples, 62 were positive for B. vogeli infection. The infection rate in male dogs was higher than that in female dogs. The police dogs were classified into the following three groups of dogs: (1st group) healthy, (2nd infected with B. vogeli, and mixed infection of B. vogeli and Ehrlichia canis). The oxidative stress biomarkers levels in B. vogeli infected dogs were greater than that of healthy dogs. Likewise, IFN-γ and TNF-α level in B. vogeli infected dogs were elevated in infected dogs. Conclusion: Our findings demonstrated that B. vogeli had completely adverse effects on the health condition of the police dogs that may lead to death in some dogs.

show abstract

“…En el 2019, se realizó un estudio de detección molecular de E. canis en zonas rurales de Yucatán, encontrando un 29.26% de prevalencia (Ojeda-Chi et al, 2019), lo cual es cercano a lo reportado en este trabajo (26.8%); pero mucho mayor comparada con la prevalencia encontrada en perros evaluados en la Comarca Lagunera (4%) (Almazán et al, 2016).…”

Section: Discussionunclassified

Detección molecular de Ehrlichia canis y Anaplasma phagocytophilum y alteraciones hematológicas de perros infectados

et al. 2021

View full text Add to dashboard Cite

show abstract

Ehrlichia canis in dogs of Mexico: Prevalence, incidence, co–infection and factors associated

Cited by 24 publications

References 34 publications

Molecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia

Molecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia

Prevalence, genetic, and biochemical evaluation of immune response of police dogs infected with Babesia vogeli

Detección molecular de Ehrlichia canis y Anaplasma phagocytophilum y alteraciones hematológicas de perros infectados

Contact Info

Product

Resources

About